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meganucleases

Candidate: toolkit item1 source documents5 linked claims
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Aliases

MNs

Evidence Snippets

programmable site-specific nucleases, including ... meganucleases (MNs)
Evidence 1Source 1DOIPubMedprovenance

Supporting Sources

Linked Claims

Claim 1comparative property summarysupports2017Source 1DOIPubMed

The biology of each genome-editing nuclease influences targeting potential, off-target cleavage spectrum, ease of use, and the types of recombination events produced at targeted double-strand breaks.

Quoted textsource-backed
However, the underlying biology of each genome-editing nuclease influences the targeting potential, the spectrum of off-target cleavages, the ease-of-use, and the types of recombination events at targeted double-strand breaks.
Claim 2mechanistic summarysupports2017Source 1DOIPubMed

Targeting double-strand breaks to user-defined genomic locations greatly enhances DNA repair event rates relative to uncatalyzed events at the same sites.

Quoted textsource-backed
By targeting double-strand breaks to user-defined locations, the rates of DNA repair events are greatly enhanced relative to un-catalyzed events at the same sites.
Claim 3review scope statementsupports2017Source 1DOIPubMed

The review focuses on diversity of nuclease domains for genome editing and on biochemical properties and applications best suited to each domain.

Quoted textsource-backed
Here, we focus on the diversity of nuclease domains available for genome editing, highlighting biochemical properties and the potential applications that are best suited to each domain.
Claim 4review summarysupports2017Source 1DOIPubMed

Programmable site-specific nucleases including ZFNs, TALENs, meganucleases, and CRISPR-associated proteins have enabled and accelerated genome editing.

Quoted textsource-backed
Breakthroughs in the development of programmable site-specific nucleases, including zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), meganucleases (MNs), and most recently, the clustered regularly interspaced short palindromic repeats (CRISPR) associated proteins (including Cas9) have greatly enabled and accelerated genome editing.
Claim 5scope limitationsupports2017Source 1DOIPubMed

No single genome-editing nuclease is optimized for all possible applications.

Quoted textsource-backed
No single genome-editing nuclease is optimized for all possible applications.