we constructed two light-controlled E. coli gene expression systems, OptoE.coliLight system and OptoE.coliDark system
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OptoE.coliDark system
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The OptoE.coliDark system was applied to protein production and metabolic flux control.
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We applied the OptoE.coliDark system to protein production and metabolic flux control.
OptoE.coliLight and OptoE.coliDark enable bifunctional gene expression regulation in E. coli through light manipulation and show superior controllability compared to IPTG-induced systems.
Quoted textsource-backed
These systems enable bifunctional gene expression regulation in E. coli through light manipulation and show superior controllability compared to IPTG-induced systems.
Dark-induced production of 1,3-propanediol exceeded 110% of IPTG-induced production and dark-induced production of ergothioneine exceeded 60% of IPTG-induced production.
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the titers of dark-induced production of 1,3-propanediol (1,3-PDO) and ergothioneine exceeded 110% and 60% of those induced by IPTG, respectively
Protein production levels with the OptoE.coliDark system are comparable to those induced by IPTG.
Quoted textsource-backed
Protein production levels are comparable to those induced by IPTG.
Building on OptoLacI, the authors constructed two light-controlled E. coli gene expression systems named OptoE.coliLight and OptoE.coliDark.
Quoted textsource-backed
Building upon OptoLacI, we constructed two light-controlled E. coli gene expression systems, OptoE.coliLight system and OptoE.coliDark system.