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First-pass extracted concept

PchlFJSC1

Candidate: toolkit item1 source documents5 linked claims
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Aliases

PchlFJSC1, PchlFJSC1 promoter

Extracted Explainers

What the tool is doing

PchlFJSC1 is a promoter that drives reporter expression in response to far-red light in Chlorogloeopsis fritschii PCC 9212. The abstract states that it is specifically activated by far-red light and can drive eyfp expression.

Source 1DOIPubMed

Resources required

Its use requires a gene-expression setup in C. fritschii PCC 9212 and exposure to far-red light, with EYFP used as the reported readout in the abstract. The study also mentions a conjugation-based technique for expressing the reporter gene in this host.

Source 1DOIPubMed

What problem it solves

It offers a light-controlled alternative to chemical induction for cyanobacterial gene expression, which the abstract frames as advantageous for industrial-scale use where chemical addition is costly or environmentally unfriendly.

Source 1DOIPubMed

What it does not solve

The abstract does not show portability across many cyanobacterial strains or performance with cargos other than eyfp. It also does not provide detailed construct architecture or quantitative burden data.

Source 1DOIPubMed

Alternatives

The abstract contrasts this system with cyanobacterial inducible promoters controlled by chemical compounds and notes that only a green-light-inducible cyanobacterial expression system had previously been described.

Source 1DOIPubMed

Evidence Snippets

This promoter, PchlFJSC1, was successfully used to drive eyfp expression. PchlFJSC1 is tightly regulated by light quality (i.e., wavelength) and leads to an approximately 30-fold increase in EYFP production when cells were exposed to far-red light.
Evidence 1Source 1DOIPubMedprovenance

Supporting Sources

Linked Claims

Claim 1dynamic controlsupports2023Source 1DOIPubMed

Induction from PchlFJSC1 depends on far-red light intensity and stops after return to visible light.

Quoted textsource-backed
The induction level was controlled by the far-red light intensity, and induction stopped when cells were returned to visible light.
Claim 2induction specificitysupports2023Source 1DOIPubMed

PchlFJSC1 is specifically activated by far-red light and tightly regulated by light quality.

Quoted textsource-backed
We also identified a promoter specifically activated by far-red light... PchlFJSC1 is tightly regulated by light quality (i.e., wavelength)
Claim 3performancesupports2023Source 1DOIPubMed

Far-red light exposure produces approximately 30-fold higher EYFP production from PchlFJSC1.

Quoted textsource-backed
PchlFJSC1 ... leads to an approximately 30-fold increase in EYFP production when cells were exposed to far-red light.
Claim 4system summarysupports2023Source 1DOIPubMed

The study developed a functional far-red-light-regulated gene-expression system for Chlorogloeopsis fritschii PCC 9212.

Quoted textsource-backed
Collectively, this study developed a functional gene-expression system for C. fritschii PCC 9212 that can be regulated by exposing cells to far-red light.
Claim 5tool functionsupports2023Source 1DOIPubMed

PchlFJSC1 can drive eyfp expression in Chlorogloeopsis fritschii PCC 9212.

Quoted textsource-backed
This promoter, PchlFJSC1, was successfully used to drive eyfp expression.