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PHOT1a phototropin 1 from Avena sativa

Candidate: toolkit item1 source documents5 linked claims
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Aliases

PHOT1a, phototropin 1

Evidence Snippets

S27, S30, S274, S300, S317, S325, S332, and S349 of the PHOT1a sequence of Avena sativa
Evidence 1Source 1DOIPubMedprovenance

Supporting Sources

Linked Claims

Claim 1domain localizationsupports2003Source 1DOIPubMed

In Avena sativa PHOT1a, the N-terminus upstream of LOV1 and the hinge region between LOV1 and LOV2 are autophosphorylation regions.

Quoted textsource-backed
We describe here two additional domains, the N-terminus upstream of LOV1 and the hinge region between LOV1 and LOV2, as the regions for autophosphorylation
Claim 2enzyme specificity comparisonsupports2003Source 1DOIPubMed

Protein kinase A phosphorylation of recombinant phototropin domains had the same site specificity as the phototropin kinase.

Quoted textsource-backed
phosphorylation of recombinant domains by protein kinase A, which turned out to have the same site specificity as the phototropin kinase
Claim 3fluence dependencesupports2003Source 1DOIPubMed

In vivo, serines close to the LOV1 domain are phosphorylated at lower blue-light fluence than serines close to the LOV2 domain.

Quoted textsource-backed
Investigation of the autophosphorylation in vivo revealed that serines close to the LOV1 domain are phosphorylated at lower fluence of blue light than the serines close to the LOV2 domain.
Claim 4mechanistic interpretationsupports2003Source 1DOIPubMed

Recovery of phosphorylation in vivo during a dark period after saturating irradiation is caused by dephosphorylation rather than by degradation of phosphorylated phototropin and new synthesis of nonphosphorylated phototropin.

Quoted textsource-backed
Recovery of phosphorylation in vivo during a dark period after saturating irradiation is caused by dephosphorylation rather than by degradation of the phosphorylated form and new synthesis of nonphosphorylated phototropin.
Claim 5site mappingsupports2003Source 1DOIPubMed

The autophosphorylation sites in Avena sativa PHOT1a were identified as S27, S30, S274, S300, S317, S325, S332, and S349.

Quoted textsource-backed
the phosphorylation sites were identified by site-directed mutagenesis as S27, S30, S274, S300, S317, S325, S332, and S349 of the PHOT1a sequence of Avena sativa