We developed a photo-inactivatable bioluminescent indicator, based on a combination of luciferase-fragment complementation and a photoreaction of a light, oxygen, and voltage domain from Avena sativa Phototropin1 (LOV2)
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photo-inactivatable bioluminescent indicator
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Bioluminescence imaging using the indicator enabled long-time recording of acidification during apoptotic and autophagous processes in a cell population and during ischemic conditions in living mice.
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Bioluminescence imaging, taken as an index of the recovery rates, enabled long-time recording of acidification in apoptotic and autophagous processes in a cell population and an ischemic condition in living mice.
The indicator technology is broadly applicable for sensing organelle-specific acidic changes in target biological tissues.
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This technology using the indicator is widely applicable to sense organelle-specific acidic changes in target biological tissues.
Bioluminescence of the indicator decreases upon light irradiation and gradually recovers in the dark state.
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Bioluminescence of the indicator diminished upon light irradiation and it recovered gradually in the dark state thereafter.
The indicator recovery rate is highly sensitive to pH changes and is not susceptible to fluctuations in luciferin or ATP concentrations.
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The recovery rate was remarkably sensitive to pH changes but unsusceptible to fluctuation of luciferin or ATP concentrations.
The authors developed a photo-inactivatable bioluminescent indicator to visualize temporally dynamic intracellular acidification in living tissue samples.
Quoted textsource-backed
We developed a photo-inactivatable bioluminescent indicator, based on a combination of luciferase-fragment complementation and a photoreaction of a light, oxygen, and voltage domain from Avena sativa Phototropin1 (LOV2), to visualize temporally dynamic acidification in living tissue samples.