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First-pass extracted concept

plasmid interference with CRISPR interference (PICI) system

Candidate: toolkit itemType: assay method1 source documents2 linked claims
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Aliases

PICI system

Extracted Explainers

What the tool is doing

The PICI system is a plasmid interference/CRISPR interference platform used to rapidly characterize anti-CRISPR proteins and support development of Acr-based tools. The abstract presents it as a versatile screening system.

Source 1DOIPubMed

Resources required

The abstract supports that the system is established in Escherichia coli and involves plasmid interference with CRISPR interference. Further construct or assay details are not given in the abstract.

Source 1DOIPubMed

What problem it solves

It addresses the otherwise long and tedious process of characterizing novel anti-CRISPR proteins and exploiting Acr-related technologies.

Source 1DOIPubMed

Alternatives

The abstract does not name a direct alternative screening system, but contrasts the PICI system with slower conventional characterization workflows.

Source 1DOIPubMed

Evidence Snippets

Here, we established a versatile plasmid interference with CRISPR interference (PICI) system in Escherichia coli for rapidly characterizing Acrs and developing Acr-based technologies.
Evidence 1Source 1DOIPubMedprovenance

Supporting Sources

Linked Claims

Claim 1discoverysupports2023Source 1DOIPubMed

AcrIIA33 and AcrIIA34 are novel type II-A anti-CRISPR proteins discovered using the PICI system.

Quoted textsource-backed
Utilizing the PICI system, we discovered two novel type II-A Acrs (AcrIIA33 and AcrIIA34)
Claim 2method capabilitysupports2023Source 1DOIPubMed

The PICI system is a versatile screening system in Escherichia coli for rapidly characterizing anti-CRISPR proteins and developing Acr-based technologies.

Quoted textsource-backed
Here, we established a versatile plasmid interference with CRISPR interference (PICI) system in Escherichia coli for rapidly characterizing Acrs and developing Acr-based technologies.