RecV recombinase system for spatiotemporally controlled light-inducible genomic modifications
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RecV recombinase system
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Aliases
light-inducible recombinases
Evidence Snippets
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Linked Claims
Light-inducible recombinases can produce highly targeted, sparse, and strong labeling of individual neurons enabling whole-brain morphological reconstruction to identify axonal projection specificity.
Quoted textsource-backed
we showed that light-inducible recombinases can produce highly targeted, sparse and strong labeling of individual neurons thereby enabling whole-brain morphological reconstruction to identify their axonal projection specificity
The method was applied in deep brain targets with a demonstration of functional calcium imaging.
Quoted textsource-backed
In addition to targeting cortical brain areas, we applied the method in deep targets, with a demonstration of functional calcium imaging.
The light-inducible recombinases can induce genomic modifications in live mouse brains under one-photon or two-photon light induction.
Quoted textsource-backed
We generated viral vectors to express these light-inducible recombinases and demonstrated that they can induce genomic modifications in dense or sparse populations of neurons in live mouse brains controlled by one-photon or two-photon light induction.
These molecular tools enable spatiotemporally precise targeted genomic modifications for neural circuit analysis linking genetic identity, morphology, connectivity, and function.
Quoted textsource-backed
These molecular tools enable spatiotemporally-precise, targeted genomic modifications that will greatly facilitate detailed analysis of neural circuits and linking genetic identity, morphology, connectivity and function.