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Streptococcus pyogenes Cas9

Candidate: toolkit itemType: multi component switch1 source documents8 linked claims
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Aliases

Cas9, CRISPR-Cas9, SpCas9, S py Cas9

Evidence Snippets

Here, recent protein-engineering approaches for expanding the versatility of the Streptococcus pyogenes Cas9 (SpCas9) is reviewed
Evidence 1Source 1DOIPubMedprovenance

Supporting Sources

Linked Claims

Claim 1application scopesupports2018Source 1DOIPubMed

CRISPR-Cas9 has been used across applications including gene therapy, gene regulation, epigenome modification, and chromosome imaging.

Quoted textsource-backed
CRISPR-Cas9 has been used in a wide variety of applications ranging from basic science to the clinic, such as gene therapy, gene regulation, modifying epigenomes, and imaging chromosomes.
Claim 2impactsupports2018Source 1DOIPubMed

These Cas9 limitations hinder the use of CRISPR for disease treatment and wider biotechnological applications.

Quoted textsource-backed
These obstacles hinder the use of CRISPR for disease treatment and in wider biotechnological applications.
Claim 3limitationsupports2018Source 1DOIPubMed

Cas9 can exhibit aberrant off-target activity.

Quoted textsource-backed
(ii) aberrant off-target activity
Claim 4limitationsupports2018Source 1DOIPubMed

Cas9 has a strict dependence on a protospacer-adjacent motif sequence.

Quoted textsource-backed
some limitations have also been reported, for instance (i) a strict dependence on a protospacer-adjacent motif (PAM) sequence
Claim 5limitationsupports2018Source 1DOIPubMed

Cas9 lacks sufficient modulation of protein binding and endonuclease activity for precise spatiotemporal control.

Quoted textsource-backed
(iv) lack of modulation of protein binding and endonuclease activity, which is crucial for precise spatiotemporal control of gene expression or genome editing
Claim 6limitationsupports2018Source 1DOIPubMed

The large size of Cas9 creates problems for CRISPR delivery.

Quoted textsource-backed
(iii) the large size of Cas9 is problematic for CRISPR delivery
Claim 7review focussupports2018Source 1DOIPubMed

The review emphasizes domain fusion or splitting, rational design, and directed evolution as protein-engineering strategies for expanding SpCas9 versatility.

Quoted textsource-backed
Here, recent protein-engineering approaches for expanding the versatility of the Streptococcus pyogenes Cas9 (SpCas9) is reviewed, with an emphasis on studies that improve or develop novel protein functions through domain fusion or splitting, rational design, and directed evolution.
Claim 8strategy overviewsupports2018Source 1DOIPubMed

Protein-engineering approaches are presented as solutions to overcome Cas9 limitations and generate more robust and efficient DNA manipulation tools.

Quoted textsource-backed
Protein-engineering approaches offer solutions to overcome the limitations of Cas9 and generate robust and efficient tools for customized DNA manipulation.