whole-cell patch clamp

Whole-cell patch clamp was used to record outward currents from CFTR-expressing HEK293T cells during ultrasound stimulation. In this paper it serves as the direct functional assay for ultrasound-responsive chloride-channel activity.

Whole-cell patch clamp was used to determine voltage-gated K+ current in Jcam lymphoma cells after Klotho treatment.

The abstract supports the need for exogenous CFTR expression in HEK293T cells, whole-cell patch-clamp recording, and ultrasound delivery. Pharmacologic perturbation with GlyH101 and Forskolin and chloride-containing solution conditions were also part of assay interpretation.

The reported use required Jcam lymphoma cells and electrophysiology after 24 hour incubation with or without recombinant human Klotho protein.

It allows the authors to test whether low-intensity ultrasound evokes membrane currents consistent with CFTR activity at the single-cell level.

It tests whether the Klotho-dependent current effect also appears in a lymphoma cell context rather than only in oocytes.

The abstract does not show that patch clamp alone resolves the detailed gating mechanism or broader in vivo neuromodulation performance.

The abstract does not state that this assay by itself proves the molecular mechanism of current increase.

No direct alternative assay is named in the abstract.

The paper also used dual electrode voltage clamp in Xenopus oocytes and flow cytometry for Kv1.3 abundance.