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Wor1 valency-increased engineered strains

Candidate: toolkit itemType: construct pattern1 source documents4 linked claims
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Aliases

engineered strains with increased Wor1 valency

Extracted Explainers

What the tool is doing

This engineered construct pattern increases the interaction valency of the Candida albicans transcription factor Wor1. The abstract states that doing so increases white-opaque switching frequency and condensate propensity.

Source 1DOIPubMed

Resources required

Implementation requires engineered strains in which Wor1 valency is increased, either by adding extra prion-like domains or by forced dimerization. The abstract also indicates evaluation in vitro and in mammalian cells for condensate propensity.

Source 1DOIPubMed

What problem it solves

It provides an experimental way to test whether increasing transcription-factor interaction valency can alter cell fate determination. In this paper it is used to connect Wor1 valency to switching and phase separation.

Source 1DOIPubMed

What it does not solve

The abstract does not show that this approach solves broader control of all white-opaque regulators or all fungal cell-fate systems. It also does not provide enough detail to recover exact construct architectures.

Source 1DOIPubMed

Alternatives

The paper contrasts valency engineering with gene-dosage perturbation using simple and complex haploinsufficiency analyses. Within valency engineering, the abstract names extra PrLD addition and forced dimerization as alternative implementations.

Source 1DOIPubMed

Evidence Snippets

Engineered strains with increased Wor1 valency, either via the addition of extra prion-like domains (PrLDs) or by forced dimerization, increased switching frequencies by up to two orders of magnitude.
Evidence 1Source 1DOIPubMedprovenance

Supporting Sources

Linked Claims

Claim 1engineering outcomesupports2025Source 1DOIPubMed

Increasing Wor1 valency by adding extra prion-like domains or by forced dimerization increased white-opaque switching frequency by up to two orders of magnitude.

Claim 2mechanistic linksupports2025Source 1DOIPubMed

Changes to transcription factor gene dosage and valency can alter cell fate determination, and these changes are linked to propensity for condensate formation.

Claim 3phase separation associationsupports2025Source 1DOIPubMed

Increasing Wor1 valency increased its propensity to form phase-separated condensates in vitro and in mammalian cells.

Claim 4valency effectsupports2025Source 1DOIPubMed

Wor1 function is highly sensitive to interaction valency.