heavy-ion mutagenesis of elf3-1 seeds
Selection basis: Generate mutant lines from elf3-1 seeds using heavy-ion beams.
Enriches for: mutational diversity
First-pass extracted concept
heavy-ion mutagenesis suppressor screen in elf3-1 under continuous light
Candidate: workflow template1 source documents10 linked claims4 stage observations
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Workflow Stage Observations
screen for delayed flowering under continuous light but not under long or short days
Selection basis: Identify mutation(s) that delayed flowering under LL but not LDs or SDs even without ELF3.
Higher fidelity: yes
Enriches for: late flowering under continuous light, photoperiod-specific phenotype
Guards against: mutations causing delayed flowering under long days, mutations causing delayed flowering under short days
Preserves downstream axes: specificity to continuous light response in the elf3 background
candidate line identification
Advance criteria: Lines identified in the screen were retained as suppressor candidates.
genetic analysis of candidate sel20
Selection basis: Determine the genetic lesion underlying sel20.
Higher fidelity: yes
Evidence Snippets
elf3-1 seeds were mutagenized with heavy-ion beams and used to identify mutation(s) that delayed flowering under LL but not long days (LDs) or SDs even without ELF3. In this screening, seven candidate lines named suppressor of elf3 1 (self1), sel3, sel5, sel7, sel14, sel15, and sel20 were identified.
Supporting Sources
Linked Claims
The late-flowering phenotype in elf3;sel20 and lhy;cca1 is affected by the presence of darkness.
Quoted textsource-backed
These results indicated that the late-flowering phenotype in the double mutant elf3;sel20 as well as in lhy;cca1 was affected by the presence of darkness.
CRY2 may play a more essential role in accelerating flowering under continuous light than under long days or short days.
Quoted textsource-backed
The results suggest that CRY2 may play more essential roles in the acceleration of flowering under LL than LDs or SDs.
The late-flowering phenotype of lhy;cca1 mutants under continuous light is partially suppressed by svp, flc, or elf3.
Quoted textsource-backed
the late-flowering phenotype of lhy;cca1 mutants was partially suppressed by svp, flc, or elf3
lhy;cca1 double mutants accelerate flowering under short days but delay flowering under continuous light.
Quoted textsource-backed
lhy;cca1 double mutants with severe defects in circadian rhythms showed accelerated flowering under short days (SDs), but delayed flowering under continuous light (LL).
The elf3;sel20 double mutant shows a late-flowering phenotype and decreased FT expression under continuous light but not under short days or long days.
Quoted textsource-backed
A late-flowering phenotype and decrease of FT expression in the elf3;sel20 double mutant was obvious under LL but not under SDs or LDs.
The inverted flowering response of lhy;cca1 under continuous light may involve both ELF3-SVP/FLC-dependent and independent pathways.
Quoted textsource-backed
These results suggest that the unique mechanism of the inversion of the flowering response of lhy;cca1 under LL may involve both the ELF3-SVP/FLC-dependent and -independent pathways.
SVP protein level increases in lhy;cca1 mutants under continuous light.
Quoted textsource-backed
The protein level of the floral repressor SVP increased in lhy;cca1 mutants under LL
elf3 suppresses SVP accumulation in lhy;cca1 under continuous light.
Quoted textsource-backed
elf3 suppressed the SVP accumulation in lhy;cca1 under LL
sel20 is a new deletion allele of a mutation in CRY2.
Quoted textsource-backed
Genetic analysis indicated that sel20 was a new deletion allele of a mutation in the blue light receptor, CRY2.
ELF3 interacts with both CCA1 and SVP.
Quoted textsource-backed
ELF3 interacted with both CCA1 and SVP