First-pass extracted concept
Workflow Stage Observations
Selection basis: isolate mutants that overexpress CAB genes in the dark using a transgenic line with two CAB3 promoter-reporter fusions
Enriches for: dark derepression of CAB expression
Guards against: reporter-specific aberration affecting only one CAB3 promoter
Preserves downstream axes: normal dark-grown morphology
Selection basis: aberrant expression of both CAB3 promoters
Higher fidelity: yes
Enriches for: concordant reporter deregulation across both CAB3 promoter fusions
Guards against: single-reporter artifacts
Evidence Snippets
Supporting Sources
Linked Claims
Epistasis studies suggest that DOC1 and DET3 act downstream from DET1 on two separate branches in the phototransduction pathway, whereas DOC2 appears to act on a distinct pathway from DET1.
Epistasis studies suggest that DOC1 and DET3 act downstream from DET1 on two separate branches in the phototransduction pathway. In contrast, DOC2 appears to act on a distinct pathway from DET1.
doc mutations are more specific for derepressing CAB expression than det1 and det2, which affect CAB and RBCS expression to approximately the same extent.
Unlike det1 and det2 mutants, which affect the expression of CAB and RBCS to approximately the same extent, all three doc mutations are much more specific in derepressing the expression of CAB.
Most doc mutations are recessive and define at least three loci: doc1, doc2, and doc3.
Genetic and phenotypic analyses indicate that most of the mutations are recessive and define at least three loci (doc1, doc2, doc3).
Mutations in doc1, doc2, or doc3 impair plant growth under short-day conditions.
Mutations in doc1, doc2, or doc3 also impair plant growth under short-day conditions.
Morphological changes can be genetically separated from changes in CAB gene expression, and CAB gene regulation can be separated further from RBCS gene regulation.
The phenotypes of doc mutants suggest that morphological changes can be genetically separated from changes in CAB gene expression. Moreover, the regulation of CAB gene expression can be separated further from the regulation of RBCS gene expression.
The isolated doc mutants have normal etiolated morphology in the dark.
All of the mutants have normal etiolated morphology in the dark.
A screen using a transgenic CAB3 promoter-reporter line isolated eight mutants with aberrant expression of both CAB3 promoters in the dark, designated doc mutants.
A screen was designed to isolate mutants that overexpressed the CAB genes in the dark, by use of transgenic line containing a T-DNA construct with two CAB3 promoter-reporter fusions. Eight mutants that showed aberrant expression of both CAB3 promoters were isolated and were designated doc mutants