Source 1primary paper2020Nature Communications
Toolkit/AAV-PA-Cre 3.0
AAV-PA-Cre 3.0
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
AAV-PA-Cre 3.0 is an adeno-associated viral delivery resource for the photoactivatable Cre recombinase 3.0 system, generated and validated for in vivo mouse applications. It delivers a blue-light-gated Cre/lox recombination system engineered for mammalian expression with reduced background recombination.
Usefulness & Problems
Why this is useful
This resource enables viral delivery of a light-controlled Cre/lox recombination system for mouse experiments, supporting spatiotemporal control of recombination in vivo. The reported PA-Cre 3.0 modifications reduce dark leak activity while improving blue-light induction, which is useful when basal recombination must be minimized.
Problem solved
It addresses the problem of background recombination in photoactivatable Cre systems, which was linked to high sequence similarity in the dimerization domains. It also addresses the need for a mammalian-expression-optimized, AAV-deliverable PA-Cre configuration for in vivo mouse use.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A delivery strategy grouped with the mechanism branch because it determines how a system is instantiated and deployed in context.
Techniques
No technique tags yet.
Target processes
recombinationImplementation Constraints
PA-Cre 3.0 was optimized for mammalian expression using a CAG promoter, Magnets, and a 2A self-cleaving peptide. Codon modifications were introduced for mouse gene targeting and viral production to reduce background recombination associated with sequence similarity in the dimerization domains; the evidence only states that an AAV resource was generated and validated, without further delivery details.
The supplied evidence does not specify AAV serotype, packaging configuration, illumination parameters, target tissues, or quantitative recombination performance. Independent replication is not provided in the supplied evidence, and validation details beyond the statement that the resource was generated and validated are not described.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Codon modifications for mouse gene targeting and viral production were introduced to prevent background recombination caused by high sequence similarity in the dimerization domains.
To prevent background recombination caused by the high sequence similarity in the dimerization domains, we modify the codons for mouse gene targeting and viral production.
Codon modifications for mouse gene targeting and viral production were introduced to prevent background recombination caused by high sequence similarity in the dimerization domains.
To prevent background recombination caused by the high sequence similarity in the dimerization domains, we modify the codons for mouse gene targeting and viral production.
Codon modifications for mouse gene targeting and viral production were introduced to prevent background recombination caused by high sequence similarity in the dimerization domains.
To prevent background recombination caused by the high sequence similarity in the dimerization domains, we modify the codons for mouse gene targeting and viral production.
Codon modifications for mouse gene targeting and viral production were introduced to prevent background recombination caused by high sequence similarity in the dimerization domains.
To prevent background recombination caused by the high sequence similarity in the dimerization domains, we modify the codons for mouse gene targeting and viral production.
Codon modifications for mouse gene targeting and viral production were introduced to prevent background recombination caused by high sequence similarity in the dimerization domains.
To prevent background recombination caused by the high sequence similarity in the dimerization domains, we modify the codons for mouse gene targeting and viral production.
Codon modifications for mouse gene targeting and viral production were introduced to prevent background recombination caused by high sequence similarity in the dimerization domains.
To prevent background recombination caused by the high sequence similarity in the dimerization domains, we modify the codons for mouse gene targeting and viral production.
Codon modifications for mouse gene targeting and viral production were introduced to prevent background recombination caused by high sequence similarity in the dimerization domains.
To prevent background recombination caused by the high sequence similarity in the dimerization domains, we modify the codons for mouse gene targeting and viral production.
PA-Cre 3.0 was optimized for mammalian expression using a CAG promoter, Magnets, and a 2A self-cleaving peptide.
To improve PA-Cre technology, we compare light-dimerization tools and optimize for mammalian expression using a CAG promoter, Magnets, and 2A self-cleaving peptide.
PA-Cre 3.0 was optimized for mammalian expression using a CAG promoter, Magnets, and a 2A self-cleaving peptide.
To improve PA-Cre technology, we compare light-dimerization tools and optimize for mammalian expression using a CAG promoter, Magnets, and 2A self-cleaving peptide.
PA-Cre 3.0 was optimized for mammalian expression using a CAG promoter, Magnets, and a 2A self-cleaving peptide.
To improve PA-Cre technology, we compare light-dimerization tools and optimize for mammalian expression using a CAG promoter, Magnets, and 2A self-cleaving peptide.
PA-Cre 3.0 was optimized for mammalian expression using a CAG promoter, Magnets, and a 2A self-cleaving peptide.
To improve PA-Cre technology, we compare light-dimerization tools and optimize for mammalian expression using a CAG promoter, Magnets, and 2A self-cleaving peptide.
PA-Cre 3.0 was optimized for mammalian expression using a CAG promoter, Magnets, and a 2A self-cleaving peptide.
To improve PA-Cre technology, we compare light-dimerization tools and optimize for mammalian expression using a CAG promoter, Magnets, and 2A self-cleaving peptide.
PA-Cre 3.0 was optimized for mammalian expression using a CAG promoter, Magnets, and a 2A self-cleaving peptide.
To improve PA-Cre technology, we compare light-dimerization tools and optimize for mammalian expression using a CAG promoter, Magnets, and 2A self-cleaving peptide.
PA-Cre 3.0 was optimized for mammalian expression using a CAG promoter, Magnets, and a 2A self-cleaving peptide.
To improve PA-Cre technology, we compare light-dimerization tools and optimize for mammalian expression using a CAG promoter, Magnets, and 2A self-cleaving peptide.
The reported modifications significantly reduce dark leak activity and improve blue-light induction in PA-Cre 3.0.
Overall, these modifications significantly reduce dark leak activity and improve blue-light induction developing our new version, PA-Cre 3.0.
The reported modifications significantly reduce dark leak activity and improve blue-light induction in PA-Cre 3.0.
Overall, these modifications significantly reduce dark leak activity and improve blue-light induction developing our new version, PA-Cre 3.0.
The reported modifications significantly reduce dark leak activity and improve blue-light induction in PA-Cre 3.0.
Overall, these modifications significantly reduce dark leak activity and improve blue-light induction developing our new version, PA-Cre 3.0.
The reported modifications significantly reduce dark leak activity and improve blue-light induction in PA-Cre 3.0.
Overall, these modifications significantly reduce dark leak activity and improve blue-light induction developing our new version, PA-Cre 3.0.
The reported modifications significantly reduce dark leak activity and improve blue-light induction in PA-Cre 3.0.
Overall, these modifications significantly reduce dark leak activity and improve blue-light induction developing our new version, PA-Cre 3.0.
The reported modifications significantly reduce dark leak activity and improve blue-light induction in PA-Cre 3.0.
Overall, these modifications significantly reduce dark leak activity and improve blue-light induction developing our new version, PA-Cre 3.0.
The reported modifications significantly reduce dark leak activity and improve blue-light induction in PA-Cre 3.0.
Overall, these modifications significantly reduce dark leak activity and improve blue-light induction developing our new version, PA-Cre 3.0.
The authors generated and validated AAV-PA-Cre 3.0 and two mouse lines that can conditionally express PA-Cre 3.0.
As a resource, we have generated and validated AAV-PA-Cre 3.0 as well as two mouse lines that can conditionally express PA-Cre 3.0.
The authors generated and validated AAV-PA-Cre 3.0 and two mouse lines that can conditionally express PA-Cre 3.0.
As a resource, we have generated and validated AAV-PA-Cre 3.0 as well as two mouse lines that can conditionally express PA-Cre 3.0.
The authors generated and validated AAV-PA-Cre 3.0 and two mouse lines that can conditionally express PA-Cre 3.0.
As a resource, we have generated and validated AAV-PA-Cre 3.0 as well as two mouse lines that can conditionally express PA-Cre 3.0.
The authors generated and validated AAV-PA-Cre 3.0 and two mouse lines that can conditionally express PA-Cre 3.0.
As a resource, we have generated and validated AAV-PA-Cre 3.0 as well as two mouse lines that can conditionally express PA-Cre 3.0.
The authors generated and validated AAV-PA-Cre 3.0 and two mouse lines that can conditionally express PA-Cre 3.0.
As a resource, we have generated and validated AAV-PA-Cre 3.0 as well as two mouse lines that can conditionally express PA-Cre 3.0.
The authors generated and validated AAV-PA-Cre 3.0 and two mouse lines that can conditionally express PA-Cre 3.0.
As a resource, we have generated and validated AAV-PA-Cre 3.0 as well as two mouse lines that can conditionally express PA-Cre 3.0.
The authors generated and validated AAV-PA-Cre 3.0 and two mouse lines that can conditionally express PA-Cre 3.0.
As a resource, we have generated and validated AAV-PA-Cre 3.0 as well as two mouse lines that can conditionally express PA-Cre 3.0.
This paper reports a new version of photoactivatable Cre recombinase called PA-Cre 3.0.
Here, we report the new version of genetically encoded photoactivatable (PA) Cre recombinase, PA-Cre 3.0.
This paper reports a new version of photoactivatable Cre recombinase called PA-Cre 3.0.
Here, we report the new version of genetically encoded photoactivatable (PA) Cre recombinase, PA-Cre 3.0.
This paper reports a new version of photoactivatable Cre recombinase called PA-Cre 3.0.
Here, we report the new version of genetically encoded photoactivatable (PA) Cre recombinase, PA-Cre 3.0.
This paper reports a new version of photoactivatable Cre recombinase called PA-Cre 3.0.
Here, we report the new version of genetically encoded photoactivatable (PA) Cre recombinase, PA-Cre 3.0.
This paper reports a new version of photoactivatable Cre recombinase called PA-Cre 3.0.
Here, we report the new version of genetically encoded photoactivatable (PA) Cre recombinase, PA-Cre 3.0.
This paper reports a new version of photoactivatable Cre recombinase called PA-Cre 3.0.
Here, we report the new version of genetically encoded photoactivatable (PA) Cre recombinase, PA-Cre 3.0.
This paper reports a new version of photoactivatable Cre recombinase called PA-Cre 3.0.
Here, we report the new version of genetically encoded photoactivatable (PA) Cre recombinase, PA-Cre 3.0.
Approval Evidence
1 source1 linked approval claimfirst-pass slug aav-pa-cre-3-0
As a resource, we have generated and validated AAV-PA-Cre 3.0
Source:
resource generationsupports
The authors generated and validated AAV-PA-Cre 3.0 and two mouse lines that can conditionally express PA-Cre 3.0.
As a resource, we have generated and validated AAV-PA-Cre 3.0 as well as two mouse lines that can conditionally express PA-Cre 3.0.
Source:
Comparisons
Source-backed strengths
The source states that AAV-PA-Cre 3.0 was generated and validated as a resource for in vivo mouse applications. Reported engineering changes, including codon modifications and an optimized mammalian expression design, significantly reduced dark leak activity and improved blue-light induction in PA-Cre 3.0.
Ranked Citations
- 1.