Source 1primary paper2025MED
Toolkit/AAV-PHP.(S+eB)
AAV-PHP.(S+eB)
Also known as: AAV-PHP.(S + eB)
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
we generated AAV with mosaic capsid, AAV-PHP.(S + eB), by co-packaging the AAV with two engineered capsid variants: AAV-PHP.eB and AAV-PHP.S
Usefulness & Problems
No literature-backed usefulness or problem-fit explainer has been materialized for this record yet.
Published Workflows
Objective: Design an AAV vector that can efficiently transduce both central and peripheral nervous system compartments while reducing off-target liver transduction.
Why it works: The workflow combines a CNS-tropic parent capsid and a PNS-tropic parent capsid into a mosaic vector, with the expectation that the resulting particles can capture both targeting behaviors while reducing off-target transduction.
mosaic capsid assembly by co-packaging two engineered capsid variantsco-packaging engineered AAV capsid variantssystemic in vivo administrationcomparative benchmarking against parental vectors
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A delivery strategy grouped with the mechanism branch because it determines how a system is instantiated and deployed in context.
Techniques
Computational DesignTarget processes
recombinationValidation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successMouseapplication demomouse
Inferred from claim claim2 during normalization. Systemic administration of AAV-PHP.(S+eB) in adult mice mediates widespread CNS transgene expression comparable to AAV-PHP.eB while also achieving robust dorsal root ganglia neuron transduction similar to AAV-PHP.S. Derived from claim claim2. Quoted text: Systemic administration of AAV-PHP.(S + eB) in adult mice mediated widespread transgene expression throughout the CNS, comparable to AAV-PHP.eB, while simultaneously achieving robust transduction of dorsal root ganglia neurons, similar to AAV-PHP.S.
Source:
successMouseapplication demomouseliver
Inferred from claim claim3 during normalization. AAV-PHP.(S+eB) shows significantly reduced off-target liver transduction compared with both parental vectors. Derived from claim claim3. Quoted text: the mosaic vector demonstrated significantly reduced off-target transduction in the liver compared to both parental vectors, suggesting an improved safety
Source:
Supporting Sources
Ranked Claims
Systemic administration of AAV-PHP.(S+eB) in adult mice mediates widespread CNS transgene expression comparable to AAV-PHP.eB while also achieving robust dorsal root ganglia neuron transduction similar to AAV-PHP.S.
Systemic administration of AAV-PHP.(S + eB) in adult mice mediated widespread transgene expression throughout the CNS, comparable to AAV-PHP.eB, while simultaneously achieving robust transduction of dorsal root ganglia neurons, similar to AAV-PHP.S.
Mosaic capsid assembly is presented as a strategy for designing dual-tropic AAV vectors without increasing viral dose.
These results indicate that mosaic capsid assembly is a potent strategy for designing dual-tropic AAV vectors without increasing viral dose.
AAV-PHP.(S+eB) is a mosaic AAV generated by co-packaging the engineered capsid variants AAV-PHP.eB and AAV-PHP.S.
we generated AAV with mosaic capsid, AAV-PHP.(S + eB), by co-packaging the AAV with two engineered capsid variants: AAV-PHP.eB and AAV-PHP.S
AAV-PHP.(S+eB) shows significantly reduced off-target liver transduction compared with both parental vectors.
the mosaic vector demonstrated significantly reduced off-target transduction in the liver compared to both parental vectors, suggesting an improved safety
Approval Evidence
1 source4 linked approval claimsfirst-pass slug aav-php-s-eb
we generated AAV with mosaic capsid, AAV-PHP.(S + eB), by co-packaging the AAV with two engineered capsid variants: AAV-PHP.eB and AAV-PHP.S
Source:
comparative performancesupports
Systemic administration of AAV-PHP.(S+eB) in adult mice mediates widespread CNS transgene expression comparable to AAV-PHP.eB while also achieving robust dorsal root ganglia neuron transduction similar to AAV-PHP.S.
Systemic administration of AAV-PHP.(S + eB) in adult mice mediated widespread transgene expression throughout the CNS, comparable to AAV-PHP.eB, while simultaneously achieving robust transduction of dorsal root ganglia neurons, similar to AAV-PHP.S.
Source:
design principlesupports
Mosaic capsid assembly is presented as a strategy for designing dual-tropic AAV vectors without increasing viral dose.
These results indicate that mosaic capsid assembly is a potent strategy for designing dual-tropic AAV vectors without increasing viral dose.
Source:
engineering strategysupports
AAV-PHP.(S+eB) is a mosaic AAV generated by co-packaging the engineered capsid variants AAV-PHP.eB and AAV-PHP.S.
we generated AAV with mosaic capsid, AAV-PHP.(S + eB), by co-packaging the AAV with two engineered capsid variants: AAV-PHP.eB and AAV-PHP.S
Source:
safety related observationsupports
AAV-PHP.(S+eB) shows significantly reduced off-target liver transduction compared with both parental vectors.
the mosaic vector demonstrated significantly reduced off-target transduction in the liver compared to both parental vectors, suggesting an improved safety
Source:
Comparisons
No literature-backed comparison notes have been materialized for this record yet.
Ranked Citations
- 1.