Source 1primary paper2026PMC
Toolkit/acyclovir-responsive RNA switch
acyclovir-responsive RNA switch
Also known as: acyclovir-responsive allosteric switch
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The abstract indicates an acyclovir-responsive allosteric switch.
Usefulness & Problems
Why this is useful
This RNA switch is described as an acyclovir-responsive allosteric control module associated with the scaffold platform. It enables switchable behavior in the condensate engineering system.; small-molecule control of RNA scaffold behavior; switchable condensate engineering
Source:
This RNA switch is described as an acyclovir-responsive allosteric control module associated with the scaffold platform. It enables switchable behavior in the condensate engineering system.
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small-molecule control of RNA scaffold behavior
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switchable condensate engineering
Problem solved
It provides a way to externally control RNA scaffold function rather than relying only on constitutive assembly.; adds external small-molecule responsiveness to RNA scaffold systems
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It provides a way to externally control RNA scaffold function rather than relying only on constitutive assembly.
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adds external small-molecule responsiveness to RNA scaffold systems
Problem links
adds external small-molecule responsiveness to RNA scaffold systems
LiteratureIt provides a way to externally control RNA scaffold function rather than relying only on constitutive assembly.
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It provides a way to externally control RNA scaffold function rather than relying only on constitutive assembly.
Published Workflows
Programmable and Switchable RNA Scaffolds for Synthetic Condensate Engineering in Mammalian Cells
2026Objective: Engineer programmable and switchable RNA scaffolds for synthetic condensate formation in mammalian cells.
Why it works: The source scaffold indicates that nanostar-derived RNA scaffolds can be expressed in mammalian cells and that condensate formation depends on dsRNA-stem-driven assembly, while an acyclovir-responsive switch adds external control.
double-stranded RNA stem-driven assemblyallosteric small-molecule-responsive switchingTornado-based RNA expression/circularization
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: actuatorswitch architecture: recruitmentswitch architecture: uncaging
Use requires the engineered RNA switch module and acyclovir as the inducing small molecule.; requires the corresponding RNA switch module; requires acyclovir as the control input
The RNA scaffold platform shows limited orthogonality in cells.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
An acyclovir-responsive allosteric RNA switch enables switchable control within the scaffold platform.
Nanostar-derived RNA scaffolds are used as a platform for synthetic condensate engineering in mammalian cells.
The RNA scaffold platform supports client recruitment.
The RNA scaffold platform shows limited orthogonality in cells.
Condensate assembly in mammalian cells is driven by double-stranded RNA stems.
The scaffold platform uses Tornado-based expression and circularization for deployment in cells.
Approval Evidence
1 source1 linked approval claimfirst-pass slug acyclovir-responsive-rna-switch
The abstract indicates an acyclovir-responsive allosteric switch.
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control featuresupports
An acyclovir-responsive allosteric RNA switch enables switchable control within the scaffold platform.
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Comparisons
Source-stated alternatives
The source scaffold contrasts switchable control with non-switchable scaffold designs in related nanostar condensate papers.
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The source scaffold contrasts switchable control with non-switchable scaffold designs in related nanostar condensate papers.
Source-backed strengths
responsive to acyclovir; supports switchable control
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responsive to acyclovir
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supports switchable control
Compared with joining proteins in creative ways
acyclovir-responsive RNA switch and joining proteins in creative ways address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: conformational_uncaging
Compared with o-nitrobenzyl-caged fluorescein conjugate
acyclovir-responsive RNA switch and o-nitrobenzyl-caged fluorescein conjugate address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: conformational_uncaging
Strengths here: looks easier to implement in practice.
Compared with UNC10245092
acyclovir-responsive RNA switch and UNC10245092 address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: conformational_uncaging
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Ranked Citations
- 1.