Source 1primary paper2018ChemBioChem
Toolkit/adeno-associated virus (AAV) particles
adeno-associated virus (AAV) particles
Also known as: AAV particles
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Adeno-associated virus (AAV) particles were used as a delivery harness for the BphP1-QPAS1-based TA optogenetic system in neurons. In the cited ChemBioChem study, this application was enabled by the small size of the QPAS1 component.
Usefulness & Problems
Why this is useful
AAV particles were useful in this context because they enabled neuronal delivery of the TA system built from BphP1 and QPAS1. The evidence specifically links this utility to the compact size of QPAS1, which made AAV-based construct design feasible.
Problem solved
This delivery harness addressed the packaging and delivery problem for introducing the BphP1-QPAS1 TA optogenetic system into neurons. The cited study indicates that the small QPAS1 component solved a size-related constraint that otherwise would have complicated AAV design.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A delivery strategy grouped with the mechanism branch because it determines how a system is instantiated and deployed in context.
Mechanisms
viral deliveryTechniques
Computational DesignTarget processes
No target processes tagged yet.
Implementation Constraints
The available evidence states only that AAV particles were designed to deliver the TA system to neurons because QPAS1 is small. No additional implementation details are provided for serotype, promoter, genome configuration, production method, or dosing.
The supplied evidence does not report quantitative AAV performance metrics such as titer, transduction efficiency, payload architecture, or expression levels. Applicability of the broader BphP1-QPAS1 toolset depended on cell-type-specific physiological properties such as nuclear transport, which could constrain performance in some contexts.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.
We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.
We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.
We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.
We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.
We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.
We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.
We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
The NIR-light-sensing component of iRIS performed well in all tested cell types.
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
The NIR-light-sensing component of iRIS performed well in all tested cell types.
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
The NIR-light-sensing component of iRIS performed well in all tested cell types.
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
The NIR-light-sensing component of iRIS performed well in all tested cell types.
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
The NIR-light-sensing component of iRIS performed well in all tested cell types.
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
The NIR-light-sensing component of iRIS performed well in all tested cell types.
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
The NIR-light-sensing component of iRIS performed well in all tested cell types.
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Approval Evidence
1 source1 linked approval claimfirst-pass slug adeno-associated-virus-aav-particles
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
Source:
delivery enabling propertysupports
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
Source:
Comparisons
Source-backed strengths
The reported strength is practical compatibility with neuronal delivery of the TA system via AAV particles. The study also tested BphP1-QPAS1-based optogenetic tools in several mammalian cell types including cortical neurons, supporting at least some use in neuronal and non-neuronal settings.
Ranked Citations
- 1.