Source 1primary paper2020Nature Communications
Toolkit/adeno-associated virus delivery
adeno-associated virus delivery
Also known as: AAV delivery
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Adeno-associated virus delivery is a viral gene delivery harness used to deploy the far-red light-induced split-Cre recombinase (FISC) system in vivo. In the cited study, AAV delivery enabled implementation of optogenetically controlled genome engineering in living systems.
Usefulness & Problems
Why this is useful
This delivery harness is useful because it provides an in vivo route for introducing the FISC optogenetic recombination system. In the cited mouse study, this supported spatiotemporally controlled, non-invasive genome engineering when combined with far-red light activation.
Problem solved
AAV delivery addresses the practical problem of getting the FISC genetic components into living tissues for in vivo operation. The supplied evidence supports successful deployment of FISC by AAV, but does not further specify packaging strategy, tropism, or dosing details.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A delivery strategy grouped with the mechanism branch because it determines how a system is instantiated and deployed in context.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: externally suppliedimplementation constraint: context specific validationimplementation constraint: payload burdenoperating role: deliveryswitch architecture: split
The available evidence indicates use of adeno-associated virus to deliver the FISC system in vivo, in a mouse context from the cited study. No additional practical details are provided in the supplied text regarding serotype selection, promoter design, genome configuration, production method, or administration route.
The evidence only states that AAV delivery was used successfully for FISC deployment and does not report vector serotype, cargo architecture, expression levels, or quantitative delivery performance. Independent replication and generalization beyond this single reported application are not established by the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The FISC system expands the optogenetic toolbox for DNA recombination to enable spatiotemporally controlled, non-invasive genome engineering in living systems.
Thus, the FISC system expands the optogenetic toolbox for DNA recombination to achieve spatiotemporally controlled, non-invasive genome engineering in living systems.
The FISC system expands the optogenetic toolbox for DNA recombination to enable spatiotemporally controlled, non-invasive genome engineering in living systems.
Thus, the FISC system expands the optogenetic toolbox for DNA recombination to achieve spatiotemporally controlled, non-invasive genome engineering in living systems.
The FISC system expands the optogenetic toolbox for DNA recombination to enable spatiotemporally controlled, non-invasive genome engineering in living systems.
Thus, the FISC system expands the optogenetic toolbox for DNA recombination to achieve spatiotemporally controlled, non-invasive genome engineering in living systems.
The FISC system expands the optogenetic toolbox for DNA recombination to enable spatiotemporally controlled, non-invasive genome engineering in living systems.
Thus, the FISC system expands the optogenetic toolbox for DNA recombination to achieve spatiotemporally controlled, non-invasive genome engineering in living systems.
The FISC system expands the optogenetic toolbox for DNA recombination to enable spatiotemporally controlled, non-invasive genome engineering in living systems.
Thus, the FISC system expands the optogenetic toolbox for DNA recombination to achieve spatiotemporally controlled, non-invasive genome engineering in living systems.
The FISC system expands the optogenetic toolbox for DNA recombination to enable spatiotemporally controlled, non-invasive genome engineering in living systems.
Thus, the FISC system expands the optogenetic toolbox for DNA recombination to achieve spatiotemporally controlled, non-invasive genome engineering in living systems.
The FISC system expands the optogenetic toolbox for DNA recombination to enable spatiotemporally controlled, non-invasive genome engineering in living systems.
Thus, the FISC system expands the optogenetic toolbox for DNA recombination to achieve spatiotemporally controlled, non-invasive genome engineering in living systems.
The FISC system expands the optogenetic toolbox for DNA recombination to enable spatiotemporally controlled, non-invasive genome engineering in living systems.
Thus, the FISC system expands the optogenetic toolbox for DNA recombination to achieve spatiotemporally controlled, non-invasive genome engineering in living systems.
The FISC system expands the optogenetic toolbox for DNA recombination to enable spatiotemporally controlled, non-invasive genome engineering in living systems.
Thus, the FISC system expands the optogenetic toolbox for DNA recombination to achieve spatiotemporally controlled, non-invasive genome engineering in living systems.
The FISC system expands the optogenetic toolbox for DNA recombination to enable spatiotemporally controlled, non-invasive genome engineering in living systems.
Thus, the FISC system expands the optogenetic toolbox for DNA recombination to achieve spatiotemporally controlled, non-invasive genome engineering in living systems.
In vivo, the FISC system shows strong organ penetration and markedly outperforms two blue-light-based Cre systems for recombination induction in the liver.
Our in vivo studies showcase the strong organ-penetration capacity of FISC system, markedly outperforming two blue-light-based Cre systems for recombination induction in the liver.
In vivo, the FISC system shows strong organ penetration and markedly outperforms two blue-light-based Cre systems for recombination induction in the liver.
Our in vivo studies showcase the strong organ-penetration capacity of FISC system, markedly outperforming two blue-light-based Cre systems for recombination induction in the liver.
In vivo, the FISC system shows strong organ penetration and markedly outperforms two blue-light-based Cre systems for recombination induction in the liver.
Our in vivo studies showcase the strong organ-penetration capacity of FISC system, markedly outperforming two blue-light-based Cre systems for recombination induction in the liver.
In vivo, the FISC system shows strong organ penetration and markedly outperforms two blue-light-based Cre systems for recombination induction in the liver.
Our in vivo studies showcase the strong organ-penetration capacity of FISC system, markedly outperforming two blue-light-based Cre systems for recombination induction in the liver.
In vivo, the FISC system shows strong organ penetration and markedly outperforms two blue-light-based Cre systems for recombination induction in the liver.
Our in vivo studies showcase the strong organ-penetration capacity of FISC system, markedly outperforming two blue-light-based Cre systems for recombination induction in the liver.
In vivo, the FISC system shows strong organ penetration and markedly outperforms two blue-light-based Cre systems for recombination induction in the liver.
Our in vivo studies showcase the strong organ-penetration capacity of FISC system, markedly outperforming two blue-light-based Cre systems for recombination induction in the liver.
In vivo, the FISC system shows strong organ penetration and markedly outperforms two blue-light-based Cre systems for recombination induction in the liver.
Our in vivo studies showcase the strong organ-penetration capacity of FISC system, markedly outperforming two blue-light-based Cre systems for recombination induction in the liver.
In vivo, the FISC system shows strong organ penetration and markedly outperforms two blue-light-based Cre systems for recombination induction in the liver.
Our in vivo studies showcase the strong organ-penetration capacity of FISC system, markedly outperforming two blue-light-based Cre systems for recombination induction in the liver.
In vivo, the FISC system shows strong organ penetration and markedly outperforms two blue-light-based Cre systems for recombination induction in the liver.
Our in vivo studies showcase the strong organ-penetration capacity of FISC system, markedly outperforming two blue-light-based Cre systems for recombination induction in the liver.
In vivo, the FISC system shows strong organ penetration and markedly outperforms two blue-light-based Cre systems for recombination induction in the liver.
Our in vivo studies showcase the strong organ-penetration capacity of FISC system, markedly outperforming two blue-light-based Cre systems for recombination induction in the liver.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
The paper reports development of a far-red light-induced split Cre-loxP system called FISC for optogenetic regulation of genome engineering in vivo using far-red light.
Here, we develop a far-red light-induced split Cre-loxP system (FISC system) based on a bacteriophytochrome optogenetic system and split-Cre recombinase, enabling optogenetical regulation of genome engineering in vivo solely by utilizing a far-red light (FRL).
The paper reports development of a far-red light-induced split Cre-loxP system called FISC for optogenetic regulation of genome engineering in vivo using far-red light.
Here, we develop a far-red light-induced split Cre-loxP system (FISC system) based on a bacteriophytochrome optogenetic system and split-Cre recombinase, enabling optogenetical regulation of genome engineering in vivo solely by utilizing a far-red light (FRL).
The paper reports development of a far-red light-induced split Cre-loxP system called FISC for optogenetic regulation of genome engineering in vivo using far-red light.
Here, we develop a far-red light-induced split Cre-loxP system (FISC system) based on a bacteriophytochrome optogenetic system and split-Cre recombinase, enabling optogenetical regulation of genome engineering in vivo solely by utilizing a far-red light (FRL).
The paper reports development of a far-red light-induced split Cre-loxP system called FISC for optogenetic regulation of genome engineering in vivo using far-red light.
Here, we develop a far-red light-induced split Cre-loxP system (FISC system) based on a bacteriophytochrome optogenetic system and split-Cre recombinase, enabling optogenetical regulation of genome engineering in vivo solely by utilizing a far-red light (FRL).
The paper reports development of a far-red light-induced split Cre-loxP system called FISC for optogenetic regulation of genome engineering in vivo using far-red light.
Here, we develop a far-red light-induced split Cre-loxP system (FISC system) based on a bacteriophytochrome optogenetic system and split-Cre recombinase, enabling optogenetical regulation of genome engineering in vivo solely by utilizing a far-red light (FRL).
The paper reports development of a far-red light-induced split Cre-loxP system called FISC for optogenetic regulation of genome engineering in vivo using far-red light.
Here, we develop a far-red light-induced split Cre-loxP system (FISC system) based on a bacteriophytochrome optogenetic system and split-Cre recombinase, enabling optogenetical regulation of genome engineering in vivo solely by utilizing a far-red light (FRL).
The paper reports development of a far-red light-induced split Cre-loxP system called FISC for optogenetic regulation of genome engineering in vivo using far-red light.
Here, we develop a far-red light-induced split Cre-loxP system (FISC system) based on a bacteriophytochrome optogenetic system and split-Cre recombinase, enabling optogenetical regulation of genome engineering in vivo solely by utilizing a far-red light (FRL).
The paper reports development of a far-red light-induced split Cre-loxP system called FISC for optogenetic regulation of genome engineering in vivo using far-red light.
Here, we develop a far-red light-induced split Cre-loxP system (FISC system) based on a bacteriophytochrome optogenetic system and split-Cre recombinase, enabling optogenetical regulation of genome engineering in vivo solely by utilizing a far-red light (FRL).
The paper reports development of a far-red light-induced split Cre-loxP system called FISC for optogenetic regulation of genome engineering in vivo using far-red light.
Here, we develop a far-red light-induced split Cre-loxP system (FISC system) based on a bacteriophytochrome optogenetic system and split-Cre recombinase, enabling optogenetical regulation of genome engineering in vivo solely by utilizing a far-red light (FRL).
The paper reports development of a far-red light-induced split Cre-loxP system called FISC for optogenetic regulation of genome engineering in vivo using far-red light.
Here, we develop a far-red light-induced split Cre-loxP system (FISC system) based on a bacteriophytochrome optogenetic system and split-Cre recombinase, enabling optogenetical regulation of genome engineering in vivo solely by utilizing a far-red light (FRL).
The FISC system exhibits low background, no detectable photocytotoxicity, and efficient far-red-light-induced DNA recombination.
The FISC system exhibits low background and no detectable photocytotoxicity, while offering efficient FRL-induced DNA recombination.
The FISC system exhibits low background, no detectable photocytotoxicity, and efficient far-red-light-induced DNA recombination.
The FISC system exhibits low background and no detectable photocytotoxicity, while offering efficient FRL-induced DNA recombination.
The FISC system exhibits low background, no detectable photocytotoxicity, and efficient far-red-light-induced DNA recombination.
The FISC system exhibits low background and no detectable photocytotoxicity, while offering efficient FRL-induced DNA recombination.
The FISC system exhibits low background, no detectable photocytotoxicity, and efficient far-red-light-induced DNA recombination.
The FISC system exhibits low background and no detectable photocytotoxicity, while offering efficient FRL-induced DNA recombination.
The FISC system exhibits low background, no detectable photocytotoxicity, and efficient far-red-light-induced DNA recombination.
The FISC system exhibits low background and no detectable photocytotoxicity, while offering efficient FRL-induced DNA recombination.
The FISC system exhibits low background, no detectable photocytotoxicity, and efficient far-red-light-induced DNA recombination.
The FISC system exhibits low background and no detectable photocytotoxicity, while offering efficient FRL-induced DNA recombination.
The FISC system exhibits low background, no detectable photocytotoxicity, and efficient far-red-light-induced DNA recombination.
The FISC system exhibits low background and no detectable photocytotoxicity, while offering efficient FRL-induced DNA recombination.
The FISC system exhibits low background, no detectable photocytotoxicity, and efficient far-red-light-induced DNA recombination.
The FISC system exhibits low background and no detectable photocytotoxicity, while offering efficient FRL-induced DNA recombination.
The FISC system exhibits low background, no detectable photocytotoxicity, and efficient far-red-light-induced DNA recombination.
The FISC system exhibits low background and no detectable photocytotoxicity, while offering efficient FRL-induced DNA recombination.
The FISC system exhibits low background, no detectable photocytotoxicity, and efficient far-red-light-induced DNA recombination.
The FISC system exhibits low background and no detectable photocytotoxicity, while offering efficient FRL-induced DNA recombination.
Approval Evidence
1 source1 linked approval claimfirst-pass slug adeno-associated-virus-delivery
we successfully deploy a FISC system using adeno-associated virus (AAV) delivery
Source:
delivery applicationsupports
The FISC system was successfully deployed using adeno-associated virus delivery.
Demonstrating its strong clinical relevance, we successfully deploy a FISC system using adeno-associated virus (AAV) delivery.
Source:
Comparisons
Source-backed strengths
The main demonstrated strength is successful in vivo deployment of the FISC system using AAV delivery. Because the associated FISC platform enabled non-invasive genome engineering and showed strong organ penetration with superior liver recombination induction relative to two blue-light-based Cre systems, AAV delivery was compatible with that validated in vivo application.
Compared with AAV-PA-Cre 3.0
adeno-associated virus delivery and AAV-PA-Cre 3.0 address a similar problem space.
Shared frame: same top-level item type
Compared with HiRet
adeno-associated virus delivery and HiRet address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Ranked Citations
- 1.