Source 1primary paper2025MED
Toolkit/AlphaFold3
AlphaFold3
Also known as: AlphaFold 3, AlphaFold3
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
AlphaFold3 is a computational structure-prediction method used in the cited study to model the MagMboI–DNA complex. In that work, it was applied to infer interactions with the 5'-GATC-3' recognition sequence and to guide optimization of the photoactivatable endonuclease variant MagMboI-plus for top-down genome engineering.
Usefulness & Problems
Why this is useful
In the cited study, AlphaFold3 was useful for generating a structural model of a protein–DNA complex that informed interpretation of sequence recognition by MagMboI. This enabled structure-guided optimization of a photoactivatable nuclease in the absence of other evidence described here.
Problem solved
The specific problem addressed in the cited work was how to rationalize MagMboI recognition of the 5'-GATC-3' DNA sequence and use that information to improve a photoactivatable endonuclease variant. The available evidence does not provide broader benchmarking or general performance claims for AlphaFold3 beyond this application.
Problem links
Need precise spatiotemporal control with light input
DerivedAlphaFold3 is a computational structure-prediction method used here to model the MagMboI–DNA complex and infer interactions with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage. In the cited study, it guided optimization of a photoactivatable endonuclease variant, MagMboI-plus, for top-down genome engineering.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete computational method used to design, rank, or analyze an engineered system.
Mechanisms
Photocleavagestructural modeling of protein–dna complexesstructural modeling of protein–dna complexesstructural modeling of protein–dna complexesTarget processes
editingInput: Light
Implementation Constraints
ai structure prediction: Truealgorithm family: deep learning structure predictioncofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementmaturity: emergingoperating role: builderswitch architecture: cleavageswitch architecture: multi componentswitch architecture: recruitmentused for protein dna modeling: True
The reported implementation involved modeling a MagMboI–DNA complex containing the 5'-GATC-3' recognition sequence to infer interactions relevant to Mg2+-dependent cleavage. The downstream biological validation was performed in Saccharomyces cerevisiae using a photoactivatable endonuclease system, but no additional practical details on software setup or input requirements are provided in the supplied evidence.
The supplied evidence is limited to a single 2025 study and one application context involving MagMboI optimization. No independent replication, quantitative accuracy metrics, runtime information, or comparisons to alternative structure-prediction methods are provided here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2primary paper2026MED
Source 3review2025MED
Ranked Claims
Next-generation countermeasures for Bt resistance include synergistic Cry/Vip pyramiding, CRISPR/Cas9-validated receptor knockouts revealing functional redundancy, Domain III chimerization, PACE, and AlphaFold3-guided rational redesign.
Countermeasures now integrate synergistic Cry/Vip pyramiding, CRISPR/Cas9-validated receptor knockouts revealing functional redundancy, Domain III chimerization (e.g., Cry1A.105), phage-assisted continuous evolution (PACE), and the emerging application of AlphaFold3 for structure-guided rational redesign of resistance-breaking variants.
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
MagMboI-plus induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
genomic rearrangements more pronounced
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
In Saccharomyces cerevisiae cells, MagMboI-plus showed slightly increased DNA-cleavage activity in vivo upon blue light activation compared with the original MagMboI construct.
DNA-cleavage activity change slightly increased
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
MagMboI functions through a split-protein strategy in which blue-light-induced heterodimerization restores nuclease activity.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
MagMboI is a photoactivatable restriction enzyme designed for light-controlled top-down genome engineering.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
An alternative split-site variant, MagMboI-plus, increases the MagMboI-DNA interface area and enhances complex stability relative to the original construct.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
MagMboI-plus preserves alpha-helical integrity while strengthening protein-DNA contacts.
Approval Evidence
4 sources4 linked approval claimsfirst-pass slugs alphafold-3, alphafold3, alphafold3-guided-rational-redesign
the emerging application of AlphaFold3 for structure-guided rational redesign of resistance-breaking variants
Source:
We predicted 3D structures ... using three popular RNA 3D modeling tools, namely RNAComposer, FARFAR2, and AlphaFold3
Source:
Using AlphaFold3, we modeled the structure of the MagMboI-DNA complex and gained structural insights into the interaction between MagMboI and its target DNA recognition sequence (5'-GATC-3') required for Mg2+-dependent DNA cleavage.
Source:
We discuss the complementary roles of cryo-EM and AI, including developments in direct electron detectors, advanced image processing, and deep learning algorithms exemplified by AlphaFold 2 and the emerging AlphaFold 3.
Source:
engineering strategysupports
Next-generation countermeasures for Bt resistance include synergistic Cry/Vip pyramiding, CRISPR/Cas9-validated receptor knockouts revealing functional redundancy, Domain III chimerization, PACE, and AlphaFold3-guided rational redesign.
Countermeasures now integrate synergistic Cry/Vip pyramiding, CRISPR/Cas9-validated receptor knockouts revealing functional redundancy, Domain III chimerization (e.g., Cry1A.105), phage-assisted continuous evolution (PACE), and the emerging application of AlphaFold3 for structure-guided rational redesign of resistance-breaking variants.
Source:
method compositionsupports
Candidate RNA 3D conformations were generated using RNAComposer, FARFAR2, and AlphaFold3.
Source:
general strategysupports
AlphaFold3-based prediction can accelerate functional improvements in engineered enzymes and provide a strategy for developing light-controlled genome engineering tools.
Source:
structure guided designsupports
AlphaFold3 was used to model the MagMboI-DNA complex and provide structural insight into interaction with the 5'-GATC-3' recognition sequence required for Mg2+-dependent DNA cleavage.
Source:
Comparisons
Source-backed strengths
The cited study reports that AlphaFold3 supported modeling of the MagMboI–DNA complex and inference of contacts relevant to Mg2+-dependent DNA cleavage. In the resulting application, the AlphaFold3-guided MagMboI-plus construct induced more pronounced genomic rearrangements than the original MagMboI construct in Saccharomyces cerevisiae cells.
Compared with alkynyl-functionalized photocleavable linker
AlphaFold3 and alkynyl-functionalized photocleavable linker address a similar problem space.
Shared frame: shared mechanisms: photocleavage; same primary input modality: light
Compared with GFP-PHR-caspase8/Flag-CIB1N-caspase8
AlphaFold3 and GFP-PHR-caspase8/Flag-CIB1N-caspase8 address a similar problem space.
Shared frame: shared mechanisms: photocleavage; same primary input modality: light
Strengths here: looks easier to implement in practice.
Compared with light-responsive nano-regulators
AlphaFold3 and light-responsive nano-regulators address a similar problem space.
Shared frame: shared mechanisms: photocleavage; same primary input modality: light
Ranked Citations
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Extracted from this source document.