Source 1primary paper2025PPR
Toolkit/Anchor-Away
Anchor-Away
Also known as: AA, rapamycin-based anchor-away
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The anchor-away (AA) technique enables rapid depletion of nuclear proteins by tethering them to cytoplasmic anchors through rapamycin-induced heterodimerisation.
Usefulness & Problems
No literature-backed usefulness or problem-fit explainer has been materialized for this record yet.
Published Workflows
A simple, reversible and non-toxic anchor-away system for effective nuclear depletion of proteins
2025Objective: Develop an alternative anchor-away system for nuclear protein depletion that avoids rapamycin-associated toxicity and irreversibility while preserving effective depletion.
Why it works: The workflow replaces rapamycin-induced target-anchor association with ABA-induced association so that nuclear depletion can still be achieved while avoiding rapamycin-linked TOR signaling and heat-shock confounds.
conditional association of a nuclear target to a cytoplasmic anchorinducer-controlled nuclear depletion by relocalizationanchor-away system redesignsmall-molecule-induced heterodimerization replacement
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
chemically induced sequestrationHeterodimerizationinduced relocalization of nuclear proteins to cytoplasmic anchorsTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Anchor-Away enables rapid depletion of nuclear proteins by tethering them to cytoplasmic anchors through rapamycin-induced heterodimerisation.
Unlike rapamycin, abscisic acid does not cause major gene expression changes and is suitable for diverse genetic backgrounds in this anchor-away context.
The authors developed an alternative anchor-away system that uses abscisic acid to induce conditional association of the target to its cytoplasmic anchor.
In Saccharomyces cerevisiae, rapamycin-based Anchor-Away is restricted to rapamycin-resistant strains because rapamycin inhibits TOR signalling and hinders the heat-shock response.
The rapamycin-based Anchor-Away method is not fully reversible, limiting studies of dynamic cellular processes that require transient perturbation and functional restoration.
The ABA-AA system enables efficient and fully reversible depletion of highly abundant nuclear proteins.
The ABA-AA system is presented as a fully reversible, non-toxic, and broadly applicable alternative for nuclear protein depletion across eukaryotic systems.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug anchor-away
The anchor-away (AA) technique enables rapid depletion of nuclear proteins by tethering them to cytoplasmic anchors through rapamycin-induced heterodimerisation.
Source:
capabilitysupports
Anchor-Away enables rapid depletion of nuclear proteins by tethering them to cytoplasmic anchors through rapamycin-induced heterodimerisation.
Source:
limitationsupports
In Saccharomyces cerevisiae, rapamycin-based Anchor-Away is restricted to rapamycin-resistant strains because rapamycin inhibits TOR signalling and hinders the heat-shock response.
Source:
limitationsupports
The rapamycin-based Anchor-Away method is not fully reversible, limiting studies of dynamic cellular processes that require transient perturbation and functional restoration.
Source:
Comparisons
No literature-backed comparison notes have been materialized for this record yet.
Ranked Citations
- 1.