Source 1primary paper2013Biochemistry
Toolkit/AQTrip EL222 variant
AQTrip EL222 variant
Also known as: AQTrip
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
AQTrip is an engineered EL222 variant carrying V41I, L52I, A79Q, and V121I substitutions in the blue-light-responsive LOV–HTH transcription factor. It stabilizes the photoactivated state and, in the reported study, oligomerizes without DNA and forms an EL222 dimer–DNA complex in the presence of DNA substrates.
Usefulness & Problems
Why this is useful
AQTrip is useful as a light-responsive protein switch because it prolongs the activated state of EL222 and supports DNA-associated dimer formation under blue-light-responsive control. This makes it relevant for applications that require coupling light input to EL222 DNA-binding behavior and transcription-related regulation.
Problem solved
This variant addresses the problem of limited stability of the EL222 photoactivated state by introducing four point mutations that stabilize that state. The reported behavior also provides a defined DNA-dependent assembly mode, with oligomerization in the absence of DNA and dimer–DNA complex formation when DNA substrates are present.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
recombinationInput: Light
Implementation Constraints
AQTrip is implemented by site-specific substitution of EL222 at V41I, L52I, A79Q, and V121I. The input modality is blue light, and the parent EL222 system operates through reorientation of LOV sensory and HTH effector domains to enable photoactivation of gene transcription.
The available evidence is limited to a single cited study and does not provide quantitative performance metrics in the supplied material. The evidence also does not establish independent replication, detailed cellular validation, or direct demonstration in recombination applications from the provided text.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
AQTrip oligomerizes in the absence of DNA and forms an EL222 dimer-DNA complex in the presence of DNA substrates.
Size-exclusion chromatography and light scattering indicate that AQTrip oligomerizes in the absence of DNA and selects for an EL222 dimer-DNA complex in the presence of DNA substrates
AQTrip oligomerizes in the absence of DNA and forms an EL222 dimer-DNA complex in the presence of DNA substrates.
Size-exclusion chromatography and light scattering indicate that AQTrip oligomerizes in the absence of DNA and selects for an EL222 dimer-DNA complex in the presence of DNA substrates
AQTrip oligomerizes in the absence of DNA and forms an EL222 dimer-DNA complex in the presence of DNA substrates.
Size-exclusion chromatography and light scattering indicate that AQTrip oligomerizes in the absence of DNA and selects for an EL222 dimer-DNA complex in the presence of DNA substrates
AQTrip oligomerizes in the absence of DNA and forms an EL222 dimer-DNA complex in the presence of DNA substrates.
Size-exclusion chromatography and light scattering indicate that AQTrip oligomerizes in the absence of DNA and selects for an EL222 dimer-DNA complex in the presence of DNA substrates
AQTrip oligomerizes in the absence of DNA and forms an EL222 dimer-DNA complex in the presence of DNA substrates.
Size-exclusion chromatography and light scattering indicate that AQTrip oligomerizes in the absence of DNA and selects for an EL222 dimer-DNA complex in the presence of DNA substrates
AQTrip oligomerizes in the absence of DNA and forms an EL222 dimer-DNA complex in the presence of DNA substrates.
Size-exclusion chromatography and light scattering indicate that AQTrip oligomerizes in the absence of DNA and selects for an EL222 dimer-DNA complex in the presence of DNA substrates
AQTrip oligomerizes in the absence of DNA and forms an EL222 dimer-DNA complex in the presence of DNA substrates.
Size-exclusion chromatography and light scattering indicate that AQTrip oligomerizes in the absence of DNA and selects for an EL222 dimer-DNA complex in the presence of DNA substrates
The AQTrip EL222 variant stabilizes the photoactivated state.
creating an EL222 variant harboring V41I, L52I, A79Q, and V121I point mutations (AQTrip) that stabilizes the photoactivated state
The AQTrip EL222 variant stabilizes the photoactivated state.
creating an EL222 variant harboring V41I, L52I, A79Q, and V121I point mutations (AQTrip) that stabilizes the photoactivated state
The AQTrip EL222 variant stabilizes the photoactivated state.
creating an EL222 variant harboring V41I, L52I, A79Q, and V121I point mutations (AQTrip) that stabilizes the photoactivated state
The AQTrip EL222 variant stabilizes the photoactivated state.
creating an EL222 variant harboring V41I, L52I, A79Q, and V121I point mutations (AQTrip) that stabilizes the photoactivated state
The AQTrip EL222 variant stabilizes the photoactivated state.
creating an EL222 variant harboring V41I, L52I, A79Q, and V121I point mutations (AQTrip) that stabilizes the photoactivated state
The AQTrip EL222 variant stabilizes the photoactivated state.
creating an EL222 variant harboring V41I, L52I, A79Q, and V121I point mutations (AQTrip) that stabilizes the photoactivated state
The AQTrip EL222 variant stabilizes the photoactivated state.
creating an EL222 variant harboring V41I, L52I, A79Q, and V121I point mutations (AQTrip) that stabilizes the photoactivated state
EL222 uses blue light to drive reorientation of LOV sensory and HTH effector domains, allowing photoactivation of gene transcription.
it harnesses blue light to drive the reorientation of light-oxygen-voltage (LOV) sensory and helix-turn-helix (HTH) effector domains to allow photoactivation of gene transcription in natural and artificial systems
EL222 uses blue light to drive reorientation of LOV sensory and HTH effector domains, allowing photoactivation of gene transcription.
it harnesses blue light to drive the reorientation of light-oxygen-voltage (LOV) sensory and helix-turn-helix (HTH) effector domains to allow photoactivation of gene transcription in natural and artificial systems
EL222 uses blue light to drive reorientation of LOV sensory and HTH effector domains, allowing photoactivation of gene transcription.
it harnesses blue light to drive the reorientation of light-oxygen-voltage (LOV) sensory and helix-turn-helix (HTH) effector domains to allow photoactivation of gene transcription in natural and artificial systems
EL222 uses blue light to drive reorientation of LOV sensory and HTH effector domains, allowing photoactivation of gene transcription.
it harnesses blue light to drive the reorientation of light-oxygen-voltage (LOV) sensory and helix-turn-helix (HTH) effector domains to allow photoactivation of gene transcription in natural and artificial systems
EL222 uses blue light to drive reorientation of LOV sensory and HTH effector domains, allowing photoactivation of gene transcription.
it harnesses blue light to drive the reorientation of light-oxygen-voltage (LOV) sensory and helix-turn-helix (HTH) effector domains to allow photoactivation of gene transcription in natural and artificial systems
EL222 uses blue light to drive reorientation of LOV sensory and HTH effector domains, allowing photoactivation of gene transcription.
it harnesses blue light to drive the reorientation of light-oxygen-voltage (LOV) sensory and helix-turn-helix (HTH) effector domains to allow photoactivation of gene transcription in natural and artificial systems
EL222 uses blue light to drive reorientation of LOV sensory and HTH effector domains, allowing photoactivation of gene transcription.
it harnesses blue light to drive the reorientation of light-oxygen-voltage (LOV) sensory and helix-turn-helix (HTH) effector domains to allow photoactivation of gene transcription in natural and artificial systems
Blue light induces EL222 dimerization through LOV and HTH interfaces.
Combined, these novel approaches have validated a key mechanistic step, whereby blue light induces EL222 dimerization through LOV and HTH interfaces
Blue light induces EL222 dimerization through LOV and HTH interfaces.
Combined, these novel approaches have validated a key mechanistic step, whereby blue light induces EL222 dimerization through LOV and HTH interfaces
Blue light induces EL222 dimerization through LOV and HTH interfaces.
Combined, these novel approaches have validated a key mechanistic step, whereby blue light induces EL222 dimerization through LOV and HTH interfaces
Blue light induces EL222 dimerization through LOV and HTH interfaces.
Combined, these novel approaches have validated a key mechanistic step, whereby blue light induces EL222 dimerization through LOV and HTH interfaces
Blue light induces EL222 dimerization through LOV and HTH interfaces.
Combined, these novel approaches have validated a key mechanistic step, whereby blue light induces EL222 dimerization through LOV and HTH interfaces
Blue light induces EL222 dimerization through LOV and HTH interfaces.
Combined, these novel approaches have validated a key mechanistic step, whereby blue light induces EL222 dimerization through LOV and HTH interfaces
Blue light induces EL222 dimerization through LOV and HTH interfaces.
Combined, these novel approaches have validated a key mechanistic step, whereby blue light induces EL222 dimerization through LOV and HTH interfaces
The EL222-DNA complex has a 2:1 stoichiometry with a previously characterized DNA substrate.
NMR analyses of the EL222-DNA complex confirm a 2:1 stoichiometry in the presence of a previously characterized DNA substrate
complex stoichiometry 2:1
The EL222-DNA complex has a 2:1 stoichiometry with a previously characterized DNA substrate.
NMR analyses of the EL222-DNA complex confirm a 2:1 stoichiometry in the presence of a previously characterized DNA substrate
complex stoichiometry 2:1
The EL222-DNA complex has a 2:1 stoichiometry with a previously characterized DNA substrate.
NMR analyses of the EL222-DNA complex confirm a 2:1 stoichiometry in the presence of a previously characterized DNA substrate
complex stoichiometry 2:1
The EL222-DNA complex has a 2:1 stoichiometry with a previously characterized DNA substrate.
NMR analyses of the EL222-DNA complex confirm a 2:1 stoichiometry in the presence of a previously characterized DNA substrate
complex stoichiometry 2:1
The EL222-DNA complex has a 2:1 stoichiometry with a previously characterized DNA substrate.
NMR analyses of the EL222-DNA complex confirm a 2:1 stoichiometry in the presence of a previously characterized DNA substrate
complex stoichiometry 2:1
The EL222-DNA complex has a 2:1 stoichiometry with a previously characterized DNA substrate.
NMR analyses of the EL222-DNA complex confirm a 2:1 stoichiometry in the presence of a previously characterized DNA substrate
complex stoichiometry 2:1
The EL222-DNA complex has a 2:1 stoichiometry with a previously characterized DNA substrate.
NMR analyses of the EL222-DNA complex confirm a 2:1 stoichiometry in the presence of a previously characterized DNA substrate
complex stoichiometry 2:1
Approval Evidence
1 source2 linked approval claimsfirst-pass slug aqtrip-el222-variant
creating an EL222 variant harboring V41I, L52I, A79Q, and V121I point mutations (AQTrip) that stabilizes the photoactivated state
Source:
biophysical behaviorsupports
AQTrip oligomerizes in the absence of DNA and forms an EL222 dimer-DNA complex in the presence of DNA substrates.
Size-exclusion chromatography and light scattering indicate that AQTrip oligomerizes in the absence of DNA and selects for an EL222 dimer-DNA complex in the presence of DNA substrates
Source:
engineering resultsupports
The AQTrip EL222 variant stabilizes the photoactivated state.
creating an EL222 variant harboring V41I, L52I, A79Q, and V121I point mutations (AQTrip) that stabilizes the photoactivated state
Source:
Comparisons
Source-backed strengths
The key reported strength is stabilization of the EL222 photoactivated state in a defined four-mutation variant. The study also directly reports distinct assembly behaviors depending on DNA context, namely oligomerization without DNA and dimer–DNA complex formation with DNA substrates.
Source:
creating an EL222 variant harboring V41I, L52I, A79Q, and V121I point mutations (AQTrip) that stabilizes the photoactivated state
Ranked Citations
- 1.