Source 1primary paper2017
Toolkit/ArrayG
ArrayG
Also known as: fluorogenic ArrayG tag
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
ArrayG is a fluorogenic nanobody array tag built from linear repeats of GFP nanobodies that recruit free monomeric wild-type GFP. GFP fluorescence increases by approximately 15-fold upon array binding, enabling prolonged live-cell single-molecule imaging.
Usefulness & Problems
Why this is useful
ArrayG is useful for long-duration single-molecule tracking because its fluorogenic behavior reduces background while supporting sustained visualization of labeled targets in live cells. Source literature reports continuous tracking of single integrins for up to 105 seconds or 2100 frames and detection of repeated state-switching events for kinesin and integrin.
Source:
We also report an orthogonal array tag, based on a DHFR-nanobody, for prolonged dual color imaging of single molecules.
Source:
The photo-stability of ArrayG and consistently low background made it possible to continuously track single integrins for as long as 105 seconds (2100 frames).
Problem solved
ArrayG addresses the problem of limited single-molecule imaging duration caused by background fluorescence and insufficient photostability in live cells. The tool provides a way to recruit fluorescent GFP to a multivalent tag and increase signal upon binding, improving trackability of individual molecules over extended time courses.
Source:
We also report an orthogonal array tag, based on a DHFR-nanobody, for prolonged dual color imaging of single molecules.
Source:
The photo-stability of ArrayG and consistently low background made it possible to continuously track single integrins for as long as 105 seconds (2100 frames).
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
localizationrecombinationInput: Light
Implementation Constraints
ArrayG consists of a linear repeat array of GFP nanobodies and requires co-availability of free monomeric wild-type GFP as the recruited fluorogenic binder. The provided evidence supports use in live-cell single-molecule imaging, but it does not specify construct architecture, repeat number, expression system, or delivery method.
The supplied evidence is limited to a single 2017 source and focuses on imaging performance rather than broad benchmarking across targets, cell types, or experimental formats. The evidence also mentions an orthogonal DHFR-nanobody array for dual-color imaging, but no detailed performance data for ArrayG beyond the reported applications are provided here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
An orthogonal array tag based on a DHFR-nanobody was reported for prolonged dual color imaging of single molecules.
We also report an orthogonal array tag, based on a DHFR-nanobody, for prolonged dual color imaging of single molecules.
An orthogonal array tag based on a DHFR-nanobody was reported for prolonged dual color imaging of single molecules.
We also report an orthogonal array tag, based on a DHFR-nanobody, for prolonged dual color imaging of single molecules.
An orthogonal array tag based on a DHFR-nanobody was reported for prolonged dual color imaging of single molecules.
We also report an orthogonal array tag, based on a DHFR-nanobody, for prolonged dual color imaging of single molecules.
An orthogonal array tag based on a DHFR-nanobody was reported for prolonged dual color imaging of single molecules.
We also report an orthogonal array tag, based on a DHFR-nanobody, for prolonged dual color imaging of single molecules.
An orthogonal array tag based on a DHFR-nanobody was reported for prolonged dual color imaging of single molecules.
We also report an orthogonal array tag, based on a DHFR-nanobody, for prolonged dual color imaging of single molecules.
An orthogonal array tag based on a DHFR-nanobody was reported for prolonged dual color imaging of single molecules.
We also report an orthogonal array tag, based on a DHFR-nanobody, for prolonged dual color imaging of single molecules.
An orthogonal array tag based on a DHFR-nanobody was reported for prolonged dual color imaging of single molecules.
We also report an orthogonal array tag, based on a DHFR-nanobody, for prolonged dual color imaging of single molecules.
ArrayG photo-stability and low background enabled continuous tracking of single integrins for up to 105 seconds or 2100 frames.
The photo-stability of ArrayG and consistently low background made it possible to continuously track single integrins for as long as 105 seconds (2100 frames).
continuous tracking duration 105 secondscontinuous tracking frames 2100 frames
ArrayG photo-stability and low background enabled continuous tracking of single integrins for up to 105 seconds or 2100 frames.
The photo-stability of ArrayG and consistently low background made it possible to continuously track single integrins for as long as 105 seconds (2100 frames).
continuous tracking duration 105 secondscontinuous tracking frames 2100 frames
ArrayG photo-stability and low background enabled continuous tracking of single integrins for up to 105 seconds or 2100 frames.
The photo-stability of ArrayG and consistently low background made it possible to continuously track single integrins for as long as 105 seconds (2100 frames).
continuous tracking duration 105 secondscontinuous tracking frames 2100 frames
ArrayG photo-stability and low background enabled continuous tracking of single integrins for up to 105 seconds or 2100 frames.
The photo-stability of ArrayG and consistently low background made it possible to continuously track single integrins for as long as 105 seconds (2100 frames).
continuous tracking duration 105 secondscontinuous tracking frames 2100 frames
ArrayG photo-stability and low background enabled continuous tracking of single integrins for up to 105 seconds or 2100 frames.
The photo-stability of ArrayG and consistently low background made it possible to continuously track single integrins for as long as 105 seconds (2100 frames).
continuous tracking duration 105 secondscontinuous tracking frames 2100 frames
ArrayG photo-stability and low background enabled continuous tracking of single integrins for up to 105 seconds or 2100 frames.
The photo-stability of ArrayG and consistently low background made it possible to continuously track single integrins for as long as 105 seconds (2100 frames).
continuous tracking duration 105 secondscontinuous tracking frames 2100 frames
ArrayG photo-stability and low background enabled continuous tracking of single integrins for up to 105 seconds or 2100 frames.
The photo-stability of ArrayG and consistently low background made it possible to continuously track single integrins for as long as 105 seconds (2100 frames).
continuous tracking duration 105 secondscontinuous tracking frames 2100 frames
Prolonged tracking of kinesin and integrin revealed repeated state-switching events.
Prolonged tracking of both kinesin and integrin revealed repeated state-switching events, a measurement capability that is crucial to a mechanistic understanding of complex cellular processes.
Prolonged tracking of kinesin and integrin revealed repeated state-switching events.
Prolonged tracking of both kinesin and integrin revealed repeated state-switching events, a measurement capability that is crucial to a mechanistic understanding of complex cellular processes.
Prolonged tracking of kinesin and integrin revealed repeated state-switching events.
Prolonged tracking of both kinesin and integrin revealed repeated state-switching events, a measurement capability that is crucial to a mechanistic understanding of complex cellular processes.
Prolonged tracking of kinesin and integrin revealed repeated state-switching events.
Prolonged tracking of both kinesin and integrin revealed repeated state-switching events, a measurement capability that is crucial to a mechanistic understanding of complex cellular processes.
Prolonged tracking of kinesin and integrin revealed repeated state-switching events.
Prolonged tracking of both kinesin and integrin revealed repeated state-switching events, a measurement capability that is crucial to a mechanistic understanding of complex cellular processes.
Prolonged tracking of kinesin and integrin revealed repeated state-switching events.
Prolonged tracking of both kinesin and integrin revealed repeated state-switching events, a measurement capability that is crucial to a mechanistic understanding of complex cellular processes.
Prolonged tracking of kinesin and integrin revealed repeated state-switching events.
Prolonged tracking of both kinesin and integrin revealed repeated state-switching events, a measurement capability that is crucial to a mechanistic understanding of complex cellular processes.
ArrayG is a linear repeat of GFP-nanobodies that recruits free monomeric wild-type GFP and increases GFP brightness upon binding.
ArrayG, a linear repeat of GFP-nanobodies, recruits free monomeric wild-type GFP, which brightens ~15-fold upon binding the array.
brightness increase upon binding ~15-fold
ArrayG is a linear repeat of GFP-nanobodies that recruits free monomeric wild-type GFP and increases GFP brightness upon binding.
ArrayG, a linear repeat of GFP-nanobodies, recruits free monomeric wild-type GFP, which brightens ~15-fold upon binding the array.
brightness increase upon binding ~15-fold
ArrayG is a linear repeat of GFP-nanobodies that recruits free monomeric wild-type GFP and increases GFP brightness upon binding.
ArrayG, a linear repeat of GFP-nanobodies, recruits free monomeric wild-type GFP, which brightens ~15-fold upon binding the array.
brightness increase upon binding ~15-fold
ArrayG is a linear repeat of GFP-nanobodies that recruits free monomeric wild-type GFP and increases GFP brightness upon binding.
ArrayG, a linear repeat of GFP-nanobodies, recruits free monomeric wild-type GFP, which brightens ~15-fold upon binding the array.
brightness increase upon binding ~15-fold
ArrayG is a linear repeat of GFP-nanobodies that recruits free monomeric wild-type GFP and increases GFP brightness upon binding.
ArrayG, a linear repeat of GFP-nanobodies, recruits free monomeric wild-type GFP, which brightens ~15-fold upon binding the array.
brightness increase upon binding ~15-fold
ArrayG is a linear repeat of GFP-nanobodies that recruits free monomeric wild-type GFP and increases GFP brightness upon binding.
ArrayG, a linear repeat of GFP-nanobodies, recruits free monomeric wild-type GFP, which brightens ~15-fold upon binding the array.
brightness increase upon binding ~15-fold
ArrayG is a linear repeat of GFP-nanobodies that recruits free monomeric wild-type GFP and increases GFP brightness upon binding.
ArrayG, a linear repeat of GFP-nanobodies, recruits free monomeric wild-type GFP, which brightens ~15-fold upon binding the array.
brightness increase upon binding ~15-fold
The fluorogenic ArrayG tag eliminates background fluorescence from free binders.
The fluorogenic ArrayG tag effectively eliminates background fluorescence from free binders, a major impediment to high-throughput acquisition of long trajectories in recruitment based imaging strategies.
The fluorogenic ArrayG tag eliminates background fluorescence from free binders.
The fluorogenic ArrayG tag effectively eliminates background fluorescence from free binders, a major impediment to high-throughput acquisition of long trajectories in recruitment based imaging strategies.
The fluorogenic ArrayG tag eliminates background fluorescence from free binders.
The fluorogenic ArrayG tag effectively eliminates background fluorescence from free binders, a major impediment to high-throughput acquisition of long trajectories in recruitment based imaging strategies.
The fluorogenic ArrayG tag eliminates background fluorescence from free binders.
The fluorogenic ArrayG tag effectively eliminates background fluorescence from free binders, a major impediment to high-throughput acquisition of long trajectories in recruitment based imaging strategies.
The fluorogenic ArrayG tag eliminates background fluorescence from free binders.
The fluorogenic ArrayG tag effectively eliminates background fluorescence from free binders, a major impediment to high-throughput acquisition of long trajectories in recruitment based imaging strategies.
The fluorogenic ArrayG tag eliminates background fluorescence from free binders.
The fluorogenic ArrayG tag effectively eliminates background fluorescence from free binders, a major impediment to high-throughput acquisition of long trajectories in recruitment based imaging strategies.
The fluorogenic ArrayG tag eliminates background fluorescence from free binders.
The fluorogenic ArrayG tag effectively eliminates background fluorescence from free binders, a major impediment to high-throughput acquisition of long trajectories in recruitment based imaging strategies.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug arrayg
ArrayG, a linear repeat of GFP-nanobodies, recruits free monomeric wild-type GFP, which brightens ~15-fold upon binding the array.
Source:
application capabilitysupports
ArrayG photo-stability and low background enabled continuous tracking of single integrins for up to 105 seconds or 2100 frames.
The photo-stability of ArrayG and consistently low background made it possible to continuously track single integrins for as long as 105 seconds (2100 frames).
Source:
measurement capabilitysupports
Prolonged tracking of kinesin and integrin revealed repeated state-switching events.
Prolonged tracking of both kinesin and integrin revealed repeated state-switching events, a measurement capability that is crucial to a mechanistic understanding of complex cellular processes.
Source:
molecular functionsupports
ArrayG is a linear repeat of GFP-nanobodies that recruits free monomeric wild-type GFP and increases GFP brightness upon binding.
ArrayG, a linear repeat of GFP-nanobodies, recruits free monomeric wild-type GFP, which brightens ~15-fold upon binding the array.
Source:
performance claimsupports
The fluorogenic ArrayG tag eliminates background fluorescence from free binders.
The fluorogenic ArrayG tag effectively eliminates background fluorescence from free binders, a major impediment to high-throughput acquisition of long trajectories in recruitment based imaging strategies.
Source:
Comparisons
Source-backed strengths
The reported strengths are fluorogenic brightening of wild-type GFP by about 15-fold on binding and sufficient photostability with low background for prolonged live-cell tracking. In the cited study, these properties enabled continuous tracking of single integrins for up to 105 seconds or 2100 frames and revealed repeated state-switching events in kinesin and integrin trajectories.
Source:
The fluorogenic ArrayG tag effectively eliminates background fluorescence from free binders, a major impediment to high-throughput acquisition of long trajectories in recruitment based imaging strategies.
Ranked Citations
- 1.