Source 1primary paper2019
Toolkit/aureochrome 1 LOV-domain-based optical TrkB activation approach
aureochrome 1 LOV-domain-based optical TrkB activation approach
Also known as: light-oxygen-voltage domain of aureochrome 1, optical TrkB activation based on the light-oxygen-voltage domain of aureochrome 1 from Vaucheria frigida
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The aureochrome 1 LOV-domain-based optical TrkB activation approach is an optogenetic TrkB activation strategy built around the light-oxygen-voltage domain of aureochrome 1 from Vaucheria frigida. It was presented as a demonstration that optical TrkB activation can be implemented with an optical homo-dimerizer other than CRY2.
Usefulness & Problems
Why this is useful
This approach is useful as evidence that optogenetic TrkB control is not restricted to CRY2-based designs. It expands the design space for light-controlled TrkB signaling by showing compatibility with an aureochrome 1 LOV-domain module.
Source:
Here we develop different optogenetic approaches that use light to activate TrkB receptors.
Source:
The optogenetic strategies presented are promising tools to investigate BDNF/TrkB signaling with tight spatial and temporal control.
Problem solved
It addresses the engineering problem of whether optical TrkB activation strategies are generalizable across different light-responsive homodimerizing modules. The cited evidence specifically positions this tool as an alternative implementation to CRY2-based optical TrkB systems.
Source:
Here we develop different optogenetic approaches that use light to activate TrkB receptors.
Source:
The optogenetic strategies presented are promising tools to investigate BDNF/TrkB signaling with tight spatial and temporal control.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Techniques
No technique tags yet.
Target processes
signalingInput: Light
Implementation Constraints
The available evidence supports that the implementation uses the aureochrome 1 light-oxygen-voltage domain from Vaucheria frigida in an optical TrkB activation design. However, the supplied material does not specify construct architecture, fusion orientation, expression system, illumination parameters, or cofactor requirements.
The supplied evidence does not report quantitative performance, activation kinetics, wavelength dependence, or downstream signaling measurements for the aureochrome 1 LOV-based construct. The same study states that a CRY2-integrated strategy was the most efficient among compared approaches, which implies this LOV-based implementation was not the top-performing design in that comparison.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Among the compared strategies, the CRY2-integrated approach that combines light-induced membrane recruitment and iTrkB homo-interaction was the most efficient at activating TrkB receptors.
By comparing all the different strategies, we find that the CRY2-integrated approach to achieve light-induced cell membrane recruitment and homo-interaction of iTrkB is most efficient in activating TrkB receptors.
Among the compared strategies, the CRY2-integrated approach that combines light-induced membrane recruitment and iTrkB homo-interaction was the most efficient at activating TrkB receptors.
By comparing all the different strategies, we find that the CRY2-integrated approach to achieve light-induced cell membrane recruitment and homo-interaction of iTrkB is most efficient in activating TrkB receptors.
Among the compared strategies, the CRY2-integrated approach that combines light-induced membrane recruitment and iTrkB homo-interaction was the most efficient at activating TrkB receptors.
By comparing all the different strategies, we find that the CRY2-integrated approach to achieve light-induced cell membrane recruitment and homo-interaction of iTrkB is most efficient in activating TrkB receptors.
Among the compared strategies, the CRY2-integrated approach that combines light-induced membrane recruitment and iTrkB homo-interaction was the most efficient at activating TrkB receptors.
By comparing all the different strategies, we find that the CRY2-integrated approach to achieve light-induced cell membrane recruitment and homo-interaction of iTrkB is most efficient in activating TrkB receptors.
Among the compared strategies, the CRY2-integrated approach that combines light-induced membrane recruitment and iTrkB homo-interaction was the most efficient at activating TrkB receptors.
By comparing all the different strategies, we find that the CRY2-integrated approach to achieve light-induced cell membrane recruitment and homo-interaction of iTrkB is most efficient in activating TrkB receptors.
Among the compared strategies, the CRY2-integrated approach that combines light-induced membrane recruitment and iTrkB homo-interaction was the most efficient at activating TrkB receptors.
By comparing all the different strategies, we find that the CRY2-integrated approach to achieve light-induced cell membrane recruitment and homo-interaction of iTrkB is most efficient in activating TrkB receptors.
Among the compared strategies, the CRY2-integrated approach that combines light-induced membrane recruitment and iTrkB homo-interaction was the most efficient at activating TrkB receptors.
By comparing all the different strategies, we find that the CRY2-integrated approach to achieve light-induced cell membrane recruitment and homo-interaction of iTrkB is most efficient in activating TrkB receptors.
The optical TrkB activation strategy is generalizable to other optical homo-dimerizers, including an aureochrome 1 LOV-domain-based implementation.
we prove that such strategies are generalizable to other optical homo-dimerizers by demonstrating the optical TrkB activation based on the light-oxygen-voltage domain of aureochrome 1 from Vaucheria frigida
The optical TrkB activation strategy is generalizable to other optical homo-dimerizers, including an aureochrome 1 LOV-domain-based implementation.
we prove that such strategies are generalizable to other optical homo-dimerizers by demonstrating the optical TrkB activation based on the light-oxygen-voltage domain of aureochrome 1 from Vaucheria frigida
The optical TrkB activation strategy is generalizable to other optical homo-dimerizers, including an aureochrome 1 LOV-domain-based implementation.
we prove that such strategies are generalizable to other optical homo-dimerizers by demonstrating the optical TrkB activation based on the light-oxygen-voltage domain of aureochrome 1 from Vaucheria frigida
The optical TrkB activation strategy is generalizable to other optical homo-dimerizers, including an aureochrome 1 LOV-domain-based implementation.
we prove that such strategies are generalizable to other optical homo-dimerizers by demonstrating the optical TrkB activation based on the light-oxygen-voltage domain of aureochrome 1 from Vaucheria frigida
The optical TrkB activation strategy is generalizable to other optical homo-dimerizers, including an aureochrome 1 LOV-domain-based implementation.
we prove that such strategies are generalizable to other optical homo-dimerizers by demonstrating the optical TrkB activation based on the light-oxygen-voltage domain of aureochrome 1 from Vaucheria frigida
The optical TrkB activation strategy is generalizable to other optical homo-dimerizers, including an aureochrome 1 LOV-domain-based implementation.
we prove that such strategies are generalizable to other optical homo-dimerizers by demonstrating the optical TrkB activation based on the light-oxygen-voltage domain of aureochrome 1 from Vaucheria frigida
The optical TrkB activation strategy is generalizable to other optical homo-dimerizers, including an aureochrome 1 LOV-domain-based implementation.
we prove that such strategies are generalizable to other optical homo-dimerizers by demonstrating the optical TrkB activation based on the light-oxygen-voltage domain of aureochrome 1 from Vaucheria frigida
A CRY2-based iTrkB optical strategy induces neurite outgrowth in PC12 cells.
the light-inducible homo-interaction of the intracellular domain of TrkB (iTrkB) in the cytosol or on the plasma membrane is able to induce the activation of downstream MAPK/ERK and PI3K/Akt signaling as well as the neurite outgrowth of PC12 cells
A CRY2-based iTrkB optical strategy induces neurite outgrowth in PC12 cells.
the light-inducible homo-interaction of the intracellular domain of TrkB (iTrkB) in the cytosol or on the plasma membrane is able to induce the activation of downstream MAPK/ERK and PI3K/Akt signaling as well as the neurite outgrowth of PC12 cells
A CRY2-based iTrkB optical strategy induces neurite outgrowth in PC12 cells.
the light-inducible homo-interaction of the intracellular domain of TrkB (iTrkB) in the cytosol or on the plasma membrane is able to induce the activation of downstream MAPK/ERK and PI3K/Akt signaling as well as the neurite outgrowth of PC12 cells
A CRY2-based iTrkB optical strategy induces neurite outgrowth in PC12 cells.
the light-inducible homo-interaction of the intracellular domain of TrkB (iTrkB) in the cytosol or on the plasma membrane is able to induce the activation of downstream MAPK/ERK and PI3K/Akt signaling as well as the neurite outgrowth of PC12 cells
A CRY2-based iTrkB optical strategy induces neurite outgrowth in PC12 cells.
the light-inducible homo-interaction of the intracellular domain of TrkB (iTrkB) in the cytosol or on the plasma membrane is able to induce the activation of downstream MAPK/ERK and PI3K/Akt signaling as well as the neurite outgrowth of PC12 cells
A CRY2-based iTrkB optical strategy induces neurite outgrowth in PC12 cells.
the light-inducible homo-interaction of the intracellular domain of TrkB (iTrkB) in the cytosol or on the plasma membrane is able to induce the activation of downstream MAPK/ERK and PI3K/Akt signaling as well as the neurite outgrowth of PC12 cells
A CRY2-based iTrkB optical strategy induces neurite outgrowth in PC12 cells.
the light-inducible homo-interaction of the intracellular domain of TrkB (iTrkB) in the cytosol or on the plasma membrane is able to induce the activation of downstream MAPK/ERK and PI3K/Akt signaling as well as the neurite outgrowth of PC12 cells
A CRY2-based iTrkB optical strategy can activate downstream MAPK/ERK and PI3K/Akt signaling.
Utilizing the photosensitive protein Arabidopsis thaliana cryptochrome 2 (CRY2), the light-inducible homo-interaction of the intracellular domain of TrkB (iTrkB) in the cytosol or on the plasma membrane is able to induce the activation of downstream MAPK/ERK and PI3K/Akt signaling
A CRY2-based iTrkB optical strategy can activate downstream MAPK/ERK and PI3K/Akt signaling.
Utilizing the photosensitive protein Arabidopsis thaliana cryptochrome 2 (CRY2), the light-inducible homo-interaction of the intracellular domain of TrkB (iTrkB) in the cytosol or on the plasma membrane is able to induce the activation of downstream MAPK/ERK and PI3K/Akt signaling
A CRY2-based iTrkB optical strategy can activate downstream MAPK/ERK and PI3K/Akt signaling.
Utilizing the photosensitive protein Arabidopsis thaliana cryptochrome 2 (CRY2), the light-inducible homo-interaction of the intracellular domain of TrkB (iTrkB) in the cytosol or on the plasma membrane is able to induce the activation of downstream MAPK/ERK and PI3K/Akt signaling
A CRY2-based iTrkB optical strategy can activate downstream MAPK/ERK and PI3K/Akt signaling.
Utilizing the photosensitive protein Arabidopsis thaliana cryptochrome 2 (CRY2), the light-inducible homo-interaction of the intracellular domain of TrkB (iTrkB) in the cytosol or on the plasma membrane is able to induce the activation of downstream MAPK/ERK and PI3K/Akt signaling
A CRY2-based iTrkB optical strategy can activate downstream MAPK/ERK and PI3K/Akt signaling.
Utilizing the photosensitive protein Arabidopsis thaliana cryptochrome 2 (CRY2), the light-inducible homo-interaction of the intracellular domain of TrkB (iTrkB) in the cytosol or on the plasma membrane is able to induce the activation of downstream MAPK/ERK and PI3K/Akt signaling
A CRY2-based iTrkB optical strategy can activate downstream MAPK/ERK and PI3K/Akt signaling.
Utilizing the photosensitive protein Arabidopsis thaliana cryptochrome 2 (CRY2), the light-inducible homo-interaction of the intracellular domain of TrkB (iTrkB) in the cytosol or on the plasma membrane is able to induce the activation of downstream MAPK/ERK and PI3K/Akt signaling
A CRY2-based iTrkB optical strategy can activate downstream MAPK/ERK and PI3K/Akt signaling.
Utilizing the photosensitive protein Arabidopsis thaliana cryptochrome 2 (CRY2), the light-inducible homo-interaction of the intracellular domain of TrkB (iTrkB) in the cytosol or on the plasma membrane is able to induce the activation of downstream MAPK/ERK and PI3K/Akt signaling
The authors developed different optogenetic approaches that use light to activate TrkB receptors.
Here we develop different optogenetic approaches that use light to activate TrkB receptors.
The authors developed different optogenetic approaches that use light to activate TrkB receptors.
Here we develop different optogenetic approaches that use light to activate TrkB receptors.
The authors developed different optogenetic approaches that use light to activate TrkB receptors.
Here we develop different optogenetic approaches that use light to activate TrkB receptors.
The authors developed different optogenetic approaches that use light to activate TrkB receptors.
Here we develop different optogenetic approaches that use light to activate TrkB receptors.
The authors developed different optogenetic approaches that use light to activate TrkB receptors.
Here we develop different optogenetic approaches that use light to activate TrkB receptors.
The authors developed different optogenetic approaches that use light to activate TrkB receptors.
Here we develop different optogenetic approaches that use light to activate TrkB receptors.
The authors developed different optogenetic approaches that use light to activate TrkB receptors.
Here we develop different optogenetic approaches that use light to activate TrkB receptors.
The presented optogenetic strategies are promising tools for investigating BDNF/TrkB signaling with tight spatial and temporal control.
The optogenetic strategies presented are promising tools to investigate BDNF/TrkB signaling with tight spatial and temporal control.
The presented optogenetic strategies are promising tools for investigating BDNF/TrkB signaling with tight spatial and temporal control.
The optogenetic strategies presented are promising tools to investigate BDNF/TrkB signaling with tight spatial and temporal control.
The presented optogenetic strategies are promising tools for investigating BDNF/TrkB signaling with tight spatial and temporal control.
The optogenetic strategies presented are promising tools to investigate BDNF/TrkB signaling with tight spatial and temporal control.
The presented optogenetic strategies are promising tools for investigating BDNF/TrkB signaling with tight spatial and temporal control.
The optogenetic strategies presented are promising tools to investigate BDNF/TrkB signaling with tight spatial and temporal control.
The presented optogenetic strategies are promising tools for investigating BDNF/TrkB signaling with tight spatial and temporal control.
The optogenetic strategies presented are promising tools to investigate BDNF/TrkB signaling with tight spatial and temporal control.
The presented optogenetic strategies are promising tools for investigating BDNF/TrkB signaling with tight spatial and temporal control.
The optogenetic strategies presented are promising tools to investigate BDNF/TrkB signaling with tight spatial and temporal control.
The presented optogenetic strategies are promising tools for investigating BDNF/TrkB signaling with tight spatial and temporal control.
The optogenetic strategies presented are promising tools to investigate BDNF/TrkB signaling with tight spatial and temporal control.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug aureochrome-1-lov-domain-based-optical-trkb-activation-approach
we prove that such strategies are generalizable to other optical homo-dimerizers by demonstrating the optical TrkB activation based on the light-oxygen-voltage domain of aureochrome 1 from Vaucheria frigida
Source:
generalizabilitysupports
The optical TrkB activation strategy is generalizable to other optical homo-dimerizers, including an aureochrome 1 LOV-domain-based implementation.
we prove that such strategies are generalizable to other optical homo-dimerizers by demonstrating the optical TrkB activation based on the light-oxygen-voltage domain of aureochrome 1 from Vaucheria frigida
Source:
tool developmentsupports
The authors developed different optogenetic approaches that use light to activate TrkB receptors.
Here we develop different optogenetic approaches that use light to activate TrkB receptors.
Source:
use casesupports
The presented optogenetic strategies are promising tools for investigating BDNF/TrkB signaling with tight spatial and temporal control.
The optogenetic strategies presented are promising tools to investigate BDNF/TrkB signaling with tight spatial and temporal control.
Source:
Comparisons
Source-backed strengths
Its main demonstrated strength is conceptual generalizability: the study explicitly states that optical TrkB activation was demonstrated using the aureochrome 1 LOV domain from Vaucheria frigida. This supports the idea that TrkB optogenetic activation can be achieved with multiple optical homo-dimerizers.
Source:
By comparing all the different strategies, we find that the CRY2-integrated approach to achieve light-induced cell membrane recruitment and homo-interaction of iTrkB is most efficient in activating TrkB receptors.
Ranked Citations
- 1.