Source 1review2024Journal of Biological Chemistry
Toolkit/BACCS
BACCS
Also known as: BACCS, blue light-activated Ca(2+) channel switch
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
BACCS is a genetically engineered blue light-activated Ca2+ channel switch developed as an optogenetic tool for generating intracellular Ca2+ signals. It acts by opening Ca2+-selective ORAI ion channels in response to blue light and has been used to drive downstream cellular and physiological responses.
Usefulness & Problems
Why this is useful
BACCS provides optical control over intracellular Ca2+ signaling, enabling noninvasive induction of Ca2+-dependent processes with light. Reported applications include control of NFAT-mediated gene expression and elicitation of light-dependent electrophysiological responses in the mouse olfactory system.
Source:
In the mouse olfactory system, BACCS mediates light-dependent electrophysiological responses.
Source:
Using BACCSs, we successfully control cellular events including NFAT-mediated gene expression.
Source:
Thus, BACCSs are a useful optogenetic tool for generating temporally various intracellular Ca(2+) signals with a large dynamic range, and will be applicable to both in vitro and in vivo studies.
Problem solved
BACCS addresses the need for a genetically encoded method to generate intracellular Ca2+ signals with light. It specifically enables blue light-triggered activation of ORAI-mediated Ca2+ entry rather than relying on endogenous stimulation pathways.
Source:
In the mouse olfactory system, BACCS mediates light-dependent electrophysiological responses.
Source:
Using BACCSs, we successfully control cellular events including NFAT-mediated gene expression.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
Computational DesignTarget processes
signalingInput: Light
Implementation Constraints
The available evidence supports that BACCS is a genetically encoded, multi-component blue light-responsive system that functions through ORAI Ca2+ channels. Mutagenesis was used to generate variants with different intracellular Ca2+ recovery kinetics. The provided material does not specify construct architecture, chromophore requirements, expression strategy, or delivery method.
The supplied evidence does not provide quantitative performance metrics such as activation wavelength range, conductance, dynamic range, Ca2+ selectivity values, or temporal precision. The evidence base here is limited to a single 2015 source citation, so independent replication is not established from the provided material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2review2021Cells
Source 3primary paper2015Nature Communications
Source 4review2017Cell Calcium
Ranked Claims
The review scaffold groups STIM1 optogenetic tools into at least CRY2-based oligomerization designs and LOV2-based unfolding or caging designs for optical control of calcium signaling.
The review scaffold explicitly names OptoSTIM1, monSTIM1, eOS1, Opto-CRAC1, Opto-CRAC2, BACCS, and LOVS1K as STIM1-related optogenetic calcium-control tools or variants within the review scope.
The review covers optogenetic tools for precise control of Ca2+-permeable ion channels, receptors, and associated downstream signaling cascades.
Here, we review the various optogenetic tools that have been used to achieve precise control over different Ca2+-permeable ion channels and receptors and associated downstream signaling cascades.
This review covers an optogenetic toolkit for precise control of calcium signaling, including genetically encoded calcium actuators and multiple mechanistic classes such as STIM1/CRAC-based, GPCR-based, RTK-based, and channel-based approaches.
Melanopsin and Opto-XRs are discussed in the review as GPCR-based optogenetic routes relevant to calcium signaling control.
Opto-RTKs are discussed in the review as receptor-tyrosine-kinase-based optogenetic tools within the calcium-control toolkit.
OptoSTIM1 and Opto-CRAC are discussed in the review as STIM1/CRAC-based optogenetic tools for controlling calcium signaling.
PACR is discussed in the review as a genetically encoded photoactivatable calcium releaser for optical control of calcium signaling.
BACCS mediates light-dependent electrophysiological responses in the mouse olfactory system.
In the mouse olfactory system, BACCS mediates light-dependent electrophysiological responses.
BACCS mediates light-dependent electrophysiological responses in the mouse olfactory system.
In the mouse olfactory system, BACCS mediates light-dependent electrophysiological responses.
BACCS mediates light-dependent electrophysiological responses in the mouse olfactory system.
In the mouse olfactory system, BACCS mediates light-dependent electrophysiological responses.
BACCS mediates light-dependent electrophysiological responses in the mouse olfactory system.
In the mouse olfactory system, BACCS mediates light-dependent electrophysiological responses.
BACCS mediates light-dependent electrophysiological responses in the mouse olfactory system.
In the mouse olfactory system, BACCS mediates light-dependent electrophysiological responses.
BACCS mediates light-dependent electrophysiological responses in the mouse olfactory system.
In the mouse olfactory system, BACCS mediates light-dependent electrophysiological responses.
BACCS mediates light-dependent electrophysiological responses in the mouse olfactory system.
In the mouse olfactory system, BACCS mediates light-dependent electrophysiological responses.
BACCSs can control cellular events including NFAT-mediated gene expression.
Using BACCSs, we successfully control cellular events including NFAT-mediated gene expression.
BACCSs can control cellular events including NFAT-mediated gene expression.
Using BACCSs, we successfully control cellular events including NFAT-mediated gene expression.
BACCSs can control cellular events including NFAT-mediated gene expression.
Using BACCSs, we successfully control cellular events including NFAT-mediated gene expression.
BACCSs can control cellular events including NFAT-mediated gene expression.
Using BACCSs, we successfully control cellular events including NFAT-mediated gene expression.
BACCSs can control cellular events including NFAT-mediated gene expression.
Using BACCSs, we successfully control cellular events including NFAT-mediated gene expression.
BACCSs can control cellular events including NFAT-mediated gene expression.
Using BACCSs, we successfully control cellular events including NFAT-mediated gene expression.
BACCSs can control cellular events including NFAT-mediated gene expression.
Using BACCSs, we successfully control cellular events including NFAT-mediated gene expression.
BACCS mutants were generated that exhibit fast and slow recovery of intracellular Ca2+.
Furthermore, we generate BACCS mutants, which exhibit fast and slow recovery of intracellular Ca(2+).
BACCS mutants were generated that exhibit fast and slow recovery of intracellular Ca2+.
Furthermore, we generate BACCS mutants, which exhibit fast and slow recovery of intracellular Ca(2+).
BACCS mutants were generated that exhibit fast and slow recovery of intracellular Ca2+.
Furthermore, we generate BACCS mutants, which exhibit fast and slow recovery of intracellular Ca(2+).
BACCS mutants were generated that exhibit fast and slow recovery of intracellular Ca2+.
Furthermore, we generate BACCS mutants, which exhibit fast and slow recovery of intracellular Ca(2+).
BACCS mutants were generated that exhibit fast and slow recovery of intracellular Ca2+.
Furthermore, we generate BACCS mutants, which exhibit fast and slow recovery of intracellular Ca(2+).
BACCS mutants were generated that exhibit fast and slow recovery of intracellular Ca2+.
Furthermore, we generate BACCS mutants, which exhibit fast and slow recovery of intracellular Ca(2+).
BACCS mutants were generated that exhibit fast and slow recovery of intracellular Ca2+.
Furthermore, we generate BACCS mutants, which exhibit fast and slow recovery of intracellular Ca(2+).
BACCS opens Ca2+-selective ORAI ion channels in response to light.
BACCS opens Ca(2+)-selective ORAI ion channels in response to light.
BACCS opens Ca2+-selective ORAI ion channels in response to light.
BACCS opens Ca(2+)-selective ORAI ion channels in response to light.
BACCS opens Ca2+-selective ORAI ion channels in response to light.
BACCS opens Ca(2+)-selective ORAI ion channels in response to light.
BACCS opens Ca2+-selective ORAI ion channels in response to light.
BACCS opens Ca(2+)-selective ORAI ion channels in response to light.
BACCS opens Ca2+-selective ORAI ion channels in response to light.
BACCS opens Ca(2+)-selective ORAI ion channels in response to light.
BACCS opens Ca2+-selective ORAI ion channels in response to light.
BACCS opens Ca(2+)-selective ORAI ion channels in response to light.
BACCS opens Ca2+-selective ORAI ion channels in response to light.
BACCS opens Ca(2+)-selective ORAI ion channels in response to light.
dmBACCS2 combined with Drosophila Orai elevates Ca2+ more rapidly, with Ca2+ elevation in mammalian cells observed within 1 s of light exposure.
A BACCS variant, dmBACCS2, combined with Drosophila Orai, elevates the Ca(2+) concentration more rapidly, such that Ca(2+) elevation in mammalian cells is observed within 1 s on light exposure.
time to Ca2+ elevation 1 s
dmBACCS2 combined with Drosophila Orai elevates Ca2+ more rapidly, with Ca2+ elevation in mammalian cells observed within 1 s of light exposure.
A BACCS variant, dmBACCS2, combined with Drosophila Orai, elevates the Ca(2+) concentration more rapidly, such that Ca(2+) elevation in mammalian cells is observed within 1 s on light exposure.
time to Ca2+ elevation 1 s
dmBACCS2 combined with Drosophila Orai elevates Ca2+ more rapidly, with Ca2+ elevation in mammalian cells observed within 1 s of light exposure.
A BACCS variant, dmBACCS2, combined with Drosophila Orai, elevates the Ca(2+) concentration more rapidly, such that Ca(2+) elevation in mammalian cells is observed within 1 s on light exposure.
time to Ca2+ elevation 1 s
dmBACCS2 combined with Drosophila Orai elevates Ca2+ more rapidly, with Ca2+ elevation in mammalian cells observed within 1 s of light exposure.
A BACCS variant, dmBACCS2, combined with Drosophila Orai, elevates the Ca(2+) concentration more rapidly, such that Ca(2+) elevation in mammalian cells is observed within 1 s on light exposure.
time to Ca2+ elevation 1 s
dmBACCS2 combined with Drosophila Orai elevates Ca2+ more rapidly, with Ca2+ elevation in mammalian cells observed within 1 s of light exposure.
A BACCS variant, dmBACCS2, combined with Drosophila Orai, elevates the Ca(2+) concentration more rapidly, such that Ca(2+) elevation in mammalian cells is observed within 1 s on light exposure.
time to Ca2+ elevation 1 s
dmBACCS2 combined with Drosophila Orai elevates Ca2+ more rapidly, with Ca2+ elevation in mammalian cells observed within 1 s of light exposure.
A BACCS variant, dmBACCS2, combined with Drosophila Orai, elevates the Ca(2+) concentration more rapidly, such that Ca(2+) elevation in mammalian cells is observed within 1 s on light exposure.
time to Ca2+ elevation 1 s
dmBACCS2 combined with Drosophila Orai elevates Ca2+ more rapidly, with Ca2+ elevation in mammalian cells observed within 1 s of light exposure.
A BACCS variant, dmBACCS2, combined with Drosophila Orai, elevates the Ca(2+) concentration more rapidly, such that Ca(2+) elevation in mammalian cells is observed within 1 s on light exposure.
time to Ca2+ elevation 1 s
BACCSs are a useful optogenetic tool for generating temporally various intracellular Ca2+ signals with a large dynamic range and are applicable to both in vitro and in vivo studies.
Thus, BACCSs are a useful optogenetic tool for generating temporally various intracellular Ca(2+) signals with a large dynamic range, and will be applicable to both in vitro and in vivo studies.
BACCSs are a useful optogenetic tool for generating temporally various intracellular Ca2+ signals with a large dynamic range and are applicable to both in vitro and in vivo studies.
Thus, BACCSs are a useful optogenetic tool for generating temporally various intracellular Ca(2+) signals with a large dynamic range, and will be applicable to both in vitro and in vivo studies.
BACCSs are a useful optogenetic tool for generating temporally various intracellular Ca2+ signals with a large dynamic range and are applicable to both in vitro and in vivo studies.
Thus, BACCSs are a useful optogenetic tool for generating temporally various intracellular Ca(2+) signals with a large dynamic range, and will be applicable to both in vitro and in vivo studies.
BACCSs are a useful optogenetic tool for generating temporally various intracellular Ca2+ signals with a large dynamic range and are applicable to both in vitro and in vivo studies.
Thus, BACCSs are a useful optogenetic tool for generating temporally various intracellular Ca(2+) signals with a large dynamic range, and will be applicable to both in vitro and in vivo studies.
BACCSs are a useful optogenetic tool for generating temporally various intracellular Ca2+ signals with a large dynamic range and are applicable to both in vitro and in vivo studies.
Thus, BACCSs are a useful optogenetic tool for generating temporally various intracellular Ca(2+) signals with a large dynamic range, and will be applicable to both in vitro and in vivo studies.
BACCSs are a useful optogenetic tool for generating temporally various intracellular Ca2+ signals with a large dynamic range and are applicable to both in vitro and in vivo studies.
Thus, BACCSs are a useful optogenetic tool for generating temporally various intracellular Ca(2+) signals with a large dynamic range, and will be applicable to both in vitro and in vivo studies.
BACCSs are a useful optogenetic tool for generating temporally various intracellular Ca2+ signals with a large dynamic range and are applicable to both in vitro and in vivo studies.
Thus, BACCSs are a useful optogenetic tool for generating temporally various intracellular Ca(2+) signals with a large dynamic range, and will be applicable to both in vitro and in vivo studies.
Approval Evidence
4 sources9 linked approval claimsfirst-pass slug baccs
The supplied web research summary states that the anchor review explicitly lists BACCS as a named LOV2-based calcium actuator.
Source:
Web research summary for this review identifies BACCS as an explicitly identified STIM1/ORAI-related optogenetic Ca2+ actuator within the review topic.
Source:
The supplied web research summary identifies BACCS as a blue-light-activated Ca2+ channel switch in the calcium optogenetics space and notes that anchor PMC text references hBACCS2/dmBACCS2 in ORAI-related implementation discussion.
Source:
Here we report a genetically engineered blue light-activated Ca(2+) channel switch (BACCS), as an optogenetic tool for generating Ca(2+) signals.
Source:
design mechanism summarysupports
The review scaffold groups STIM1 optogenetic tools into at least CRY2-based oligomerization designs and LOV2-based unfolding or caging designs for optical control of calcium signaling.
Source:
tool family membershipsupports
The review scaffold explicitly names OptoSTIM1, monSTIM1, eOS1, Opto-CRAC1, Opto-CRAC2, BACCS, and LOVS1K as STIM1-related optogenetic calcium-control tools or variants within the review scope.
Source:
review scope statementsupports
The review covers optogenetic tools for precise control of Ca2+-permeable ion channels, receptors, and associated downstream signaling cascades.
Here, we review the various optogenetic tools that have been used to achieve precise control over different Ca2+-permeable ion channels and receptors and associated downstream signaling cascades.
Source:
review scopesupports
This review covers an optogenetic toolkit for precise control of calcium signaling, including genetically encoded calcium actuators and multiple mechanistic classes such as STIM1/CRAC-based, GPCR-based, RTK-based, and channel-based approaches.
Source:
applicationsupports
BACCS mediates light-dependent electrophysiological responses in the mouse olfactory system.
In the mouse olfactory system, BACCS mediates light-dependent electrophysiological responses.
Source:
applicationsupports
BACCSs can control cellular events including NFAT-mediated gene expression.
Using BACCSs, we successfully control cellular events including NFAT-mediated gene expression.
Source:
engineeringsupports
BACCS mutants were generated that exhibit fast and slow recovery of intracellular Ca2+.
Furthermore, we generate BACCS mutants, which exhibit fast and slow recovery of intracellular Ca(2+).
Source:
mechanismsupports
BACCS opens Ca2+-selective ORAI ion channels in response to light.
BACCS opens Ca(2+)-selective ORAI ion channels in response to light.
Source:
utilitysupports
BACCSs are a useful optogenetic tool for generating temporally various intracellular Ca2+ signals with a large dynamic range and are applicable to both in vitro and in vivo studies.
Thus, BACCSs are a useful optogenetic tool for generating temporally various intracellular Ca(2+) signals with a large dynamic range, and will be applicable to both in vitro and in vivo studies.
Source:
Comparisons
Source-backed strengths
The tool is explicitly described as genetically encoded and blue light-activated, supporting direct optical induction of intracellular Ca2+ signals. Source claims indicate functional output at multiple levels, including NFAT-mediated transcriptional control and electrophysiological responses in the mouse olfactory system. BACCS mutants with fast and slow recovery of intracellular Ca2+ were also generated, indicating tunable response kinetics.
Source:
Furthermore, we generate BACCS mutants, which exhibit fast and slow recovery of intracellular Ca(2+).
Source:
A BACCS variant, dmBACCS2, combined with Drosophila Orai, elevates the Ca(2+) concentration more rapidly, such that Ca(2+) elevation in mammalian cells is observed within 1 s on light exposure.
Ranked Citations
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