Source 1primary paper2020Genome biology
Toolkit/bacteriophage-derived peptides
bacteriophage-derived peptides
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Bacteriophage-derived peptides are peptide inhibitors reported to allosterically inhibit CRISPR-Cas9. Based on the supplied evidence, they act as anti-CRISPR-like modulators of Cas9 activity in genome editing contexts.
Usefulness & Problems
Why this is useful
These peptides are useful as molecular regulators of CRISPR-Cas9 because they provide a peptide-based route to suppress Cas9 activity. The supplied evidence supports their relevance to genome editing control, but does not provide detailed comparative performance data.
Problem solved
They address the problem of limiting or modulating CRISPR-Cas9 nuclease activity during editing applications. The evidence specifically supports allosteric inhibition of Cas9, but does not define the exact experimental use cases or operating window.
Problem links
Need controllable genome or transcript editing
DerivedBacteriophage-derived peptides are peptide inhibitors reported to allosterically inhibit CRISPR-Cas9. Based on the supplied evidence, they function as anti-CRISPR-like modulators of Cas9 activity in genome editing contexts.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
editingImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: actuatoroperating role: regulatorswitch architecture: uncaging
Implementation is only supported at the level of using bacteriophage-derived peptides to inhibit CRISPR-Cas9. The provided evidence does not describe construct architecture, delivery method, expression strategy, dosage, or cofactor requirements.
The supplied evidence is limited to a title-level claim and does not specify peptide sequences, Cas9 ortholog scope, potency, structural determinants, or cellular validation details. Independent replication and breadth across editing systems cannot be established from the provided material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Bacteriophage-derived peptides allosterically inhibit CRISPR-Cas9.
Bacteriophage-derived peptides allosterically inhibit CRISPR-Cas9.
Bacteriophage-derived peptides allosterically inhibit CRISPR-Cas9.
Bacteriophage-derived peptides allosterically inhibit CRISPR-Cas9.
Bacteriophage-derived peptides allosterically inhibit CRISPR-Cas9.
Bacteriophage-derived peptides allosterically inhibit CRISPR-Cas9.
Bacteriophage-derived peptides allosterically inhibit CRISPR-Cas9.
Bacteriophage-derived peptides allosterically inhibit CRISPR-Cas9.
Bacteriophage-derived peptides allosterically inhibit CRISPR-Cas9.
Bacteriophage-derived peptides allosterically inhibit CRISPR-Cas9.
Bacteriophage-derived peptides allosterically inhibit CRISPR-Cas9.
Bacteriophage-derived peptides allosterically inhibit CRISPR-Cas9.
Bacteriophage-derived peptides allosterically inhibit CRISPR-Cas9.
Bacteriophage-derived peptides allosterically inhibit CRISPR-Cas9.
Bacteriophage-derived peptides allosterically inhibit CRISPR-Cas9.
Bacteriophage-derived peptides allosterically inhibit CRISPR-Cas9.
Bacteriophage-derived peptides allosterically inhibit CRISPR-Cas9.
Approval Evidence
1 source1 linked approval claimfirst-pass slug bacteriophage-derived-peptides
Allosteric inhibition of CRISPR-Cas9 by bacteriophage-derived peptides
Source:
inhibitionsupports
Bacteriophage-derived peptides allosterically inhibit CRISPR-Cas9.
Source:
Comparisons
Source-backed strengths
A key strength is that inhibition is reported to occur through an allosteric mechanism rather than by simple active-site blockade. The evidence also indicates that the inhibitors are bacteriophage-derived peptides, supporting a compact peptide-based modality for Cas9 regulation.
bacteriophage-derived peptides and CRISPR-Cas genome editors directly modulated by temperature address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing
Compared with microhomology templates
bacteriophage-derived peptides and microhomology templates address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing
Compared with PMNT mixed with single-stranded DNA color reporter
bacteriophage-derived peptides and PMNT mixed with single-stranded DNA color reporter address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing
Ranked Citations
- 1.