Source 1primary paper2014Nucleic Acids Research
Toolkit/benzoate-/vanillate-responsive mammalian gene switch
benzoate-/vanillate-responsive mammalian gene switch
Also known as: benzoate-/vanillate-responsive device, dual-input mammalian gene switch
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The benzoate-/vanillate-responsive mammalian gene switch is a dual-input transgene control system engineered for mammalian cells that is induced and repressed by the food additives benzoate and vanillate. It was also reported to function as a modular component in higher-order gene control networks and to regulate a SEAP reporter in implanted designer cells in mice.
Usefulness & Problems
Why this is useful
This system provides small-molecule control of mammalian transgene expression using benzoate and vanillate as antagonistic inputs. It is useful as a modular regulatory element because it was reported to be compatible with other transgene control systems and incorporable into higher-order control networks.
Source:
we have designed different mammalian gene expression systems that could be induced and repressed by the food additives benzoate and vanillate
Source:
When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
Problem solved
It addresses the need for a mammalian gene switch that can integrate two chemical inputs to induce or repress gene expression. The reported design also helps solve the problem of building combinatorial and higher-order mammalian gene control circuits from compatible regulatory modules.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
Computational DesignTarget processes
recombinationImplementation Constraints
The available evidence indicates use in mammalian gene expression systems and validation with a SEAP reporter in implanted designer cells in mice. However, the supplied material does not describe construct design, delivery method, host cell type, or any required cofactors or expression constraints.
The supplied evidence does not specify the molecular components, promoter architecture, dynamic range, response kinetics, or dose-response characteristics of the switch. Independent replication is not provided in the supplied record, and in vivo evidence is limited to SEAP regulation in implanted designer cells in mice.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successMouseapplication demo
Inferred from claim c2 during normalization. In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program. Derived from claim c2. Quoted text: When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
Source:
successMouseapplication demo
Inferred from claim c2 during normalization. In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program. Derived from claim c2. Quoted text: When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
Source:
successMouseapplication demo
Inferred from claim c2 during normalization. In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program. Derived from claim c2. Quoted text: When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
Source:
successMouseapplication demo
Inferred from claim c2 during normalization. In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program. Derived from claim c2. Quoted text: When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
Source:
successMouseapplication demo
Inferred from claim c2 during normalization. In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program. Derived from claim c2. Quoted text: When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
Source:
successMouseapplication demo
Inferred from claim c2 during normalization. In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program. Derived from claim c2. Quoted text: When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
Source:
successMouseapplication demo
Inferred from claim c2 during normalization. In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program. Derived from claim c2. Quoted text: When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
Source:
Supporting Sources
Ranked Claims
The benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks.
the benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks
The benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks.
the benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks
The benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks.
the benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks
The benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks.
the benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks
The benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks.
the benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks
The benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks.
the benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks
The benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks.
the benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks
The authors designed mammalian gene expression systems that can be induced and repressed by the food additives benzoate and vanillate.
we have designed different mammalian gene expression systems that could be induced and repressed by the food additives benzoate and vanillate
The authors designed mammalian gene expression systems that can be induced and repressed by the food additives benzoate and vanillate.
we have designed different mammalian gene expression systems that could be induced and repressed by the food additives benzoate and vanillate
The authors designed mammalian gene expression systems that can be induced and repressed by the food additives benzoate and vanillate.
we have designed different mammalian gene expression systems that could be induced and repressed by the food additives benzoate and vanillate
The authors designed mammalian gene expression systems that can be induced and repressed by the food additives benzoate and vanillate.
we have designed different mammalian gene expression systems that could be induced and repressed by the food additives benzoate and vanillate
The authors designed mammalian gene expression systems that can be induced and repressed by the food additives benzoate and vanillate.
we have designed different mammalian gene expression systems that could be induced and repressed by the food additives benzoate and vanillate
The authors designed mammalian gene expression systems that can be induced and repressed by the food additives benzoate and vanillate.
we have designed different mammalian gene expression systems that could be induced and repressed by the food additives benzoate and vanillate
The authors designed mammalian gene expression systems that can be induced and repressed by the food additives benzoate and vanillate.
we have designed different mammalian gene expression systems that could be induced and repressed by the food additives benzoate and vanillate
In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program.
When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program.
When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program.
When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program.
When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program.
When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program.
When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program.
When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
Higher-order control networks assembled from the benzoate-/vanillate-responsive device provided expression dynamics reminiscent of a lap-timing stopwatch.
could be assembled into higher-order control networks providing expression dynamics reminiscent of a lap-timing stopwatch
Higher-order control networks assembled from the benzoate-/vanillate-responsive device provided expression dynamics reminiscent of a lap-timing stopwatch.
could be assembled into higher-order control networks providing expression dynamics reminiscent of a lap-timing stopwatch
Higher-order control networks assembled from the benzoate-/vanillate-responsive device provided expression dynamics reminiscent of a lap-timing stopwatch.
could be assembled into higher-order control networks providing expression dynamics reminiscent of a lap-timing stopwatch
Higher-order control networks assembled from the benzoate-/vanillate-responsive device provided expression dynamics reminiscent of a lap-timing stopwatch.
could be assembled into higher-order control networks providing expression dynamics reminiscent of a lap-timing stopwatch
Higher-order control networks assembled from the benzoate-/vanillate-responsive device provided expression dynamics reminiscent of a lap-timing stopwatch.
could be assembled into higher-order control networks providing expression dynamics reminiscent of a lap-timing stopwatch
Higher-order control networks assembled from the benzoate-/vanillate-responsive device provided expression dynamics reminiscent of a lap-timing stopwatch.
could be assembled into higher-order control networks providing expression dynamics reminiscent of a lap-timing stopwatch
Higher-order control networks assembled from the benzoate-/vanillate-responsive device provided expression dynamics reminiscent of a lap-timing stopwatch.
could be assembled into higher-order control networks providing expression dynamics reminiscent of a lap-timing stopwatch
Approval Evidence
1 source4 linked approval claimsfirst-pass slug benzoate-vanillate-responsive-mammalian-gene-switch
we have designed different mammalian gene expression systems that could be induced and repressed by the food additives benzoate and vanillate
Source:
compatibilitysupports
The benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks.
the benzoate-/vanillate-responsive device was compatible with other transgene control systems and could be assembled into higher-order control networks
Source:
functional responsesupports
The authors designed mammalian gene expression systems that can be induced and repressed by the food additives benzoate and vanillate.
we have designed different mammalian gene expression systems that could be induced and repressed by the food additives benzoate and vanillate
Source:
in vivo responsesupports
In mice implanted with designer cells expressing SEAP under gene-switch control, blood SEAP levels directly correlated with a benzoate-enriched drinking program.
When implanting designer cells engineered for gene switch-driven expression of the human placental secreted alkaline phosphatase (SEAP) into mice, blood SEAP levels of treated animals directly correlated with a benzoate-enriched drinking programme.
Source:
network dynamicssupports
Higher-order control networks assembled from the benzoate-/vanillate-responsive device provided expression dynamics reminiscent of a lap-timing stopwatch.
could be assembled into higher-order control networks providing expression dynamics reminiscent of a lap-timing stopwatch
Source:
Comparisons
Source-backed strengths
The device was reported to support both induction and repression of mammalian gene expression in response to benzoate and vanillate. It showed compatibility with other transgene control systems and was assembled into higher-order control networks. In vivo relevance was supported by implanted designer cells in mice, where blood SEAP levels directly correlated with a benzoate-enriched drinking program.
Ranked Citations
- 1.