Source 1primary paper2025MED
Toolkit/BLU-VIPR
BLU-VIPR
Also known as: Blue Light-inducible Universal VPR-Improved Production of RGRs
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Here, we present a new optogenetic CRISPR tool (Blue Light-inducible Universal VPR-Improved Production of RGRs, BLU-VIPR)...
Usefulness & Problems
No literature-backed usefulness or problem-fit explainer has been materialized for this record yet.
Published Workflows
Objective: Engineer an optogenetic CRISPR system that enables precise spatiotemporal gene perturbation by light-controlled guide RNA production, including in vivo use in T lymphocytes.
Why it works: The paper frames the approach as combining CRISPR flexibility with light's spatiotemporal resolution, while using a genetically encoded design that precisely excises multiple gRNAs from one transcript.
blue-light activated transcriptionoptogenetic regulation of gRNA productionribozyme-mediated excision of multiple gRNAs from a single transcriptengineering around a light-activated transcription factorengineering ribozyme-flanked gRNA expression architecture
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
light-inducible transcriptional activationoptogenetic regulation of grna productionribozyme-mediated grna excisionTechniques
Computational DesignTarget processes
editingtranscriptionInput: Light
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
BLU-VIPR enabled several types of optogenetic CRISPR including indels, CRISPRa, and base editing.
This simplified spatiotemporal gene perturbation and allowed for several types of optogenetic CRISPR, including indels, CRISPRa, and base editing.
BLU-VIPR was engineered around VPR-EL222 and ribozyme-flanked gRNA.
We engineered BLU-VIPR around a new potent blue-light activated transcription factor (VPR-EL222) and ribozyme-flanked gRNA.
The BLU-VIPR design ensures precise excision of multiple gRNAs from a single messenger RNA transcript.
The BLU-VIPR design is genetically encoded and ensures precise excision of multiple gRNAs from a single messenger RNA transcript.
BLU-VIPR worked in vivo and achieved optogenetic gene editing in T lymphocytes in vivo.
BLU-VIPR also worked in vivo with cells previously intractable to optogenetic gene editing, achieving optogenetic gene editing in T lymphocytes in vivo.
BLU-VIPR diverges from split-Cas strategies by optogenetically regulating gRNA production.
BLU-VIPR) that diverges from prevailing split-Cas design strategies and instead focuses on optogenetic regulation of guide RNA (gRNA) production.
BLU-VIPR is a new optogenetic CRISPR tool for light-controlled gene perturbation.
Here, we present a new optogenetic CRISPR tool (Blue Light-inducible Universal VPR-Improved Production of RGRs, BLU-VIPR)...
Approval Evidence
1 source6 linked approval claimsfirst-pass slug blu-vipr
Here, we present a new optogenetic CRISPR tool (Blue Light-inducible Universal VPR-Improved Production of RGRs, BLU-VIPR)...
Source:
application scopesupports
BLU-VIPR enabled several types of optogenetic CRISPR including indels, CRISPRa, and base editing.
This simplified spatiotemporal gene perturbation and allowed for several types of optogenetic CRISPR, including indels, CRISPRa, and base editing.
Source:
design featuresupports
BLU-VIPR was engineered around VPR-EL222 and ribozyme-flanked gRNA.
We engineered BLU-VIPR around a new potent blue-light activated transcription factor (VPR-EL222) and ribozyme-flanked gRNA.
Source:
functional capabilitysupports
The BLU-VIPR design ensures precise excision of multiple gRNAs from a single messenger RNA transcript.
The BLU-VIPR design is genetically encoded and ensures precise excision of multiple gRNAs from a single messenger RNA transcript.
Source:
in vivo applicationsupports
BLU-VIPR worked in vivo and achieved optogenetic gene editing in T lymphocytes in vivo.
BLU-VIPR also worked in vivo with cells previously intractable to optogenetic gene editing, achieving optogenetic gene editing in T lymphocytes in vivo.
Source:
mechanismsupports
BLU-VIPR diverges from split-Cas strategies by optogenetically regulating gRNA production.
BLU-VIPR) that diverges from prevailing split-Cas design strategies and instead focuses on optogenetic regulation of guide RNA (gRNA) production.
Source:
tool introductionsupports
BLU-VIPR is a new optogenetic CRISPR tool for light-controlled gene perturbation.
Here, we present a new optogenetic CRISPR tool (Blue Light-inducible Universal VPR-Improved Production of RGRs, BLU-VIPR)...
Source:
Comparisons
No literature-backed comparison notes have been materialized for this record yet.
Ranked Citations
- 1.