Source 1primary paper2023Journal of the American Chemical Society
Toolkit/blue-light-activated DNA template ON switch
blue-light-activated DNA template ON switch
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The blue-light-activated DNA template ON switch is a light-responsive DNA construct that enables transcription to be initiated by blue light. In the reported combined system, it was used with a separate light-controlled OFF switch to start transcription with one wavelength and later halt translation of the corresponding mRNA-to-protein output with a different wavelength.
Usefulness & Problems
Why this is useful
This tool is useful for remote, wavelength-specific control of gene expression timing in cell-free systems. The cited study shows that it can provide orthogonal optical initiation of transcription when combined with a second light-responsive construct that stops downstream translation.
Problem solved
It helps solve the problem of externally controlling when transcription begins using light, while remaining compatible with a second optical control layer acting at translation. The reported application specifically addresses multi-timepoint, multiwavelength regulation of gene expression in a combined transcription-translation workflow.
Problem links
Need precise spatiotemporal control with light input
DerivedThe blue-light-activated DNA template ON switch is a light-responsive DNA construct that enables transcription to be initiated by blue light. In the reported combined system, it was used with a separate light-controlled OFF switch to start transcription with one wavelength and later halt translation of the corresponding mRNA-to-protein output with a different wavelength.
Need tighter control over gene expression timing or amplitude
DerivedThe blue-light-activated DNA template ON switch is a light-responsive DNA construct that enables transcription to be initiated by blue light. In the reported combined system, it was used with a separate light-controlled OFF switch to start transcription with one wavelength and later halt translation of the corresponding mRNA-to-protein output with a different wavelength.
Need tighter control over protein production
DerivedThe blue-light-activated DNA template ON switch is a light-responsive DNA construct that enables transcription to be initiated by blue light. In the reported combined system, it was used with a separate light-controlled OFF switch to start transcription with one wavelength and later halt translation of the corresponding mRNA-to-protein output with a different wavelength.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
light-activated transcriptional switchingorthogonal wavelength-dependent controlTranslation ControlTechniques
No technique tags yet.
Target processes
transcriptiontranslationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulator
The available evidence indicates that this is a DNA template-based construct used in conjunction with a separate light-controlled OFF switch in a cell-free expression context. The source summary associates the broader system with photocaged nucleic acids, but the provided excerpt does not specify construct architecture, photocage chemistry, or illumination parameters for this ON switch alone.
The provided evidence only documents performance in a combined system and does not supply standalone quantitative characterization of the blue-light-activated DNA template ON switch. No details are provided here on activation wavelength, dynamic range, sequence design, kinetics, or validation beyond the cited report.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successCell-freeapplication demo
Inferred from claim c4 during normalization. Combining the blue-light-activated DNA template ON switch with the light-controlled OFF switch allowed transcription to be started with one wavelength and translation of the corresponding mRNA to protein to be halted with a different wavelength at multiple timepoints. Derived from claim c4. Section: abstract. Quoted text: By combining with our previously generated blue-light-activated DNA template ON switch, we were able to start transcription with one wavelength of light and then halt the translation of the corresponding mRNA to protein with a different wavelength, at multiple timepoints.
Source:
successCell-freeapplication demo
Inferred from claim c4 during normalization. Combining the blue-light-activated DNA template ON switch with the light-controlled OFF switch allowed transcription to be started with one wavelength and translation of the corresponding mRNA to protein to be halted with a different wavelength at multiple timepoints. Derived from claim c4. Section: abstract. Quoted text: By combining with our previously generated blue-light-activated DNA template ON switch, we were able to start transcription with one wavelength of light and then halt the translation of the corresponding mRNA to protein with a different wavelength, at multiple timepoints.
Source:
successCell-freeapplication demo
Inferred from claim c4 during normalization. Combining the blue-light-activated DNA template ON switch with the light-controlled OFF switch allowed transcription to be started with one wavelength and translation of the corresponding mRNA to protein to be halted with a different wavelength at multiple timepoints. Derived from claim c4. Section: abstract. Quoted text: By combining with our previously generated blue-light-activated DNA template ON switch, we were able to start transcription with one wavelength of light and then halt the translation of the corresponding mRNA to protein with a different wavelength, at multiple timepoints.
Source:
successCell-freeapplication demo
Inferred from claim c4 during normalization. Combining the blue-light-activated DNA template ON switch with the light-controlled OFF switch allowed transcription to be started with one wavelength and translation of the corresponding mRNA to protein to be halted with a different wavelength at multiple timepoints. Derived from claim c4. Section: abstract. Quoted text: By combining with our previously generated blue-light-activated DNA template ON switch, we were able to start transcription with one wavelength of light and then halt the translation of the corresponding mRNA to protein with a different wavelength, at multiple timepoints.
Source:
successCell-freeapplication demo
Inferred from claim c4 during normalization. Combining the blue-light-activated DNA template ON switch with the light-controlled OFF switch allowed transcription to be started with one wavelength and translation of the corresponding mRNA to protein to be halted with a different wavelength at multiple timepoints. Derived from claim c4. Section: abstract. Quoted text: By combining with our previously generated blue-light-activated DNA template ON switch, we were able to start transcription with one wavelength of light and then halt the translation of the corresponding mRNA to protein with a different wavelength, at multiple timepoints.
Source:
successCell-freeapplication demo
Inferred from claim c4 during normalization. Combining the blue-light-activated DNA template ON switch with the light-controlled OFF switch allowed transcription to be started with one wavelength and translation of the corresponding mRNA to protein to be halted with a different wavelength at multiple timepoints. Derived from claim c4. Section: abstract. Quoted text: By combining with our previously generated blue-light-activated DNA template ON switch, we were able to start transcription with one wavelength of light and then halt the translation of the corresponding mRNA to protein with a different wavelength, at multiple timepoints.
Source:
successCell-freeapplication demo
Inferred from claim c4 during normalization. Combining the blue-light-activated DNA template ON switch with the light-controlled OFF switch allowed transcription to be started with one wavelength and translation of the corresponding mRNA to protein to be halted with a different wavelength at multiple timepoints. Derived from claim c4. Section: abstract. Quoted text: By combining with our previously generated blue-light-activated DNA template ON switch, we were able to start transcription with one wavelength of light and then halt the translation of the corresponding mRNA to protein with a different wavelength, at multiple timepoints.
Source:
Supporting Sources
Ranked Claims
Combining the blue-light-activated DNA template ON switch with the light-controlled OFF switch allowed transcription to be started with one wavelength and translation of the corresponding mRNA to protein to be halted with a different wavelength at multiple timepoints.
By combining with our previously generated blue-light-activated DNA template ON switch, we were able to start transcription with one wavelength of light and then halt the translation of the corresponding mRNA to protein with a different wavelength, at multiple timepoints.
Section: abstract
Combining the blue-light-activated DNA template ON switch with the light-controlled OFF switch allowed transcription to be started with one wavelength and translation of the corresponding mRNA to protein to be halted with a different wavelength at multiple timepoints.
By combining with our previously generated blue-light-activated DNA template ON switch, we were able to start transcription with one wavelength of light and then halt the translation of the corresponding mRNA to protein with a different wavelength, at multiple timepoints.
Section: abstract
Combining the blue-light-activated DNA template ON switch with the light-controlled OFF switch allowed transcription to be started with one wavelength and translation of the corresponding mRNA to protein to be halted with a different wavelength at multiple timepoints.
By combining with our previously generated blue-light-activated DNA template ON switch, we were able to start transcription with one wavelength of light and then halt the translation of the corresponding mRNA to protein with a different wavelength, at multiple timepoints.
Section: abstract
Combining the blue-light-activated DNA template ON switch with the light-controlled OFF switch allowed transcription to be started with one wavelength and translation of the corresponding mRNA to protein to be halted with a different wavelength at multiple timepoints.
By combining with our previously generated blue-light-activated DNA template ON switch, we were able to start transcription with one wavelength of light and then halt the translation of the corresponding mRNA to protein with a different wavelength, at multiple timepoints.
Section: abstract
Combining the blue-light-activated DNA template ON switch with the light-controlled OFF switch allowed transcription to be started with one wavelength and translation of the corresponding mRNA to protein to be halted with a different wavelength at multiple timepoints.
By combining with our previously generated blue-light-activated DNA template ON switch, we were able to start transcription with one wavelength of light and then halt the translation of the corresponding mRNA to protein with a different wavelength, at multiple timepoints.
Section: abstract
Combining the blue-light-activated DNA template ON switch with the light-controlled OFF switch allowed transcription to be started with one wavelength and translation of the corresponding mRNA to protein to be halted with a different wavelength at multiple timepoints.
By combining with our previously generated blue-light-activated DNA template ON switch, we were able to start transcription with one wavelength of light and then halt the translation of the corresponding mRNA to protein with a different wavelength, at multiple timepoints.
Section: abstract
Combining the blue-light-activated DNA template ON switch with the light-controlled OFF switch allowed transcription to be started with one wavelength and translation of the corresponding mRNA to protein to be halted with a different wavelength at multiple timepoints.
By combining with our previously generated blue-light-activated DNA template ON switch, we were able to start transcription with one wavelength of light and then halt the translation of the corresponding mRNA to protein with a different wavelength, at multiple timepoints.
Section: abstract
Approval Evidence
1 source1 linked approval claimfirst-pass slug blue-light-activated-dna-template-on-switch
By combining with our previously generated blue-light-activated DNA template ON switch, we were able to start transcription with one wavelength of light and then halt the translation of the corresponding mRNA to protein with a different wavelength, at multiple timepoints.
Source:
combined controlsupports
Combining the blue-light-activated DNA template ON switch with the light-controlled OFF switch allowed transcription to be started with one wavelength and translation of the corresponding mRNA to protein to be halted with a different wavelength at multiple timepoints.
By combining with our previously generated blue-light-activated DNA template ON switch, we were able to start transcription with one wavelength of light and then halt the translation of the corresponding mRNA to protein with a different wavelength, at multiple timepoints.
Source:
Comparisons
Source-backed strengths
The main demonstrated strength is orthogonal wavelength-dependent control in a combined system, where transcription was initiated with one wavelength and translation was halted with another. The study also reports temporal flexibility, as translation could be stopped at multiple timepoints after transcription had been started.
Compared with 4pLRE-cPAOX1
blue-light-activated DNA template ON switch and 4pLRE-cPAOX1 address a similar problem space because they share transcription, translation.
Shared frame: same top-level item type; shared target processes: transcription, translation; shared mechanisms: translation_control; same primary input modality: light
Compared with PGAP-pLRE
blue-light-activated DNA template ON switch and PGAP-pLRE address a similar problem space because they share transcription, translation.
Shared frame: same top-level item type; shared target processes: transcription, translation; shared mechanisms: translation_control; same primary input modality: light
Compared with triple brake design
blue-light-activated DNA template ON switch and triple brake design address a similar problem space because they share transcription, translation.
Shared frame: same top-level item type; shared target processes: transcription, translation; shared mechanisms: translation_control; same primary input modality: light
Ranked Citations
- 1.