Source 1review2013Chemical Society Reviews
Toolkit/BphP-based biosensors
BphP-based biosensors
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
BphP-based biosensors are proposed near-infrared optical sensor designs derived from bacterial phytochrome photoreceptors. They are suggested on the basis of bacterial phytochrome photochemistry and structure as a possible class of genetically encoded biosensors spectrally complementary to other probes.
Usefulness & Problems
Why this is useful
These proposed biosensors are useful in principle because near-infrared light is favorable for imaging in mammalian tissues, where hemoglobin, melanin, and water have low absorbance. The concept therefore addresses the need for genetically encoded sensors with spectra better matched to mammalian tissue optical windows.
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This refers to biosensor designs built from bacterial phytochrome photoreceptors for near-infrared optical readout. The abstract presents them as a suggested class of possible BphP-based tools.
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optical readout in mammals
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near-infrared sensing in mammalian tissues
Problem solved
The specific problem addressed is the limited suitability of shorter-wavelength optical probes for deep or tissue-compatible imaging in mammals. BphP-based biosensors are proposed to provide near-infrared sensor architectures based on bacterial phytochrome photochemical and structural properties.
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They aim to provide biosensors with spectra better matched to mammalian tissue optical windows.
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enabling biosensing with optical properties in the near-infrared window
Published Workflows
Objective: Engineer bacterial phytochrome-based optical tools for mammalian imaging, sensing, and light control in the near-infrared window.
Why it works: The review links the mammalian near-infrared optical window to BphP suitability, noting that BphPs absorb in the near-infrared and use biliverdin present in most mammalian tissues.
biliverdin-dependent photoreactivityBphP photochemistryBphP structural tuningtemplate selectionphotochemistry analysisstructure-guided design
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level protein part used inside a larger architecture that realizes a mechanism.
Techniques
Computational DesignTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The designs are described as relying on bacterial phytochrome photochemistry and structure, and the extraction notes biliverdin as the chromophore. The supplied evidence does not provide construct architecture, host expression details, or assay protocols for specific biosensor implementations.
The evidence describes these biosensors as suggested or possible designs rather than experimentally validated tools. The available text does not specify sensing modalities, dynamic range, kinetics, specificity, or demonstrated in vivo performance.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Near-infrared light is favorable for imaging in mammalian tissues because hemoglobin, melanin, and water have low absorbance in this range.
Near-infrared light is favorable for imaging in mammalian tissues because hemoglobin, melanin, and water have low absorbance in this range.
Near-infrared light is favorable for imaging in mammalian tissues because hemoglobin, melanin, and water have low absorbance in this range.
Near-infrared light is favorable for imaging in mammalian tissues because hemoglobin, melanin, and water have low absorbance in this range.
Near-infrared light is favorable for imaging in mammalian tissues because hemoglobin, melanin, and water have low absorbance in this range.
Near-infrared light is favorable for imaging in mammalian tissues because hemoglobin, melanin, and water have low absorbance in this range.
Near-infrared light is favorable for imaging in mammalian tissues because hemoglobin, melanin, and water have low absorbance in this range.
Near-infrared light is favorable for imaging in mammalian tissues because hemoglobin, melanin, and water have low absorbance in this range.
Near-infrared light is favorable for imaging in mammalian tissues because hemoglobin, melanin, and water have low absorbance in this range.
Optical probes and optogenetic constructs intended for mammalian imaging, readout, and light manipulation should have fluorescence and action spectra within the near-infrared window.
Optical probes and optogenetic constructs intended for mammalian imaging, readout, and light manipulation should have fluorescence and action spectra within the near-infrared window.
Optical probes and optogenetic constructs intended for mammalian imaging, readout, and light manipulation should have fluorescence and action spectra within the near-infrared window.
Optical probes and optogenetic constructs intended for mammalian imaging, readout, and light manipulation should have fluorescence and action spectra within the near-infrared window.
Optical probes and optogenetic constructs intended for mammalian imaging, readout, and light manipulation should have fluorescence and action spectra within the near-infrared window.
Optical probes and optogenetic constructs intended for mammalian imaging, readout, and light manipulation should have fluorescence and action spectra within the near-infrared window.
Optical probes and optogenetic constructs intended for mammalian imaging, readout, and light manipulation should have fluorescence and action spectra within the near-infrared window.
Optical probes and optogenetic constructs intended for mammalian imaging, readout, and light manipulation should have fluorescence and action spectra within the near-infrared window.
Optical probes and optogenetic constructs intended for mammalian imaging, readout, and light manipulation should have fluorescence and action spectra within the near-infrared window.
Bacterial phytochrome photoreceptors use biliverdin, a chromophore found in most mammalian tissues, which supports their use as templates for mammalian optical tools.
Bacterial phytochrome photoreceptors use biliverdin, a chromophore found in most mammalian tissues, which supports their use as templates for mammalian optical tools.
Bacterial phytochrome photoreceptors use biliverdin, a chromophore found in most mammalian tissues, which supports their use as templates for mammalian optical tools.
Bacterial phytochrome photoreceptors use biliverdin, a chromophore found in most mammalian tissues, which supports their use as templates for mammalian optical tools.
Bacterial phytochrome photoreceptors use biliverdin, a chromophore found in most mammalian tissues, which supports their use as templates for mammalian optical tools.
Bacterial phytochrome photoreceptors use biliverdin, a chromophore found in most mammalian tissues, which supports their use as templates for mammalian optical tools.
Bacterial phytochrome photoreceptors use biliverdin, a chromophore found in most mammalian tissues, which supports their use as templates for mammalian optical tools.
Bacterial phytochrome photoreceptors use biliverdin, a chromophore found in most mammalian tissues, which supports their use as templates for mammalian optical tools.
Bacterial phytochrome photoreceptors use biliverdin, a chromophore found in most mammalian tissues, which supports their use as templates for mammalian optical tools.
BphPs spectrally complement existing genetically encoded probes.
BphPs spectrally complement existing genetically encoded probes.
BphPs spectrally complement existing genetically encoded probes.
BphPs spectrally complement existing genetically encoded probes.
BphPs spectrally complement existing genetically encoded probes.
BphPs spectrally complement existing genetically encoded probes.
BphPs spectrally complement existing genetically encoded probes.
BphPs spectrally complement existing genetically encoded probes.
BphPs spectrally complement existing genetically encoded probes.
Because of their near-infrared absorbance, BphPs are preferred templates for designing optical molecular tools for applications in mammals.
Because of their near-infrared absorbance, BphPs are preferred templates for designing optical molecular tools for applications in mammals.
Because of their near-infrared absorbance, BphPs are preferred templates for designing optical molecular tools for applications in mammals.
Because of their near-infrared absorbance, BphPs are preferred templates for designing optical molecular tools for applications in mammals.
Because of their near-infrared absorbance, BphPs are preferred templates for designing optical molecular tools for applications in mammals.
Because of their near-infrared absorbance, BphPs are preferred templates for designing optical molecular tools for applications in mammals.
Because of their near-infrared absorbance, BphPs are preferred templates for designing optical molecular tools for applications in mammals.
Because of their near-infrared absorbance, BphPs are preferred templates for designing optical molecular tools for applications in mammals.
Because of their near-infrared absorbance, BphPs are preferred templates for designing optical molecular tools for applications in mammals.
Approval Evidence
1 source5 linked approval claimsfirst-pass slug bphp-based-biosensors
Based on the analysis of the photochemistry and structure of BphPs we suggest a variety of possible BphP-based fluorescent proteins, biosensors, and optogenetic tools.
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application rationalesupports
Near-infrared light is favorable for imaging in mammalian tissues because hemoglobin, melanin, and water have low absorbance in this range.
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design principlesupports
Optical probes and optogenetic constructs intended for mammalian imaging, readout, and light manipulation should have fluorescence and action spectra within the near-infrared window.
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mechanistic enablersupports
Bacterial phytochrome photoreceptors use biliverdin, a chromophore found in most mammalian tissues, which supports their use as templates for mammalian optical tools.
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spectral complementaritysupports
BphPs spectrally complement existing genetically encoded probes.
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template preferencesupports
Because of their near-infrared absorbance, BphPs are preferred templates for designing optical molecular tools for applications in mammals.
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Comparisons
Source-backed strengths
A key proposed advantage is spectral operation in the near-infrared range, which is favorable for mammalian tissue imaging because endogenous absorbers have low absorbance there. The source also indicates that bacterial phytochrome photochemistry and structure provide a basis for designing a variety of possible biosensors.
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BphPs are preferred templates for designing optical molecular tools for applications in mammals
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BphPs use biliverdin found in most mammalian tissues
Ranked Citations
- 1.