Source 1primary paper2018ChemBioChem
Toolkit/BphP1-QPAS1
BphP1-QPAS1
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
BphP1-QPAS1 is a near-infrared light-inducible protein interaction system in which the bacterial phytochrome BphP1 binds an engineered partner, QPAS1, for optical protein regulation in mammalian cells. It has been incorporated into multi-component optogenetic tools for transcriptional control and protein targeting, including use in neurons and non-neuronal cells.
Usefulness & Problems
Why this is useful
This system enables optical regulation with near-infrared light in mammalian cells, supporting control of protein targeting and gene expression. The small size of QPAS1 also enabled adeno-associated virus particle design for neuronal delivery of a BphP1-QPAS1-based transcriptional activation system.
Source:
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Problem solved
BphP1-QPAS1 helps solve the problem of controlling protein localization and transcription in mammalian cells with a near-infrared light-inducible interaction module. It also addresses packaging constraints for neuronal delivery in at least one implementation because QPAS1 is small enough to support AAV-based design.
Source:
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
light-inducible heterodimerizationoptical control of protein localizationoptical control of transcriptionTechniques
Computational DesignTarget processes
localizationtranscriptionInput: Light
Implementation Constraints
BphP1 functions with an engineered binding partner, QPAS1, in a multi-component optogenetic configuration for mammalian cells. Practical implementation evidence includes AAV particle design for neuronal delivery of the TA system enabled by the small size of QPAS1, but the supplied evidence does not provide further construct architecture or cofactor details.
Performance depended on physiological properties of specific cell types, including nuclear transport. The cited evidence also notes that applicability of the blue-light-sensitive component of iRIS could be limited, indicating context dependence in multi-component implementations.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.
We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.
We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.
We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.
We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.
We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.
We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.
We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
The NIR-light-sensing component of iRIS performed well in all tested cell types.
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
The NIR-light-sensing component of iRIS performed well in all tested cell types.
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
The NIR-light-sensing component of iRIS performed well in all tested cell types.
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
The NIR-light-sensing component of iRIS performed well in all tested cell types.
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
The NIR-light-sensing component of iRIS performed well in all tested cell types.
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
The NIR-light-sensing component of iRIS performed well in all tested cell types.
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
The NIR-light-sensing component of iRIS performed well in all tested cell types.
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug bphp1-qpas1
Near-infrared (NIR) light-inducible binding of bacterial phytochrome BphP1 to its engineered partner, QPAS1, is used for optical protein regulation in mammalian cells.
Source:
application scopesupports
The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.
Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Source:
delivery enabling propertysupports
The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.
The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
Source:
Comparisons
Source-backed strengths
The system was tested in several mammalian cell types, including cortical neurons, through two BphP1-QPAS1-based tools, iRIS and the TA system. Its demonstrated use for both protein targeting and transcriptional control indicates functional versatility across multiple cellular contexts.
Source:
In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Source:
The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Ranked Citations
- 1.