Source 1primary paper2020
Toolkit/C120 promoter
C120 promoter
Also known as: C120 regulatory element, TAEL-responsive C120 promoter
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The C120 promoter is a TAEL-responsive regulatory element used in the zebrafish TAEL optogenetic transcription system. Blue light induces TAEL transcription factor dimerization, which activates transcription from the C120 promoter.
Usefulness & Problems
Why this is useful
This promoter enables light-gated control of transcription when paired with the TAEL transcription factor system. In the TAEL 2.0 context, it supports inducible expression at late embryonic and larval stages through functional stable transgenic lines.
Source:
We demonstrate that the ubiquitous line in particular can be used to induce expression at late embryonic and larval stages, addressing a major deficiency of the original TAEL system.
Problem solved
The C120 promoter helps solve the problem of achieving temporally controlled, blue-light-inducible gene expression in zebrafish. In the reported TAEL 2.0 system, it contributes to overcoming a major deficiency of the original TAEL system by supporting inducible expression at later developmental stages.
Source:
We demonstrate that the ubiquitous line in particular can be used to induce expression at late embryonic and larval stages, addressing a major deficiency of the original TAEL system.
Problem links
Need precise spatiotemporal control with light input
DerivedThe C120 promoter is a TAEL-responsive regulatory element used in a light-gated transcription system. In the reported zebrafish TAEL system, blue light induces TAEL transcription factor dimerization, which activates gene expression downstream of the C120 promoter.
Need tighter control over gene expression timing or amplitude
DerivedThe C120 promoter is a TAEL-responsive regulatory element used in a light-gated transcription system. In the reported zebrafish TAEL system, blue light induces TAEL transcription factor dimerization, which activates gene expression downstream of the C120 promoter.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
dimerization-dependent transcriptional activationHeterodimerizationHeterodimerizationlight-activated transcriptionlight-activated transcriptionTechniques
No technique tags yet.
Target processes
transcriptionInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementoperating role: regulatoroperating role: reporterswitch architecture: multi componentswitch architecture: recruitment
C120 functions downstream of the TAEL transcription factor and requires blue-light illumination to drive TAEL dimerization-dependent transcriptional activation. Reported implementations used stable zebrafish transgenic lines expressing TAEL 2.0 either ubiquitously or from a tissue-specific promoter.
The supplied evidence describes C120 only as a TAEL-responsive promoter element within the zebrafish TAEL 2.0 system and does not provide promoter sequence, basal activity measurements, or cross-species validation. Independent replication beyond the cited 2020 study is not provided.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
A ubiquitous TAEL 2.0 transgenic line can induce expression at late embryonic and larval stages, addressing a major deficiency of the original TAEL system.
We demonstrate that the ubiquitous line in particular can be used to induce expression at late embryonic and larval stages, addressing a major deficiency of the original TAEL system.
A ubiquitous TAEL 2.0 transgenic line can induce expression at late embryonic and larval stages, addressing a major deficiency of the original TAEL system.
We demonstrate that the ubiquitous line in particular can be used to induce expression at late embryonic and larval stages, addressing a major deficiency of the original TAEL system.
A ubiquitous TAEL 2.0 transgenic line can induce expression at late embryonic and larval stages, addressing a major deficiency of the original TAEL system.
We demonstrate that the ubiquitous line in particular can be used to induce expression at late embryonic and larval stages, addressing a major deficiency of the original TAEL system.
A ubiquitous TAEL 2.0 transgenic line can induce expression at late embryonic and larval stages, addressing a major deficiency of the original TAEL system.
We demonstrate that the ubiquitous line in particular can be used to induce expression at late embryonic and larval stages, addressing a major deficiency of the original TAEL system.
A ubiquitous TAEL 2.0 transgenic line can induce expression at late embryonic and larval stages, addressing a major deficiency of the original TAEL system.
We demonstrate that the ubiquitous line in particular can be used to induce expression at late embryonic and larval stages, addressing a major deficiency of the original TAEL system.
The improvements in TAEL 2.0 enabled creation of functional stable transgenic lines expressing the TAEL 2.0 transcription factor either ubiquitously or with a tissue-specific promoter.
With these improvements, we were able to create functional stable transgenic lines to express the TAEL 2.0 transcription factor either ubiquitously or with a tissue-specific promoter.
The improvements in TAEL 2.0 enabled creation of functional stable transgenic lines expressing the TAEL 2.0 transcription factor either ubiquitously or with a tissue-specific promoter.
With these improvements, we were able to create functional stable transgenic lines to express the TAEL 2.0 transcription factor either ubiquitously or with a tissue-specific promoter.
The improvements in TAEL 2.0 enabled creation of functional stable transgenic lines expressing the TAEL 2.0 transcription factor either ubiquitously or with a tissue-specific promoter.
With these improvements, we were able to create functional stable transgenic lines to express the TAEL 2.0 transcription factor either ubiquitously or with a tissue-specific promoter.
The improvements in TAEL 2.0 enabled creation of functional stable transgenic lines expressing the TAEL 2.0 transcription factor either ubiquitously or with a tissue-specific promoter.
With these improvements, we were able to create functional stable transgenic lines to express the TAEL 2.0 transcription factor either ubiquitously or with a tissue-specific promoter.
The improvements in TAEL 2.0 enabled creation of functional stable transgenic lines expressing the TAEL 2.0 transcription factor either ubiquitously or with a tissue-specific promoter.
With these improvements, we were able to create functional stable transgenic lines to express the TAEL 2.0 transcription factor either ubiquitously or with a tissue-specific promoter.
Blue light causes TAEL transcription factors to dimerize and activate gene expression downstream of the C120 promoter.
When illuminated with blue light, TAEL transcription factors dimerize and activate gene expression downstream of the TAEL-responsive C120 promoter.
Blue light causes TAEL transcription factors to dimerize and activate gene expression downstream of the C120 promoter.
When illuminated with blue light, TAEL transcription factors dimerize and activate gene expression downstream of the TAEL-responsive C120 promoter.
Blue light causes TAEL transcription factors to dimerize and activate gene expression downstream of the C120 promoter.
When illuminated with blue light, TAEL transcription factors dimerize and activate gene expression downstream of the TAEL-responsive C120 promoter.
Blue light causes TAEL transcription factors to dimerize and activate gene expression downstream of the C120 promoter.
When illuminated with blue light, TAEL transcription factors dimerize and activate gene expression downstream of the TAEL-responsive C120 promoter.
Blue light causes TAEL transcription factors to dimerize and activate gene expression downstream of the C120 promoter.
When illuminated with blue light, TAEL transcription factors dimerize and activate gene expression downstream of the TAEL-responsive C120 promoter.
Blue light causes TAEL transcription factors to dimerize and activate gene expression downstream of the C120 promoter.
When illuminated with blue light, TAEL transcription factors dimerize and activate gene expression downstream of the TAEL-responsive C120 promoter.
Blue light causes TAEL transcription factors to dimerize and activate gene expression downstream of the C120 promoter.
When illuminated with blue light, TAEL transcription factors dimerize and activate gene expression downstream of the TAEL-responsive C120 promoter.
Blue light causes TAEL transcription factors to dimerize and activate gene expression downstream of the C120 promoter.
When illuminated with blue light, TAEL transcription factors dimerize and activate gene expression downstream of the TAEL-responsive C120 promoter.
Blue light causes TAEL transcription factors to dimerize and activate gene expression downstream of the C120 promoter.
When illuminated with blue light, TAEL transcription factors dimerize and activate gene expression downstream of the TAEL-responsive C120 promoter.
Blue light causes TAEL transcription factors to dimerize and activate gene expression downstream of the C120 promoter.
When illuminated with blue light, TAEL transcription factors dimerize and activate gene expression downstream of the TAEL-responsive C120 promoter.
Blue light causes TAEL transcription factors to dimerize and activate gene expression downstream of the C120 promoter.
When illuminated with blue light, TAEL transcription factors dimerize and activate gene expression downstream of the TAEL-responsive C120 promoter.
Blue light causes TAEL transcription factors to dimerize and activate gene expression downstream of the C120 promoter.
When illuminated with blue light, TAEL transcription factors dimerize and activate gene expression downstream of the TAEL-responsive C120 promoter.
Blue light causes TAEL transcription factors to dimerize and activate gene expression downstream of the C120 promoter.
When illuminated with blue light, TAEL transcription factors dimerize and activate gene expression downstream of the TAEL-responsive C120 promoter.
Blue light causes TAEL transcription factors to dimerize and activate gene expression downstream of the C120 promoter.
When illuminated with blue light, TAEL transcription factors dimerize and activate gene expression downstream of the TAEL-responsive C120 promoter.
Blue light causes TAEL transcription factors to dimerize and activate gene expression downstream of the C120 promoter.
When illuminated with blue light, TAEL transcription factors dimerize and activate gene expression downstream of the TAEL-responsive C120 promoter.
Blue light causes TAEL transcription factors to dimerize and activate gene expression downstream of the C120 promoter.
When illuminated with blue light, TAEL transcription factors dimerize and activate gene expression downstream of the TAEL-responsive C120 promoter.
Blue light causes TAEL transcription factors to dimerize and activate gene expression downstream of the C120 promoter.
When illuminated with blue light, TAEL transcription factors dimerize and activate gene expression downstream of the TAEL-responsive C120 promoter.
TAEL 2.0 induces higher levels of reporter gene expression and does so faster than the original TAEL system, while maintaining comparable background and toxicity.
We demonstrate that TAEL 2.0 consistently induces higher levels of reporter gene expression and at a faster rate, but with comparable background and toxicity as the original TAEL system.
TAEL 2.0 induces higher levels of reporter gene expression and does so faster than the original TAEL system, while maintaining comparable background and toxicity.
We demonstrate that TAEL 2.0 consistently induces higher levels of reporter gene expression and at a faster rate, but with comparable background and toxicity as the original TAEL system.
TAEL 2.0 induces higher levels of reporter gene expression and does so faster than the original TAEL system, while maintaining comparable background and toxicity.
We demonstrate that TAEL 2.0 consistently induces higher levels of reporter gene expression and at a faster rate, but with comparable background and toxicity as the original TAEL system.
TAEL 2.0 induces higher levels of reporter gene expression and does so faster than the original TAEL system, while maintaining comparable background and toxicity.
We demonstrate that TAEL 2.0 consistently induces higher levels of reporter gene expression and at a faster rate, but with comparable background and toxicity as the original TAEL system.
TAEL 2.0 induces higher levels of reporter gene expression and does so faster than the original TAEL system, while maintaining comparable background and toxicity.
We demonstrate that TAEL 2.0 consistently induces higher levels of reporter gene expression and at a faster rate, but with comparable background and toxicity as the original TAEL system.
Approval Evidence
1 source1 linked approval claimfirst-pass slug c120-promoter
TAEL transcription factors dimerize and activate gene expression downstream of the TAEL-responsive C120 promoter.
Source:
mechanismsupports
Blue light causes TAEL transcription factors to dimerize and activate gene expression downstream of the C120 promoter.
When illuminated with blue light, TAEL transcription factors dimerize and activate gene expression downstream of the TAEL-responsive C120 promoter.
Source:
Comparisons
Source-backed strengths
Within TAEL 2.0, C120-driven reporter expression was induced faster and to higher levels than in the original TAEL system, while maintaining comparable background and toxicity. The system was implemented in stable transgenic lines with either ubiquitous or tissue-specific TAEL 2.0 expression.
Source:
With these improvements, we were able to create functional stable transgenic lines to express the TAEL 2.0 transcription factor either ubiquitously or with a tissue-specific promoter.
Source:
We demonstrate that TAEL 2.0 consistently induces higher levels of reporter gene expression and at a faster rate, but with comparable background and toxicity as the original TAEL system.
Compared with iLID/SspB
C120 promoter and iLID/SspB address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; shared mechanisms: heterodimerization; same primary input modality: light
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Compared with LITEs (Light-inducible transcriptional effectors)
C120 promoter and LITEs (Light-inducible transcriptional effectors) address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; shared mechanisms: heterodimerization; same primary input modality: light
Compared with LOVpep/ePDZb
C120 promoter and LOVpep/ePDZb address a similar problem space because they share transcription.
Shared frame: same top-level item type; shared target processes: transcription; shared mechanisms: heterodimerization; same primary input modality: light
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Ranked Citations
- 1.