Toolkit/Ca2+/cAMP response element decoy oligodeoxynucleotide

Ca2+/cAMP response element decoy oligodeoxynucleotide

Multi-Component Switch·Research·Since 2003

Also known as: Ca(2+)/cAMP response element (CRE)-decoy ODN, CRE-decoy ODN

Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.

Summary

The Ca2+/cAMP response element decoy oligodeoxynucleotide (CRE-decoy ODN) is an oligodeoxynucleotide perturbation reagent used in the rat suprachiasmatic nucleus to interfere with CREB-dependent transcription. In the cited study, it blocked glutamate-induced Per1 mRNA accumulation and prevented glutamate-induced phase advances of the circadian clock, implicating CREB-dependent Per1 activation in light-responsive circadian signaling.

Usefulness & Problems

Why this is useful

This reagent is useful for testing whether transcription through a Ca2+/cAMP response element is required in a defined signaling pathway. In the reported rat SCN experiments, it enabled functional disruption of CREB-dependent transcription linking glutamatergic input to Per1 induction and circadian phase shifting.

Problem solved

It addresses the problem of causally perturbing CREB-dependent gene regulation in the suprachiasmatic nucleus without directly altering the CREB protein or the Per1 locus. The cited work used it to determine whether CRE-mediated transcription is necessary for glutamate-triggered Per1 expression and clock phase advance.

Taxonomy & Function

Primary hierarchy

Mechanism Branch

Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.

Techniques

No technique tags yet.

Target processes

recombinationsignaling

Input: Light

Implementation Constraints

The tool is an oligodeoxynucleotide decoy directed against the Ca2+/cAMP response element and was applied in rat SCN experiments involving glutamatergic stimulation. The supplied evidence does not specify the ODN sequence, chemical modifications, delivery method, or whether exogenous cofactors were required.

The evidence provided comes from a single cited study in rat suprachiasmatic nucleus tissue, so validation breadth is limited. The supplied evidence does not report sequence design, delivery conditions, off-target assessment, kinetics, dose response, or performance in other cell types or organisms.

Validation

Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication

Observations

successMouseapplication demoratsuprachiasmatic nucleus

Inferred from claim c3 during normalization. CRE-decoy oligodeoxynucleotide blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus. Derived from claim c3. Section: abstract. Quoted text: Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.

Source:

successMouseapplication demoratsuprachiasmatic nucleus

Inferred from claim c3 during normalization. CRE-decoy oligodeoxynucleotide blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus. Derived from claim c3. Section: abstract. Quoted text: Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.

Source:

successMouseapplication demoratsuprachiasmatic nucleus

Inferred from claim c3 during normalization. CRE-decoy oligodeoxynucleotide blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus. Derived from claim c3. Section: abstract. Quoted text: Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.

Source:

successMouseapplication demoratsuprachiasmatic nucleus

Inferred from claim c3 during normalization. CRE-decoy oligodeoxynucleotide blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus. Derived from claim c3. Section: abstract. Quoted text: Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.

Source:

successMouseapplication demoratsuprachiasmatic nucleus

Inferred from claim c3 during normalization. CRE-decoy oligodeoxynucleotide blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus. Derived from claim c3. Section: abstract. Quoted text: Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.

Source:

successMouseapplication demoratsuprachiasmatic nucleus

Inferred from claim c3 during normalization. CRE-decoy oligodeoxynucleotide blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus. Derived from claim c3. Section: abstract. Quoted text: Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.

Source:

successMouseapplication demoratsuprachiasmatic nucleus

Inferred from claim c3 during normalization. CRE-decoy oligodeoxynucleotide blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus. Derived from claim c3. Section: abstract. Quoted text: Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.

Source:

Supporting Sources

Ranked Claims

Claim 1gene expression effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced accumulation of Per1 mRNA.

Furthermore, the CRE-decoy blocked GLU-induced accumulation of Per1 mRNA.
Section: abstract
Claim 2gene expression effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced accumulation of Per1 mRNA.

Furthermore, the CRE-decoy blocked GLU-induced accumulation of Per1 mRNA.
Section: abstract
Claim 3gene expression effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced accumulation of Per1 mRNA.

Furthermore, the CRE-decoy blocked GLU-induced accumulation of Per1 mRNA.
Section: abstract
Claim 4gene expression effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced accumulation of Per1 mRNA.

Furthermore, the CRE-decoy blocked GLU-induced accumulation of Per1 mRNA.
Section: abstract
Claim 5gene expression effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced accumulation of Per1 mRNA.

Furthermore, the CRE-decoy blocked GLU-induced accumulation of Per1 mRNA.
Section: abstract
Claim 6gene expression effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced accumulation of Per1 mRNA.

Furthermore, the CRE-decoy blocked GLU-induced accumulation of Per1 mRNA.
Section: abstract
Claim 7gene expression effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced accumulation of Per1 mRNA.

Furthermore, the CRE-decoy blocked GLU-induced accumulation of Per1 mRNA.
Section: abstract
Claim 8pathway component rolesupports2003Source 1needs review

CREB and Per1 are integral components of the pathway that transduces light-stimulated glutamatergic neurotransmission into phase advance of the circadian clock.

Thus, Ca(2+)/cAMP response element-binding protein (CREB) and Per1 are integral components of the pathway transducing light-stimulated GLU neurotransmission into phase advance of the circadian clock.
Section: abstract
Claim 9pathway component rolesupports2003Source 1needs review

CREB and Per1 are integral components of the pathway that transduces light-stimulated glutamatergic neurotransmission into phase advance of the circadian clock.

Thus, Ca(2+)/cAMP response element-binding protein (CREB) and Per1 are integral components of the pathway transducing light-stimulated GLU neurotransmission into phase advance of the circadian clock.
Section: abstract
Claim 10pathway component rolesupports2003Source 1needs review

CREB and Per1 are integral components of the pathway that transduces light-stimulated glutamatergic neurotransmission into phase advance of the circadian clock.

Thus, Ca(2+)/cAMP response element-binding protein (CREB) and Per1 are integral components of the pathway transducing light-stimulated GLU neurotransmission into phase advance of the circadian clock.
Section: abstract
Claim 11pathway component rolesupports2003Source 1needs review

CREB and Per1 are integral components of the pathway that transduces light-stimulated glutamatergic neurotransmission into phase advance of the circadian clock.

Thus, Ca(2+)/cAMP response element-binding protein (CREB) and Per1 are integral components of the pathway transducing light-stimulated GLU neurotransmission into phase advance of the circadian clock.
Section: abstract
Claim 12pathway component rolesupports2003Source 1needs review

CREB and Per1 are integral components of the pathway that transduces light-stimulated glutamatergic neurotransmission into phase advance of the circadian clock.

Thus, Ca(2+)/cAMP response element-binding protein (CREB) and Per1 are integral components of the pathway transducing light-stimulated GLU neurotransmission into phase advance of the circadian clock.
Section: abstract
Claim 13pathway component rolesupports2003Source 1needs review

CREB and Per1 are integral components of the pathway that transduces light-stimulated glutamatergic neurotransmission into phase advance of the circadian clock.

Thus, Ca(2+)/cAMP response element-binding protein (CREB) and Per1 are integral components of the pathway transducing light-stimulated GLU neurotransmission into phase advance of the circadian clock.
Section: abstract
Claim 14pathway component rolesupports2003Source 1needs review

CREB and Per1 are integral components of the pathway that transduces light-stimulated glutamatergic neurotransmission into phase advance of the circadian clock.

Thus, Ca(2+)/cAMP response element-binding protein (CREB) and Per1 are integral components of the pathway transducing light-stimulated GLU neurotransmission into phase advance of the circadian clock.
Section: abstract
Claim 15perturbation effectsupports2003Source 1needs review

Antisense oligodeoxynucleotide against Per1 blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1
Section: abstract
Claim 16perturbation effectsupports2003Source 1needs review

Antisense oligodeoxynucleotide against Per1 blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1
Section: abstract
Claim 17perturbation effectsupports2003Source 1needs review

Antisense oligodeoxynucleotide against Per1 blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1
Section: abstract
Claim 18perturbation effectsupports2003Source 1needs review

Antisense oligodeoxynucleotide against Per1 blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1
Section: abstract
Claim 19perturbation effectsupports2003Source 1needs review

Antisense oligodeoxynucleotide against Per1 blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1
Section: abstract
Claim 20perturbation effectsupports2003Source 1needs review

Antisense oligodeoxynucleotide against Per1 blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1
Section: abstract
Claim 21perturbation effectsupports2003Source 1needs review

Antisense oligodeoxynucleotide against Per1 blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1
Section: abstract
Claim 22perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.
Section: abstract
Claim 23perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.
Section: abstract
Claim 24perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.
Section: abstract
Claim 25perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.
Section: abstract
Claim 26perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.
Section: abstract
Claim 27perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.
Section: abstract
Claim 28perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.
Section: abstract
Claim 29perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks light-induced phase advances in vivo.

CRE-decoy ODN also blocked light-induced phase advances in vivo.
Section: abstract
Claim 30perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks light-induced phase advances in vivo.

CRE-decoy ODN also blocked light-induced phase advances in vivo.
Section: abstract
Claim 31perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks light-induced phase advances in vivo.

CRE-decoy ODN also blocked light-induced phase advances in vivo.
Section: abstract
Claim 32perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks light-induced phase advances in vivo.

CRE-decoy ODN also blocked light-induced phase advances in vivo.
Section: abstract
Claim 33perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks light-induced phase advances in vivo.

CRE-decoy ODN also blocked light-induced phase advances in vivo.
Section: abstract
Claim 34perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks light-induced phase advances in vivo.

CRE-decoy ODN also blocked light-induced phase advances in vivo.
Section: abstract
Claim 35perturbation effectsupports2003Source 1needs review

CRE-decoy oligodeoxynucleotide blocks light-induced phase advances in vivo.

CRE-decoy ODN also blocked light-induced phase advances in vivo.
Section: abstract
Claim 36requirement for signalingsupports2003Source 1needs review

CREB-dependent activation of Per1 is required for light-induced signaling in the suprachiasmatic nucleus circadian clock.

Ca2+/cAMP Response Element-binding Protein (CREB)-dependent Activation of Per1 Is Required for Light-induced Signaling in the Suprachiasmatic Nucleus Circadian Clock
Section: title/abstract
Claim 37requirement for signalingsupports2003Source 1needs review

CREB-dependent activation of Per1 is required for light-induced signaling in the suprachiasmatic nucleus circadian clock.

Ca2+/cAMP Response Element-binding Protein (CREB)-dependent Activation of Per1 Is Required for Light-induced Signaling in the Suprachiasmatic Nucleus Circadian Clock
Section: title/abstract
Claim 38requirement for signalingsupports2003Source 1needs review

CREB-dependent activation of Per1 is required for light-induced signaling in the suprachiasmatic nucleus circadian clock.

Ca2+/cAMP Response Element-binding Protein (CREB)-dependent Activation of Per1 Is Required for Light-induced Signaling in the Suprachiasmatic Nucleus Circadian Clock
Section: title/abstract
Claim 39requirement for signalingsupports2003Source 1needs review

CREB-dependent activation of Per1 is required for light-induced signaling in the suprachiasmatic nucleus circadian clock.

Ca2+/cAMP Response Element-binding Protein (CREB)-dependent Activation of Per1 Is Required for Light-induced Signaling in the Suprachiasmatic Nucleus Circadian Clock
Section: title/abstract
Claim 40requirement for signalingsupports2003Source 1needs review

CREB-dependent activation of Per1 is required for light-induced signaling in the suprachiasmatic nucleus circadian clock.

Ca2+/cAMP Response Element-binding Protein (CREB)-dependent Activation of Per1 Is Required for Light-induced Signaling in the Suprachiasmatic Nucleus Circadian Clock
Section: title/abstract
Claim 41requirement for signalingsupports2003Source 1needs review

CREB-dependent activation of Per1 is required for light-induced signaling in the suprachiasmatic nucleus circadian clock.

Ca2+/cAMP Response Element-binding Protein (CREB)-dependent Activation of Per1 Is Required for Light-induced Signaling in the Suprachiasmatic Nucleus Circadian Clock
Section: title/abstract
Claim 42requirement for signalingsupports2003Source 1needs review

CREB-dependent activation of Per1 is required for light-induced signaling in the suprachiasmatic nucleus circadian clock.

Ca2+/cAMP Response Element-binding Protein (CREB)-dependent Activation of Per1 Is Required for Light-induced Signaling in the Suprachiasmatic Nucleus Circadian Clock
Section: title/abstract

Approval Evidence

1 source5 linked approval claimsfirst-pass slug ca2-camp-response-element-decoy-oligodeoxynucleotide
Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.

Source:

gene expression effectsupports

CRE-decoy oligodeoxynucleotide blocks glutamate-induced accumulation of Per1 mRNA.

Furthermore, the CRE-decoy blocked GLU-induced accumulation of Per1 mRNA.

Source:

pathway component rolesupports

CREB and Per1 are integral components of the pathway that transduces light-stimulated glutamatergic neurotransmission into phase advance of the circadian clock.

Thus, Ca(2+)/cAMP response element-binding protein (CREB) and Per1 are integral components of the pathway transducing light-stimulated GLU neurotransmission into phase advance of the circadian clock.

Source:

perturbation effectsupports

CRE-decoy oligodeoxynucleotide blocks glutamate-induced phase advances in the rat suprachiasmatic nucleus.

Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca(2+)/cAMP response element (CRE)-decoy ODN.

Source:

perturbation effectsupports

CRE-decoy oligodeoxynucleotide blocks light-induced phase advances in vivo.

CRE-decoy ODN also blocked light-induced phase advances in vivo.

Source:

requirement for signalingsupports

CREB-dependent activation of Per1 is required for light-induced signaling in the suprachiasmatic nucleus circadian clock.

Ca2+/cAMP Response Element-binding Protein (CREB)-dependent Activation of Per1 Is Required for Light-induced Signaling in the Suprachiasmatic Nucleus Circadian Clock

Source:

Comparisons

Source-backed strengths

The reported strength is pathway-level functional specificity: the CRE-decoy ODN blocked glutamate-induced Per1 mRNA accumulation and blocked glutamate-induced phase advances in the rat SCN. The same study also placed CREB and Per1 as integral components of the pathway transducing light-stimulated glutamatergic neurotransmission into circadian phase advance.

Ranked Citations

  1. 1.
    StructuralSource 1Journal of Biological Chemistry2003Claim 1Claim 2Claim 3

    Extracted from this source document.