Source 1review2021International Journal of Molecular Sciences
Toolkit/caging/uncaging events
caging/uncaging events
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Caging/uncaging events are optogenetic control strategies in which light is used to regulate the functional state of signaling proteins. In the cited review, they are presented as one of two broad routes, alongside light-regulated protein-protein interactions, for steering signal transduction.
Usefulness & Problems
Why this is useful
These strategies are useful because they provide a light-based means to control signaling protein function in a spatiotemporally defined manner, as framed by the review on optogenetic control of signal transduction. The supplied evidence does not specify particular pathways, proteins, or performance metrics.
Problem solved
Caging/uncaging events help solve the problem of externally controlling signaling proteins with light to steer signal transduction. The evidence supports this general problem framing but does not define a specific molecular bottleneck or application context.
Problem links
Need conditional control of signaling activity
DerivedCaging/uncaging events are optogenetic control strategies that use light to regulate the functional state of signaling proteins. In the cited review, they are presented alongside light-regulated protein-protein interactions as a route for steering signal transduction.
Need precise spatiotemporal control with light input
DerivedCaging/uncaging events are optogenetic control strategies that use light to regulate the functional state of signaling proteins. In the cited review, they are presented alongside light-regulated protein-protein interactions as a route for steering signal transduction.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
signalingInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: spectral hardware requirementoperating role: regulatorswitch architecture: multi componentswitch architecture: uncaging
The supplied evidence indicates only that light is the input modality and that the strategy is used to steer signaling protein function. No construct architecture, chromophore requirement, delivery method, or expression-system details are provided.
The evidence is limited to a high-level review statement and does not identify specific caging modules, target proteins, kinetics, dynamic range, or experimental systems. It also does not establish whether particular implementations have been independently replicated or broadly validated.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
Approval Evidence
1 source1 linked approval claimfirst-pass slug caging-uncaging-events
optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
Source:
mechanism summarysupports
The review frames optogenetic control of signaling proteins as being achieved through light-regulated protein-protein interactions or caging/uncaging events.
we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins
Source:
Comparisons
Source-backed strengths
A key strength supported by the source is that caging/uncaging is recognized as a general optogenetic strategy for regulating signaling protein function with light. No quantitative strengths, wavelength ranges, reversibility data, or organism-specific validations are provided in the supplied evidence.
Compared with Cry2
caging/uncaging events and Cry2 address a similar problem space because they share signaling.
Shared frame: same top-level item type; shared target processes: signaling; shared mechanisms: conformational uncaging, conformational_uncaging; same primary input modality: light
Strengths here: may avoid an exogenous cofactor requirement.
Relative tradeoffs: appears more independently replicated.
Compared with engineered focal adhesion kinase two-input gate
caging/uncaging events and engineered focal adhesion kinase two-input gate address a similar problem space because they share signaling.
Shared frame: same top-level item type; shared target processes: signaling; shared mechanisms: conformational uncaging, conformational_uncaging; same primary input modality: light
Compared with iLID/SspB
caging/uncaging events and iLID/SspB address a similar problem space because they share signaling.
Shared frame: same top-level item type; shared target processes: signaling; shared mechanisms: conformational uncaging, conformational_uncaging; same primary input modality: light
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Ranked Citations
- 1.