Source 1review2017Molecular Medicine Reports
Toolkit/catalytically dead Cas9
catalytically dead Cas9
Also known as: dCas9, inactive catalytically dead CRISPR-associated protein 9
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Catalytically dead Cas9 (dCas9) is an inactive CRISPR-associated protein 9 variant used for CRISPR-based modulation of gene expression rather than nuclease-mediated editing. The supplied review evidence describes dCas9-based CRISPR systems as reliable and specific for altering gene expression.
Usefulness & Problems
Why this is useful
dCas9 is useful as a programmable platform for controlling transcription with CRISPR targeting while avoiding catalytic DNA cleavage. The supplied evidence specifically supports its use for reliable and specific alteration of gene expression.
Problem solved
dCas9 helps address the need for specific and reliable control of gene expression using CRISPR machinery without relying on active Cas9 nuclease function. The evidence does not provide further detail on particular target genes, cell types, or application settings.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Techniques
No technique tags yet.
Target processes
editingImplementation Constraints
The available evidence establishes that the tool is an inactive form of Cas9 used in CRISPR-based gene expression control. No additional implementation details, such as guide RNA design, fusion partners, expression systems, or delivery methods, are provided in the supplied evidence.
The evidence is limited to a review-level statement and does not report quantitative performance, organism-specific validation, or direct experimental comparisons. The supplied material also does not describe construct architecture, delivery strategy, off-target behavior, or functional range beyond gene expression alteration.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The review states that the CRISPR system is reliable and specific for altering gene expression when leveraging catalytically dead Cas9.
The CRISPR system has been documented to be very reliable and specific in altering gene expression, via leveraging inactive catalytically dead CRISPR-associated protein 9 (Cas9).
The review states that the CRISPR system is reliable and specific for altering gene expression when leveraging catalytically dead Cas9.
The CRISPR system has been documented to be very reliable and specific in altering gene expression, via leveraging inactive catalytically dead CRISPR-associated protein 9 (Cas9).
The review states that the CRISPR system is reliable and specific for altering gene expression when leveraging catalytically dead Cas9.
The CRISPR system has been documented to be very reliable and specific in altering gene expression, via leveraging inactive catalytically dead CRISPR-associated protein 9 (Cas9).
The review states that the CRISPR system is reliable and specific for altering gene expression when leveraging catalytically dead Cas9.
The CRISPR system has been documented to be very reliable and specific in altering gene expression, via leveraging inactive catalytically dead CRISPR-associated protein 9 (Cas9).
The review states that the CRISPR system is reliable and specific for altering gene expression when leveraging catalytically dead Cas9.
The CRISPR system has been documented to be very reliable and specific in altering gene expression, via leveraging inactive catalytically dead CRISPR-associated protein 9 (Cas9).
The review states that the CRISPR system is reliable and specific for altering gene expression when leveraging catalytically dead Cas9.
The CRISPR system has been documented to be very reliable and specific in altering gene expression, via leveraging inactive catalytically dead CRISPR-associated protein 9 (Cas9).
The review states that the CRISPR system is reliable and specific for altering gene expression when leveraging catalytically dead Cas9.
The CRISPR system has been documented to be very reliable and specific in altering gene expression, via leveraging inactive catalytically dead CRISPR-associated protein 9 (Cas9).
Approval Evidence
1 source1 linked approval claimfirst-pass slug catalytically-dead-cas9
The CRISPR system has been documented to be very reliable and specific in altering gene expression, via leveraging inactive catalytically dead CRISPR-associated protein 9 (Cas9).
Source:
performance summarysupports
The review states that the CRISPR system is reliable and specific for altering gene expression when leveraging catalytically dead Cas9.
The CRISPR system has been documented to be very reliable and specific in altering gene expression, via leveraging inactive catalytically dead CRISPR-associated protein 9 (Cas9).
Source:
Comparisons
Source-backed strengths
The supplied review states that CRISPR systems leveraging inactive catalytically dead Cas9 are very reliable and specific in altering gene expression. This supports dCas9 as a precise gene-expression control tool within the scope of the cited review.
Source:
The CRISPR system has been documented to be very reliable and specific in altering gene expression, via leveraging inactive catalytically dead CRISPR-associated protein 9 (Cas9).
Ranked Citations
- 1.