Source 1review2018Protein Science
Toolkit/chimeric enzymes with new regulatory functions
chimeric enzymes with new regulatory functions
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Protein engineering efforts have been directed towards manipulating enzyme structural dynamics and conformational changes, including targeting specific amino acid interactions and creation of chimeric enzymes with new regulatory functions.
Usefulness & Problems
Why this is useful
This construct pattern combines enzyme engineering with chimeric design to create enzymes that have new regulatory functions. In the review framing, it is used to manipulate structural dynamics and conformational change.; engineering new regulatory control into enzymes; rewiring enzyme conformational behavior
Source:
This construct pattern combines enzyme engineering with chimeric design to create enzymes that have new regulatory functions. In the review framing, it is used to manipulate structural dynamics and conformational change.
Source:
engineering new regulatory control into enzymes
Source:
rewiring enzyme conformational behavior
Problem solved
It addresses the problem of adding controllable regulation to enzymes by redesigning how conformational changes are coupled to function.; introducing new regulatory functions into enzymes
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It addresses the problem of adding controllable regulation to enzymes by redesigning how conformational changes are coupled to function.
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introducing new regulatory functions into enzymes
Problem links
introducing new regulatory functions into enzymes
LiteratureIt addresses the problem of adding controllable regulation to enzymes by redesigning how conformational changes are coupled to function.
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It addresses the problem of adding controllable regulation to enzymes by redesigning how conformational changes are coupled to function.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
Structural CharacterizationTarget processes
recombinationImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: regulatorswitch architecture: uncaging
It requires engineered enzyme constructs and protein-design or molecular-biology workflows capable of building chimeric proteins. The abstract does not specify particular domains, hosts, or assays.; requires protein engineering of chimeric enzyme constructs
The abstract does not show that chimeric design alone solves all issues of catalytic efficiency, expression, or generalizability across enzymes.; specific architectures and performance tradeoffs are not described in the abstract
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The ability to engineer and control enzyme structural dynamics can provide new tools for biotechnology and medicine.
Post-translational covalent modification can provide an additional level of enzyme control.
Protein engineering efforts have manipulated enzyme structural dynamics and conformational changes by targeting specific amino acid interactions and by creating chimeric enzymes with new regulatory functions.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug chimeric-enzymes-with-new-regulatory-functions
Protein engineering efforts have been directed towards manipulating enzyme structural dynamics and conformational changes, including targeting specific amino acid interactions and creation of chimeric enzymes with new regulatory functions.
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application potentialsupports
The ability to engineer and control enzyme structural dynamics can provide new tools for biotechnology and medicine.
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engineering strategy summarysupports
Protein engineering efforts have manipulated enzyme structural dynamics and conformational changes by targeting specific amino acid interactions and by creating chimeric enzymes with new regulatory functions.
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Comparisons
Source-stated alternatives
The abstract contrasts chimeric design with targeting specific amino acid interactions and with post-translational covalent modification as other control strategies.
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The abstract contrasts chimeric design with targeting specific amino acid interactions and with post-translational covalent modification as other control strategies.
Source-backed strengths
supports creation of new regulatory functions
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supports creation of new regulatory functions
chimeric enzymes with new regulatory functions and CheRiff + jRCaMP1b + RH237 cardiac all-optical electrophysiology platform address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination
Strengths here: looks easier to implement in practice.
Compared with joining proteins in creative ways
chimeric enzymes with new regulatory functions and joining proteins in creative ways address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; shared mechanisms: conformational_uncaging
chimeric enzymes with new regulatory functions and tetraphenylethylene self-assembled monolayer mechano-optoelectronic molecular switch address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; shared mechanisms: conformational_uncaging
Ranked Citations
- 1.