Toolkit/chromatin in vivo imaging

chromatin in vivo imaging

Assay Method·Research·Since 2018

Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.

Summary

Chromatin in vivo imaging is identified in a 2018 review as a CRISPR/Cas9-based epigenetic technique for imaging chromatin in living systems. The supplied evidence supports its existence as a method category within the CRISPR/Cas9 epigenetics toolkit, but does not describe a specific construct, protocol, or performance profile.

Usefulness & Problems

Why this is useful

This method category is useful because it extends CRISPR/Cas9 technology into epigenetics and enables chromatin-focused investigation in vivo. The available evidence only establishes that chromatin in vivo imaging is considered one of the novel CRISPR/Cas9-based epigenetic techniques discussed in the review.

Problem solved

It addresses the general need for visualizing chromatin in living biological contexts using CRISPR/Cas9-based approaches. The supplied evidence does not specify which chromatin features, loci, or dynamic processes were measured.

Problem links

Need better screening or enrichment leverage

Derived

Chromatin in vivo imaging is identified in the cited review as a CRISPR/Cas9-based epigenetic technique. The supplied evidence establishes its inclusion as a method category for visualizing chromatin in living systems, but does not provide implementation or performance details.

Need conditional recombination or state switching

Derived

Chromatin in vivo imaging is identified in the cited review as a CRISPR/Cas9-based epigenetic technique. The supplied evidence establishes its inclusion as a method category for visualizing chromatin in living systems, but does not provide implementation or performance details.

Need controllable genome or transcript editing

Derived

Chromatin in vivo imaging is identified in the cited review as a CRISPR/Cas9-based epigenetic technique. The supplied evidence establishes its inclusion as a method category for visualizing chromatin in living systems, but does not provide implementation or performance details.

Taxonomy & Function

Primary hierarchy

Technique Branch

Method: A concrete measurement method used to characterize an engineered system.

Mechanisms

No mechanism tags yet.

Target processes

editingrecombinationselection

Implementation Constraints

cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor

The only implementation-related fact supported by the evidence is that the method is CRISPR/Cas9-based. No details are given regarding Cas9 variant, guide RNA design, fluorescent labeling strategy, expression system, or imaging instrumentation.

The evidence is limited to review-level mention and does not provide implementation details, experimental data, or comparative benchmarks. No information is supplied on target specificity, imaging modality, organism, delivery strategy, or reproducibility.

Validation

Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication

Supporting Sources

Ranked Claims

Claim 1field impactsupports2018Source 1needs review

The emergence of CRISPR/Cas9 technology has provided new routes into the epigenetics field.

In recent years, the emergence of CRISPR/Cas9 technology has provided us with new routes to the epigenetic field.
Claim 2field impactsupports2018Source 1needs review

The emergence of CRISPR/Cas9 technology has provided new routes into the epigenetics field.

In recent years, the emergence of CRISPR/Cas9 technology has provided us with new routes to the epigenetic field.
Claim 3field impactsupports2018Source 1needs review

The emergence of CRISPR/Cas9 technology has provided new routes into the epigenetics field.

In recent years, the emergence of CRISPR/Cas9 technology has provided us with new routes to the epigenetic field.
Claim 4field impactsupports2018Source 1needs review

The emergence of CRISPR/Cas9 technology has provided new routes into the epigenetics field.

In recent years, the emergence of CRISPR/Cas9 technology has provided us with new routes to the epigenetic field.
Claim 5field impactsupports2018Source 1needs review

The emergence of CRISPR/Cas9 technology has provided new routes into the epigenetics field.

In recent years, the emergence of CRISPR/Cas9 technology has provided us with new routes to the epigenetic field.
Claim 6field impactsupports2018Source 1needs review

The emergence of CRISPR/Cas9 technology has provided new routes into the epigenetics field.

In recent years, the emergence of CRISPR/Cas9 technology has provided us with new routes to the epigenetic field.
Claim 7field impactsupports2018Source 1needs review

The emergence of CRISPR/Cas9 technology has provided new routes into the epigenetics field.

In recent years, the emergence of CRISPR/Cas9 technology has provided us with new routes to the epigenetic field.
Claim 8field impactsupports2018Source 1needs review

The emergence of CRISPR/Cas9 technology has provided new routes into the epigenetics field.

In recent years, the emergence of CRISPR/Cas9 technology has provided us with new routes to the epigenetic field.
Claim 9field impactsupports2018Source 1needs review

The emergence of CRISPR/Cas9 technology has provided new routes into the epigenetics field.

In recent years, the emergence of CRISPR/Cas9 technology has provided us with new routes to the epigenetic field.
Claim 10field impactsupports2018Source 1needs review

The emergence of CRISPR/Cas9 technology has provided new routes into the epigenetics field.

In recent years, the emergence of CRISPR/Cas9 technology has provided us with new routes to the epigenetic field.
Claim 11review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 12review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 13review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 14review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 15review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 16review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 17review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 18review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 19review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 20review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 21review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 22review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 23review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 24review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 25review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 26review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 27review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 28review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 29review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 30review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 31review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 32review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 33review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 34review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 35review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 36review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
Claim 37review scopesupports2018Source 1needs review

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

Approval Evidence

1 source1 linked approval claimfirst-pass slug chromatin-in-vivo-imaging
In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including ... chromatin in vivo imaging...

Source:

review scopesupports

The review covers CRISPR/Cas9-based epigenetic techniques including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.

Source:

Comparisons

Source-backed strengths

A documented strength is its placement within the broader emergence of CRISPR/Cas9 technologies that opened new routes into the epigenetics field. No direct evidence is provided for sensitivity, resolution, targeting range, temporal performance, or validation outcomes.

chromatin in vivo imaging and epigenetic element screening address a similar problem space because they share editing, recombination, selection.

Shared frame: same top-level item type; shared target processes: editing, recombination, selection

chromatin in vivo imaging and high throughput screening address a similar problem space because they share editing, recombination, selection.

Shared frame: same top-level item type; shared target processes: editing, recombination, selection

chromatin in vivo imaging and whole genome screening of gene knockout mutants address a similar problem space because they share editing, recombination, selection.

Shared frame: same top-level item type; shared target processes: editing, recombination, selection

Ranked Citations

  1. 1.
    StructuralSource 1Stem Cells International2018Claim 10Claim 10Claim 10

    Seeded from load plan for claim cl1. Extracted from this source document.