Source 1primary paper2021Nature Communications
Toolkit/click-labelling
click-labelling
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Click-labelling in this context is a Bacillus subtilis genetic code expansion platform that incorporates noncanonical amino acids for click-chemistry-based protein labelling. In the cited 2021 study, it was implemented within broad and efficient stop-codon suppression systems and used alongside photo-crosslinking and translational titration applications.
Usefulness & Problems
Why this is useful
This approach enables site-specific installation of click-reactive noncanonical amino acids in B. subtilis proteins, providing a route to chemical labelling in a bacterial chassis. The same platform also supports translational control applications, increasing its utility for probing protein function and cellular processes in vivo.
Source:
We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
Source:
we demonstrate broad and efficient genetic code expansion in B. subtilis by incorporating 20 distinct non-standard amino acids within proteins using 3 different families of genetic code expansion systems and two choices of codons
Problem solved
It addresses the need for efficient genetic code expansion in Bacillus subtilis to support site-specific protein labelling and related translational manipulation. The study specifically positions the platform as a way to interrogate bacterial cytokinesis by precisely modulating cell division dynamics in vivo.
Source:
We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
Problem links
This item provides a directly usable genetic-code-expansion implementation for introducing noncanonical amino acids into proteins, which could support more programmable protein polymer chemistry. It is weaker than CFPS because the supplied evidence is focused on labeling and stop-codon suppression rather than general polymer synthesis.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
translationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: builder
The implementation is based on genetic code expansion and stop-codon suppression in Bacillus subtilis. The evidence states that three families of genetic code expansion systems and two codon choices were used, but it does not provide construct architecture, orthogonal synthetase/tRNA identities, or reagent requirements for the click-labelling workflow.
The supplied evidence does not specify the noncanonical amino acids, click reaction chemistry, labeling performance metrics, or target proteins used for click-labelling. Independent replication is not provided in the evidence, and validation appears limited to the reported study in Bacillus subtilis.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successBacteriaapplication demoBacillus subtilis
Inferred from claim c2 during normalization. The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration. Derived from claim c2. Quoted text: We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
Source:
successBacteriaapplication demoBacillus subtilis
Inferred from claim c2 during normalization. The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration. Derived from claim c2. Quoted text: We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
Source:
successBacteriaapplication demoBacillus subtilis
Inferred from claim c2 during normalization. The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration. Derived from claim c2. Quoted text: We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
Source:
successBacteriaapplication demoBacillus subtilis
Inferred from claim c2 during normalization. The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration. Derived from claim c2. Quoted text: We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
Source:
successBacteriaapplication demoBacillus subtilis
Inferred from claim c2 during normalization. The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration. Derived from claim c2. Quoted text: We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
Source:
successBacteriaapplication demoBacillus subtilis
Inferred from claim c2 during normalization. The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration. Derived from claim c2. Quoted text: We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
Source:
successBacteriaapplication demoBacillus subtilis
Inferred from claim c2 during normalization. The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration. Derived from claim c2. Quoted text: We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
Source:
Supporting Sources
Ranked Claims
The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration.
We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration.
We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration.
We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration.
We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration.
We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration.
We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration.
We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
These tools were used to begin interrogating properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo.
begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo
These tools were used to begin interrogating properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo.
begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo
These tools were used to begin interrogating properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo.
begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo
These tools were used to begin interrogating properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo.
begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo
These tools were used to begin interrogating properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo.
begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo
These tools were used to begin interrogating properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo.
begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo
These tools were used to begin interrogating properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo.
begin to interrogate properties underlying bacterial cytokinesis by precisely modulating cell division dynamics in vivo
The authors demonstrate broad and efficient genetic code expansion in Bacillus subtilis using 3 families of genetic code expansion systems and 2 codon choices.
we demonstrate broad and efficient genetic code expansion in B. subtilis by incorporating 20 distinct non-standard amino acids within proteins using 3 different families of genetic code expansion systems and two choices of codons
codon choices 2distinct non-standard amino acids incorporated 20genetic code expansion system families 3
These tools allowed the authors to demonstrate differences between E. coli and Bacillus subtilis stop codon suppression.
These tools allow us to demonstrate differences between E. coli and B. subtilis stop codon suppression
The paper concerns designing efficient genetic code expansion in Bacillus subtilis to gain biological insights.
Designing efficient genetic code expansion in Bacillus subtilis to gain biological insights
Section: title
These tools were used to validate a predicted protein-protein binding interface.
validate a predicted protein-protein binding interface
These tools were used to validate a predicted protein-protein binding interface.
validate a predicted protein-protein binding interface
These tools were used to validate a predicted protein-protein binding interface.
validate a predicted protein-protein binding interface
These tools were used to validate a predicted protein-protein binding interface.
validate a predicted protein-protein binding interface
These tools were used to validate a predicted protein-protein binding interface.
validate a predicted protein-protein binding interface
These tools were used to validate a predicted protein-protein binding interface.
validate a predicted protein-protein binding interface
These tools were used to validate a predicted protein-protein binding interface.
validate a predicted protein-protein binding interface
Approval Evidence
1 source1 linked approval claimfirst-pass slug click-labelling
We use these systems to achieve click-labelling
Source:
applicationsupports
The genetic code expansion systems were used to achieve click-labelling, photo-crosslinking, and translational titration.
We use these systems to achieve click-labelling, photo-crosslinking, and translational titration.
Source:
Comparisons
Source-backed strengths
The source reports broad and efficient genetic code expansion in B. subtilis using three families of systems and two codon choices. The platform was demonstrated for multiple use cases, including click-labelling, photo-crosslinking, and translational titration, indicating functional versatility within the same host organism.
Source:
These tools allow us to demonstrate differences between E. coli and B. subtilis stop codon suppression
Compared with brain stimulation
click-labelling and brain stimulation address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control; same primary input modality: light
Compared with genetic code expansion in Bacillus subtilis
click-labelling and genetic code expansion in Bacillus subtilis address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation control, translation_control; same primary input modality: light
Relative tradeoffs: looks easier to implement in practice.
Compared with thermal sonogenetics
click-labelling and thermal sonogenetics address a similar problem space because they share translation.
Shared frame: same top-level item type; shared target processes: translation; shared mechanisms: translation_control; same primary input modality: light
Ranked Citations
- 1.