Source 1review2019PLANT PHYSIOLOGY
Toolkit/closed-loop molecular and cellular circuits
closed-loop molecular and cellular circuits
Also known as: semi- and fully synthetic closed-loop circuits
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Closed-loop molecular and cellular circuits are semi- and fully synthetic regulatory circuit architectures described in plants. They are assembled from genetic parts to build open- and closed-loop control systems intended to regulate biological processes such as signaling and recombination.
Usefulness & Problems
Why this is useful
These circuits are presented as plant synthetic biology tools for probing complex signaling networks and for engineering traits relevant to crop productivity and biopharmaceutical production. Their usefulness derives from the ability to organize genetic components into regulatory architectures of increasing complexity.
Source:
we explore potential applications of these approaches for the engineering of novel functionalities in plants, including understanding complex signaling networks, improving crop productivity, and the production of biopharmaceuticals
Problem solved
They address the problem of constructing synthetic regulatory systems in plants that can control biological processes through defined circuit architectures. The cited literature specifically frames these systems as a way to understand and manipulate complex signaling networks.
Source:
we explore potential applications of these approaches for the engineering of novel functionalities in plants, including understanding complex signaling networks, improving crop productivity, and the production of biopharmaceuticals
Problem links
Need conditional control of signaling activity
DerivedClosed-loop molecular and cellular circuits are semi- and fully synthetic regulatory circuit architectures described in plants. They are assembled from genetic parts to build open- and closed-loop control systems intended to regulate biological processes such as signaling and recombination.
Need conditional recombination or state switching
DerivedClosed-loop molecular and cellular circuits are semi- and fully synthetic regulatory circuit architectures described in plants. They are assembled from genetic parts to build open- and closed-loop control systems intended to regulate biological processes such as signaling and recombination.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
regulatory circuit feedbackTechniques
No technique tags yet.
Target processes
recombinationsignalingImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: regulator
Implementation is described at the level of modular assembly of genetic parts for building synthetic circuits in plants. The evidence does not specify promoters, host species, delivery methods, cofactors, or construct topologies beyond the existence of open- and closed-loop architectures.
The supplied evidence does not provide specific performance data, quantitative benchmarks, or detailed examples of closed-loop circuit behavior in plants. It also does not document independent replication or compare semi-synthetic versus fully synthetic implementations.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Synthetic switches and regulatory circuits in plants are discussed as enabling understanding of complex signaling networks, improving crop productivity, and producing biopharmaceuticals.
we explore potential applications of these approaches for the engineering of novel functionalities in plants, including understanding complex signaling networks, improving crop productivity, and the production of biopharmaceuticals
Synthetic switches and regulatory circuits in plants are discussed as enabling understanding of complex signaling networks, improving crop productivity, and producing biopharmaceuticals.
we explore potential applications of these approaches for the engineering of novel functionalities in plants, including understanding complex signaling networks, improving crop productivity, and the production of biopharmaceuticals
Synthetic switches and regulatory circuits in plants are discussed as enabling understanding of complex signaling networks, improving crop productivity, and producing biopharmaceuticals.
we explore potential applications of these approaches for the engineering of novel functionalities in plants, including understanding complex signaling networks, improving crop productivity, and the production of biopharmaceuticals
Synthetic switches and regulatory circuits in plants are discussed as enabling understanding of complex signaling networks, improving crop productivity, and producing biopharmaceuticals.
we explore potential applications of these approaches for the engineering of novel functionalities in plants, including understanding complex signaling networks, improving crop productivity, and the production of biopharmaceuticals
Synthetic switches and regulatory circuits in plants are discussed as enabling understanding of complex signaling networks, improving crop productivity, and producing biopharmaceuticals.
we explore potential applications of these approaches for the engineering of novel functionalities in plants, including understanding complex signaling networks, improving crop productivity, and the production of biopharmaceuticals
Synthetic switches and regulatory circuits in plants are discussed as enabling understanding of complex signaling networks, improving crop productivity, and producing biopharmaceuticals.
we explore potential applications of these approaches for the engineering of novel functionalities in plants, including understanding complex signaling networks, improving crop productivity, and the production of biopharmaceuticals
Synthetic switches and regulatory circuits in plants are discussed as enabling understanding of complex signaling networks, improving crop productivity, and producing biopharmaceuticals.
we explore potential applications of these approaches for the engineering of novel functionalities in plants, including understanding complex signaling networks, improving crop productivity, and the production of biopharmaceuticals
Modular assembly of genetic parts is presented as a strategy for building synthetic circuits of increasing complexity.
We highlight the strategies for the modular assembly of genetic parts into synthetic circuits of different complexity, ranging from Boolean logic gates and oscillatory devices up to semi- and fully synthetic open- and closed-loop molecular and cellular circuits
Modular assembly of genetic parts is presented as a strategy for building synthetic circuits of increasing complexity.
We highlight the strategies for the modular assembly of genetic parts into synthetic circuits of different complexity, ranging from Boolean logic gates and oscillatory devices up to semi- and fully synthetic open- and closed-loop molecular and cellular circuits
Modular assembly of genetic parts is presented as a strategy for building synthetic circuits of increasing complexity.
We highlight the strategies for the modular assembly of genetic parts into synthetic circuits of different complexity, ranging from Boolean logic gates and oscillatory devices up to semi- and fully synthetic open- and closed-loop molecular and cellular circuits
Modular assembly of genetic parts is presented as a strategy for building synthetic circuits of increasing complexity.
We highlight the strategies for the modular assembly of genetic parts into synthetic circuits of different complexity, ranging from Boolean logic gates and oscillatory devices up to semi- and fully synthetic open- and closed-loop molecular and cellular circuits
Modular assembly of genetic parts is presented as a strategy for building synthetic circuits of increasing complexity.
We highlight the strategies for the modular assembly of genetic parts into synthetic circuits of different complexity, ranging from Boolean logic gates and oscillatory devices up to semi- and fully synthetic open- and closed-loop molecular and cellular circuits
Modular assembly of genetic parts is presented as a strategy for building synthetic circuits of increasing complexity.
We highlight the strategies for the modular assembly of genetic parts into synthetic circuits of different complexity, ranging from Boolean logic gates and oscillatory devices up to semi- and fully synthetic open- and closed-loop molecular and cellular circuits
Modular assembly of genetic parts is presented as a strategy for building synthetic circuits of increasing complexity.
We highlight the strategies for the modular assembly of genetic parts into synthetic circuits of different complexity, ranging from Boolean logic gates and oscillatory devices up to semi- and fully synthetic open- and closed-loop molecular and cellular circuits
Approval Evidence
1 source2 linked approval claimsfirst-pass slug closed-loop-molecular-and-cellular-circuits
up to semi- and fully synthetic open- and closed-loop molecular and cellular circuits
Source:
application potentialsupports
Synthetic switches and regulatory circuits in plants are discussed as enabling understanding of complex signaling networks, improving crop productivity, and producing biopharmaceuticals.
we explore potential applications of these approaches for the engineering of novel functionalities in plants, including understanding complex signaling networks, improving crop productivity, and the production of biopharmaceuticals
Source:
design strategysupports
Modular assembly of genetic parts is presented as a strategy for building synthetic circuits of increasing complexity.
We highlight the strategies for the modular assembly of genetic parts into synthetic circuits of different complexity, ranging from Boolean logic gates and oscillatory devices up to semi- and fully synthetic open- and closed-loop molecular and cellular circuits
Source:
Comparisons
Source-backed strengths
A stated strength is the modular assembly of genetic parts, which supports construction of synthetic circuits with increasing complexity. The literature also positions plant synthetic switches and regulatory circuits as having application potential in crop improvement and biopharmaceutical production.
Compared with CAR-NK
closed-loop molecular and cellular circuits and CAR-NK address a similar problem space because they share recombination, signaling.
Shared frame: same top-level item type; shared target processes: recombination, signaling
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Compared with chimeric trace-amine-associated receptor 1
closed-loop molecular and cellular circuits and chimeric trace-amine-associated receptor 1 address a similar problem space because they share recombination, signaling.
Shared frame: same top-level item type; shared target processes: recombination, signaling
closed-loop molecular and cellular circuits and H2O2-responsive promoter-driven nuclear-encoded reporter gene address a similar problem space because they share recombination, signaling.
Shared frame: same top-level item type; shared target processes: recombination, signaling
Strengths here: looks easier to implement in practice.
Ranked Citations
- 1.