Source 1primary paper2022
Toolkit/CluMPS
CluMPS
Also known as: Clusters Magnified by Phase Separation
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
CluMPS (Clusters Magnified by Phase Separation) is a fluorescent reporter strategy for high-sensitivity detection of protein clusters in cells. It is reported to visualize submicroscopic clusters, including small aggregates, and to track clusters of unmodified, tagged, and endogenous proteins.
Usefulness & Problems
Why this is useful
CluMPS is useful for visualizing protein clustering events that are difficult to resolve with conventional diffuse fluorescent protein fusions. The reported ability to detect small pathological protein aggregates and to support orthogonal multiplexed probes indicates utility for sensitive cellular imaging of multiple clustering targets.
Source:
CluMPS also detected and tracked clusters of unmodified and tagged endogenous proteins, and orthogonal CluMPS probes could be multiplexed in cells.
Source:
Here we describe a fluorescent reporter strategy that detects protein clusters with high sensitivity, called CluMPS
Problem solved
CluMPS addresses the problem of detecting submicroscopic protein clusters in cells when standard imaging approaches, including corresponding GFP fusions, can appear diffuse. It is specifically positioned to reveal small oligomeric or aggregated states that are otherwise hard to visualize.
Source:
CluMPS also detected and tracked clusters of unmodified and tagged endogenous proteins, and orthogonal CluMPS probes could be multiplexed in cells.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The available evidence supports that CluMPS functions as a fluorescent reporter in cells and has been applied to unmodified, tagged, and endogenous proteins. However, the supplied material does not define fluorophores, construct design, delivery method, cofactors, or whether any light-dependent activation step is required.
The provided evidence does not specify quantitative performance metrics, dynamic range, false-positive behavior, or the full range of validated target proteins. Mechanistic and implementation details are limited in the supplied material, so construct architecture, required binding modules, and imaging constraints cannot be fully assessed here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
CluMPS detected and tracked clusters of unmodified and tagged endogenous proteins, and orthogonal CluMPS probes could be multiplexed in cells.
CluMPS also detected and tracked clusters of unmodified and tagged endogenous proteins, and orthogonal CluMPS probes could be multiplexed in cells.
CluMPS detected and tracked clusters of unmodified and tagged endogenous proteins, and orthogonal CluMPS probes could be multiplexed in cells.
CluMPS also detected and tracked clusters of unmodified and tagged endogenous proteins, and orthogonal CluMPS probes could be multiplexed in cells.
CluMPS detected and tracked clusters of unmodified and tagged endogenous proteins, and orthogonal CluMPS probes could be multiplexed in cells.
CluMPS also detected and tracked clusters of unmodified and tagged endogenous proteins, and orthogonal CluMPS probes could be multiplexed in cells.
CluMPS detected and tracked clusters of unmodified and tagged endogenous proteins, and orthogonal CluMPS probes could be multiplexed in cells.
CluMPS also detected and tracked clusters of unmodified and tagged endogenous proteins, and orthogonal CluMPS probes could be multiplexed in cells.
CluMPS detected and tracked clusters of unmodified and tagged endogenous proteins, and orthogonal CluMPS probes could be multiplexed in cells.
CluMPS also detected and tracked clusters of unmodified and tagged endogenous proteins, and orthogonal CluMPS probes could be multiplexed in cells.
CluMPS detected and tracked clusters of unmodified and tagged endogenous proteins, and orthogonal CluMPS probes could be multiplexed in cells.
CluMPS also detected and tracked clusters of unmodified and tagged endogenous proteins, and orthogonal CluMPS probes could be multiplexed in cells.
CluMPS detected and tracked clusters of unmodified and tagged endogenous proteins, and orthogonal CluMPS probes could be multiplexed in cells.
CluMPS also detected and tracked clusters of unmodified and tagged endogenous proteins, and orthogonal CluMPS probes could be multiplexed in cells.
CluMPS is a fluorescent reporter strategy that detects protein clusters with high sensitivity.
Here we describe a fluorescent reporter strategy that detects protein clusters with high sensitivity, called CluMPS
CluMPS is a fluorescent reporter strategy that detects protein clusters with high sensitivity.
Here we describe a fluorescent reporter strategy that detects protein clusters with high sensitivity, called CluMPS
CluMPS is a fluorescent reporter strategy that detects protein clusters with high sensitivity.
Here we describe a fluorescent reporter strategy that detects protein clusters with high sensitivity, called CluMPS
CluMPS is a fluorescent reporter strategy that detects protein clusters with high sensitivity.
Here we describe a fluorescent reporter strategy that detects protein clusters with high sensitivity, called CluMPS
CluMPS is a fluorescent reporter strategy that detects protein clusters with high sensitivity.
Here we describe a fluorescent reporter strategy that detects protein clusters with high sensitivity, called CluMPS
CluMPS is a fluorescent reporter strategy that detects protein clusters with high sensitivity.
Here we describe a fluorescent reporter strategy that detects protein clusters with high sensitivity, called CluMPS
CluMPS is a fluorescent reporter strategy that detects protein clusters with high sensitivity.
Here we describe a fluorescent reporter strategy that detects protein clusters with high sensitivity, called CluMPS
CluMPS detected small aggregates of pathological proteins in cases where corresponding GFP fusions appeared diffuse.
CluMPS detected small aggregates of pathological proteins where the corresponding GFP fusions appeared diffuse.
CluMPS detected small aggregates of pathological proteins in cases where corresponding GFP fusions appeared diffuse.
CluMPS detected small aggregates of pathological proteins where the corresponding GFP fusions appeared diffuse.
CluMPS detected small aggregates of pathological proteins in cases where corresponding GFP fusions appeared diffuse.
CluMPS detected small aggregates of pathological proteins where the corresponding GFP fusions appeared diffuse.
CluMPS detected small aggregates of pathological proteins in cases where corresponding GFP fusions appeared diffuse.
CluMPS detected small aggregates of pathological proteins where the corresponding GFP fusions appeared diffuse.
CluMPS detected small aggregates of pathological proteins in cases where corresponding GFP fusions appeared diffuse.
CluMPS detected small aggregates of pathological proteins where the corresponding GFP fusions appeared diffuse.
CluMPS detected small aggregates of pathological proteins in cases where corresponding GFP fusions appeared diffuse.
CluMPS detected small aggregates of pathological proteins where the corresponding GFP fusions appeared diffuse.
CluMPS detected small aggregates of pathological proteins in cases where corresponding GFP fusions appeared diffuse.
CluMPS detected small aggregates of pathological proteins where the corresponding GFP fusions appeared diffuse.
CluMPS can detect small oligomers and behaves rationally according to key system parameters.
We use computational modeling and optogenetic clustering to demonstrate that CluMPS can detect small oligomers and behaves rationally according to key system parameters.
CluMPS can detect small oligomers and behaves rationally according to key system parameters.
We use computational modeling and optogenetic clustering to demonstrate that CluMPS can detect small oligomers and behaves rationally according to key system parameters.
CluMPS can detect small oligomers and behaves rationally according to key system parameters.
We use computational modeling and optogenetic clustering to demonstrate that CluMPS can detect small oligomers and behaves rationally according to key system parameters.
CluMPS can detect small oligomers and behaves rationally according to key system parameters.
We use computational modeling and optogenetic clustering to demonstrate that CluMPS can detect small oligomers and behaves rationally according to key system parameters.
CluMPS can detect small oligomers and behaves rationally according to key system parameters.
We use computational modeling and optogenetic clustering to demonstrate that CluMPS can detect small oligomers and behaves rationally according to key system parameters.
CluMPS can detect small oligomers and behaves rationally according to key system parameters.
We use computational modeling and optogenetic clustering to demonstrate that CluMPS can detect small oligomers and behaves rationally according to key system parameters.
CluMPS can detect small oligomers and behaves rationally according to key system parameters.
We use computational modeling and optogenetic clustering to demonstrate that CluMPS can detect small oligomers and behaves rationally according to key system parameters.
A CluMPS reporter visually amplifies small clusters of a binding partner by generating large, quantifiable fluorescence condensates.
A CluMPS reporter detects and visually amplifies even small clusters of a binding partner, generating large, quantifiable fluorescence condensates.
A CluMPS reporter visually amplifies small clusters of a binding partner by generating large, quantifiable fluorescence condensates.
A CluMPS reporter detects and visually amplifies even small clusters of a binding partner, generating large, quantifiable fluorescence condensates.
A CluMPS reporter visually amplifies small clusters of a binding partner by generating large, quantifiable fluorescence condensates.
A CluMPS reporter detects and visually amplifies even small clusters of a binding partner, generating large, quantifiable fluorescence condensates.
A CluMPS reporter visually amplifies small clusters of a binding partner by generating large, quantifiable fluorescence condensates.
A CluMPS reporter detects and visually amplifies even small clusters of a binding partner, generating large, quantifiable fluorescence condensates.
A CluMPS reporter visually amplifies small clusters of a binding partner by generating large, quantifiable fluorescence condensates.
A CluMPS reporter detects and visually amplifies even small clusters of a binding partner, generating large, quantifiable fluorescence condensates.
A CluMPS reporter visually amplifies small clusters of a binding partner by generating large, quantifiable fluorescence condensates.
A CluMPS reporter detects and visually amplifies even small clusters of a binding partner, generating large, quantifiable fluorescence condensates.
A CluMPS reporter visually amplifies small clusters of a binding partner by generating large, quantifiable fluorescence condensates.
A CluMPS reporter detects and visually amplifies even small clusters of a binding partner, generating large, quantifiable fluorescence condensates.
CluMPS provides an approach to observe higher-order protein assembly in native cellular context.
CluMPS provides a powerful yet straightforward approach to observe higher-order protein assembly in its native cellular context.
CluMPS provides an approach to observe higher-order protein assembly in native cellular context.
CluMPS provides a powerful yet straightforward approach to observe higher-order protein assembly in its native cellular context.
CluMPS provides an approach to observe higher-order protein assembly in native cellular context.
CluMPS provides a powerful yet straightforward approach to observe higher-order protein assembly in its native cellular context.
CluMPS provides an approach to observe higher-order protein assembly in native cellular context.
CluMPS provides a powerful yet straightforward approach to observe higher-order protein assembly in its native cellular context.
CluMPS provides an approach to observe higher-order protein assembly in native cellular context.
CluMPS provides a powerful yet straightforward approach to observe higher-order protein assembly in its native cellular context.
CluMPS provides an approach to observe higher-order protein assembly in native cellular context.
CluMPS provides a powerful yet straightforward approach to observe higher-order protein assembly in its native cellular context.
CluMPS provides an approach to observe higher-order protein assembly in native cellular context.
CluMPS provides a powerful yet straightforward approach to observe higher-order protein assembly in its native cellular context.
Approval Evidence
1 source6 linked approval claimsfirst-pass slug clumps
Here we describe a fluorescent reporter strategy that detects protein clusters with high sensitivity, called CluMPS ( Clu sters M agnified by P hase S eparation).
Source:
application scopesupports
CluMPS detected and tracked clusters of unmodified and tagged endogenous proteins, and orthogonal CluMPS probes could be multiplexed in cells.
CluMPS also detected and tracked clusters of unmodified and tagged endogenous proteins, and orthogonal CluMPS probes could be multiplexed in cells.
Source:
capabilitysupports
CluMPS is a fluorescent reporter strategy that detects protein clusters with high sensitivity.
Here we describe a fluorescent reporter strategy that detects protein clusters with high sensitivity, called CluMPS
Source:
comparative detectionsupports
CluMPS detected small aggregates of pathological proteins in cases where corresponding GFP fusions appeared diffuse.
CluMPS detected small aggregates of pathological proteins where the corresponding GFP fusions appeared diffuse.
Source:
detection performancesupports
CluMPS can detect small oligomers and behaves rationally according to key system parameters.
We use computational modeling and optogenetic clustering to demonstrate that CluMPS can detect small oligomers and behaves rationally according to key system parameters.
Source:
mechanism of actionsupports
A CluMPS reporter visually amplifies small clusters of a binding partner by generating large, quantifiable fluorescence condensates.
A CluMPS reporter detects and visually amplifies even small clusters of a binding partner, generating large, quantifiable fluorescence condensates.
Source:
use contextsupports
CluMPS provides an approach to observe higher-order protein assembly in native cellular context.
CluMPS provides a powerful yet straightforward approach to observe higher-order protein assembly in its native cellular context.
Source:
Comparisons
Source-backed strengths
The source literature reports high-sensitivity detection of protein clusters and successful detection and tracking of clusters from unmodified and tagged endogenous proteins. It also reports that orthogonal CluMPS probes could be multiplexed in cells and that small pathological aggregates were detected in cases where corresponding GFP fusions appeared diffuse.
Source:
CluMPS detected small aggregates of pathological proteins where the corresponding GFP fusions appeared diffuse.
Ranked Citations
- 1.