Source 1primary paper2021
Toolkit/compact PE
compact PE
Also known as: compact PE2, truncated reverse transcriptase prime editor
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Compact PE is a truncated prime editor in which the reverse transcriptase RNase H domain is completely deleted. In the cited 2021 study, this compact PE showed editing activity comparable to full-length PE2 and enabled flexible split prime editor designs for in vivo delivery.
Usefulness & Problems
Why this is useful
This tool is useful because it reduces prime editor size while preserving editing performance in the reported study. The same study positions this size reduction as enabling more flexible split prime editor architectures for efficient in vivo delivery and improving safety by abolishing eRF1 binding with minimal effect on stop codon readthrough.
Source:
This study identifies a safe and efficient compact PE2 that enables flexible split-PE design to facilitate efficient delivery in vivo
Problem solved
Compact PE addresses the engineering problem that full-length prime editors are large and less amenable to flexible split designs for in vivo delivery. It also addresses a safety-related concern by removing the reverse transcriptase RNase H domain associated in the study with eRF1 binding and stop codon readthrough effects.
Source:
This study identifies a safe and efficient compact PE2 that enables flexible split-PE design to facilitate efficient delivery in vivo
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
Computational DesignTarget processes
editingImplementation Constraints
The defining construct feature is complete deletion of the reverse transcriptase RNase H domain from PE2. The study indicates compatibility with split prime editor design for in vivo delivery, but the supplied evidence does not provide construct junctions, split sites, expression systems, or vector specifications.
The evidence provided comes from a single 2021 source and does not specify editing contexts, target range, cell types, or quantitative performance metrics in this summary. No independent replication, delivery vehicle details, or long-term in vivo validation are described in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The study identifies a safe and efficient compact PE2 that enables flexible split prime editor design to facilitate efficient in vivo delivery.
This study identifies a safe and efficient compact PE2 that enables flexible split-PE design to facilitate efficient delivery in vivo
The study identifies a safe and efficient compact PE2 that enables flexible split prime editor design to facilitate efficient in vivo delivery.
This study identifies a safe and efficient compact PE2 that enables flexible split-PE design to facilitate efficient delivery in vivo
The study identifies a safe and efficient compact PE2 that enables flexible split prime editor design to facilitate efficient in vivo delivery.
This study identifies a safe and efficient compact PE2 that enables flexible split-PE design to facilitate efficient delivery in vivo
The study identifies a safe and efficient compact PE2 that enables flexible split prime editor design to facilitate efficient in vivo delivery.
This study identifies a safe and efficient compact PE2 that enables flexible split-PE design to facilitate efficient delivery in vivo
The study identifies a safe and efficient compact PE2 that enables flexible split prime editor design to facilitate efficient in vivo delivery.
This study identifies a safe and efficient compact PE2 that enables flexible split-PE design to facilitate efficient delivery in vivo
The study identifies a safe and efficient compact PE2 that enables flexible split prime editor design to facilitate efficient in vivo delivery.
This study identifies a safe and efficient compact PE2 that enables flexible split-PE design to facilitate efficient delivery in vivo
The study identifies a safe and efficient compact PE2 that enables flexible split prime editor design to facilitate efficient in vivo delivery.
This study identifies a safe and efficient compact PE2 that enables flexible split-PE design to facilitate efficient delivery in vivo
A compact prime editor with complete deletion of the reverse transcriptase RNase H domain showed comparable editing to full-length prime editor.
we developed a compact PE with complete deletion of the RNase H domain of reverse transcriptase (RT), which showed comparable editing to full-length PE
A compact prime editor with complete deletion of the reverse transcriptase RNase H domain showed comparable editing to full-length prime editor.
we developed a compact PE with complete deletion of the RNase H domain of reverse transcriptase (RT), which showed comparable editing to full-length PE
A compact prime editor with complete deletion of the reverse transcriptase RNase H domain showed comparable editing to full-length prime editor.
we developed a compact PE with complete deletion of the RNase H domain of reverse transcriptase (RT), which showed comparable editing to full-length PE
A compact prime editor with complete deletion of the reverse transcriptase RNase H domain showed comparable editing to full-length prime editor.
we developed a compact PE with complete deletion of the RNase H domain of reverse transcriptase (RT), which showed comparable editing to full-length PE
A compact prime editor with complete deletion of the reverse transcriptase RNase H domain showed comparable editing to full-length prime editor.
we developed a compact PE with complete deletion of the RNase H domain of reverse transcriptase (RT), which showed comparable editing to full-length PE
A compact prime editor with complete deletion of the reverse transcriptase RNase H domain showed comparable editing to full-length prime editor.
we developed a compact PE with complete deletion of the RNase H domain of reverse transcriptase (RT), which showed comparable editing to full-length PE
A compact prime editor with complete deletion of the reverse transcriptase RNase H domain showed comparable editing to full-length prime editor.
we developed a compact PE with complete deletion of the RNase H domain of reverse transcriptase (RT), which showed comparable editing to full-length PE
The compact prime editor, unlike PE2, abolished binding to eRF1 and had minimal effect on stop codon readthrough.
The compact PE, but not PE2, abolished its binding to eRF1 and showed minimal effect on stop codon readthrough
The compact prime editor, unlike PE2, abolished binding to eRF1 and had minimal effect on stop codon readthrough.
The compact PE, but not PE2, abolished its binding to eRF1 and showed minimal effect on stop codon readthrough
The compact prime editor, unlike PE2, abolished binding to eRF1 and had minimal effect on stop codon readthrough.
The compact PE, but not PE2, abolished its binding to eRF1 and showed minimal effect on stop codon readthrough
The compact prime editor, unlike PE2, abolished binding to eRF1 and had minimal effect on stop codon readthrough.
The compact PE, but not PE2, abolished its binding to eRF1 and showed minimal effect on stop codon readthrough
The compact prime editor, unlike PE2, abolished binding to eRF1 and had minimal effect on stop codon readthrough.
The compact PE, but not PE2, abolished its binding to eRF1 and showed minimal effect on stop codon readthrough
The compact prime editor, unlike PE2, abolished binding to eRF1 and had minimal effect on stop codon readthrough.
The compact PE, but not PE2, abolished its binding to eRF1 and showed minimal effect on stop codon readthrough
The compact prime editor, unlike PE2, abolished binding to eRF1 and had minimal effect on stop codon readthrough.
The compact PE, but not PE2, abolished its binding to eRF1 and showed minimal effect on stop codon readthrough
Using the compact prime editor, the Cas9 Glu573 split site supported robust editing up to 93% of full-length prime editor.
Using compact PE, we tested the effect of 4 different Cas9 split sites and found that the Glu 573 split site supports robust editing (up to 93% of full-length PE)
editing relative to full-length PE 93 %
Using the compact prime editor, the Cas9 Glu573 split site supported robust editing up to 93% of full-length prime editor.
Using compact PE, we tested the effect of 4 different Cas9 split sites and found that the Glu 573 split site supports robust editing (up to 93% of full-length PE)
editing relative to full-length PE 93 %
Using the compact prime editor, the Cas9 Glu573 split site supported robust editing up to 93% of full-length prime editor.
Using compact PE, we tested the effect of 4 different Cas9 split sites and found that the Glu 573 split site supports robust editing (up to 93% of full-length PE)
editing relative to full-length PE 93 %
Using the compact prime editor, the Cas9 Glu573 split site supported robust editing up to 93% of full-length prime editor.
Using compact PE, we tested the effect of 4 different Cas9 split sites and found that the Glu 573 split site supports robust editing (up to 93% of full-length PE)
editing relative to full-length PE 93 %
Using the compact prime editor, the Cas9 Glu573 split site supported robust editing up to 93% of full-length prime editor.
Using compact PE, we tested the effect of 4 different Cas9 split sites and found that the Glu 573 split site supports robust editing (up to 93% of full-length PE)
editing relative to full-length PE 93 %
Using the compact prime editor, the Cas9 Glu573 split site supported robust editing up to 93% of full-length prime editor.
Using compact PE, we tested the effect of 4 different Cas9 split sites and found that the Glu 573 split site supports robust editing (up to 93% of full-length PE)
editing relative to full-length PE 93 %
Using the compact prime editor, the Cas9 Glu573 split site supported robust editing up to 93% of full-length prime editor.
Using compact PE, we tested the effect of 4 different Cas9 split sites and found that the Glu 573 split site supports robust editing (up to 93% of full-length PE)
editing relative to full-length PE 93 %
Approval Evidence
1 source4 linked approval claimsfirst-pass slug compact-pe
Here, we developed a compact PE with complete deletion of the RNase H domain of reverse transcriptase (RT)
Source:
application potentialsupports
The study identifies a safe and efficient compact PE2 that enables flexible split prime editor design to facilitate efficient in vivo delivery.
This study identifies a safe and efficient compact PE2 that enables flexible split-PE design to facilitate efficient delivery in vivo
Source:
engineering resultsupports
A compact prime editor with complete deletion of the reverse transcriptase RNase H domain showed comparable editing to full-length prime editor.
we developed a compact PE with complete deletion of the RNase H domain of reverse transcriptase (RT), which showed comparable editing to full-length PE
Source:
mechanistic safetysupports
The compact prime editor, unlike PE2, abolished binding to eRF1 and had minimal effect on stop codon readthrough.
The compact PE, but not PE2, abolished its binding to eRF1 and showed minimal effect on stop codon readthrough
Source:
performance comparisonsupports
Using the compact prime editor, the Cas9 Glu573 split site supported robust editing up to 93% of full-length prime editor.
Using compact PE, we tested the effect of 4 different Cas9 split sites and found that the Glu 573 split site supports robust editing (up to 93% of full-length PE)
Source:
Comparisons
Source-backed strengths
The reported compact PE retained editing activity comparable to full-length PE2 despite complete deletion of the reverse transcriptase RNase H domain. The study further reports abolished eRF1 binding, minimal effect on stop codon readthrough, and utility for flexible split prime editor design aimed at efficient in vivo delivery.
Source:
we developed a compact PE with complete deletion of the RNase H domain of reverse transcriptase (RT), which showed comparable editing to full-length PE
Source:
Using compact PE, we tested the effect of 4 different Cas9 split sites and found that the Glu 573 split site supports robust editing (up to 93% of full-length PE)
Ranked Citations
- 1.