Source 1review2023Journal of Molecular Biology
Toolkit/Computational methods for LOV-based optogenetic tool development
Computational methods for LOV-based optogenetic tool development
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Computational methods for LOV-based optogenetic tool development are design-enabling approaches used in the ongoing development of Light-Oxygen-Voltage domain-based optogenetic systems. The cited evidence supports a role for computational methods as one of several factors advancing LOV-based tools for light-controlled biological regulation.
Usefulness & Problems
Why this is useful
These methods are useful because they contribute to the development pipeline for LOV-based optogenetic tools. The evidence specifically places computational methods alongside structural biology, spectroscopy, and synthetic biology as drivers of progress in this area.
Source:
Moreover, these domains have been identified across all kingdoms of life. LOV domains are versatile photoreceptors that play critical roles in cellular signaling and environmental adaptation
Problem solved
This approach helps address the challenge of developing and improving LOV-based optogenetic systems. The available evidence does not specify which design problems, performance bottlenecks, or computational tasks are solved by particular methods.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete computational method used to design, rank, or analyze an engineered system.
Mechanisms
light sensing by lov domainsTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
The supplied evidence identifies LOV domains as the biological basis of the tool class and indicates that these domains are used in optogenetic development. However, no practical implementation details are provided for software, construct design, expression context, chromophore handling, or experimental workflow.
The evidence does not describe any specific computational framework, algorithm, benchmark, or validated design outcome. It also does not report quantitative performance, target proteins, wavelengths, or independent demonstrations of a particular computationally designed LOV-based tool.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Development of LOV-based optogenetic tools is being driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology.
The ongoing development of LOV-based optogenetic tools, driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology
Development of LOV-based optogenetic tools is being driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology.
The ongoing development of LOV-based optogenetic tools, driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology
Development of LOV-based optogenetic tools is being driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology.
The ongoing development of LOV-based optogenetic tools, driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology
Development of LOV-based optogenetic tools is being driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology.
The ongoing development of LOV-based optogenetic tools, driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology
Development of LOV-based optogenetic tools is being driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology.
The ongoing development of LOV-based optogenetic tools, driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology
Development of LOV-based optogenetic tools is being driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology.
The ongoing development of LOV-based optogenetic tools, driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology
Development of LOV-based optogenetic tools is being driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology.
The ongoing development of LOV-based optogenetic tools, driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology
LOV domains are versatile photoreceptors involved in cellular signaling and environmental adaptation across kingdoms of life.
Moreover, these domains have been identified across all kingdoms of life. LOV domains are versatile photoreceptors that play critical roles in cellular signaling and environmental adaptation
LOV-based optogenetic tools have potential to enable novel therapeutic strategies.
has the potential to revolutionize the study of biological systems and enable the development of novel therapeutic strategies
LOV-based optogenetic tools have potential to enable novel therapeutic strategies.
has the potential to revolutionize the study of biological systems and enable the development of novel therapeutic strategies
LOV-based optogenetic tools have potential to enable novel therapeutic strategies.
has the potential to revolutionize the study of biological systems and enable the development of novel therapeutic strategies
LOV-based optogenetic tools have potential to enable novel therapeutic strategies.
has the potential to revolutionize the study of biological systems and enable the development of novel therapeutic strategies
LOV-based optogenetic tools have potential to enable novel therapeutic strategies.
has the potential to revolutionize the study of biological systems and enable the development of novel therapeutic strategies
LOV-based optogenetic tools have potential to enable novel therapeutic strategies.
has the potential to revolutionize the study of biological systems and enable the development of novel therapeutic strategies
LOV-based optogenetic tools have potential to enable novel therapeutic strategies.
has the potential to revolutionize the study of biological systems and enable the development of novel therapeutic strategies
LOV domains use flavin nucleotides as cofactors and undergo blue-light-induced structural rearrangements that activate an effector domain.
LOV domains utilize flavin nucleotides as co-factors and undergo structural rearrangements upon exposure to blue light, which activates an effector domain that executes the final output of the photoreaction.
LOV domains can be used to noninvasively sense and control intracellular processes with high spatiotemporal precision, making them suitable for optogenetics.
they can noninvasively sense and control intracellular processes with high spatiotemporal precision, making them ideal candidates for use in optogenetics
LOV domains can be used to noninvasively sense and control intracellular processes with high spatiotemporal precision, making them suitable for optogenetics.
they can noninvasively sense and control intracellular processes with high spatiotemporal precision, making them ideal candidates for use in optogenetics
LOV domains can be used to noninvasively sense and control intracellular processes with high spatiotemporal precision, making them suitable for optogenetics.
they can noninvasively sense and control intracellular processes with high spatiotemporal precision, making them ideal candidates for use in optogenetics
LOV domains can be used to noninvasively sense and control intracellular processes with high spatiotemporal precision, making them suitable for optogenetics.
they can noninvasively sense and control intracellular processes with high spatiotemporal precision, making them ideal candidates for use in optogenetics
LOV domains can be used to noninvasively sense and control intracellular processes with high spatiotemporal precision, making them suitable for optogenetics.
they can noninvasively sense and control intracellular processes with high spatiotemporal precision, making them ideal candidates for use in optogenetics
LOV domains can be used to noninvasively sense and control intracellular processes with high spatiotemporal precision, making them suitable for optogenetics.
they can noninvasively sense and control intracellular processes with high spatiotemporal precision, making them ideal candidates for use in optogenetics
LOV domains can be used to noninvasively sense and control intracellular processes with high spatiotemporal precision, making them suitable for optogenetics.
they can noninvasively sense and control intracellular processes with high spatiotemporal precision, making them ideal candidates for use in optogenetics
Approval Evidence
1 source1 linked approval claimfirst-pass slug computational-methods-for-lov-based-optogenetic-tool-development
The ongoing development of LOV-based optogenetic tools, driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology...
Source:
development driversupports
Development of LOV-based optogenetic tools is being driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology.
The ongoing development of LOV-based optogenetic tools, driven by advances in structural biology, spectroscopy, computational methods, and synthetic biology
Source:
Comparisons
Source-backed strengths
A key strength supported by the evidence is that computational methods are recognized as an active driver of ongoing LOV-tool development. The source also frames LOV domains as versatile photoreceptors involved in cellular signaling and environmental adaptation across kingdoms of life, which supports the relevance of developing tools around this scaffold.
Ranked Citations
- 1.