Source 1review2024ACS Synthetic Biology
Toolkit/Corelets
Corelets
Also known as: Corelets
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Corelets is a biomimetic optogenetic multicomponent switch designed to control intracellular phase separation with light-activated multivalent seeds. It was used to induce condensate formation and to map intracellular phase behavior, including regimes of nucleation-and-growth and spinodal decomposition.
Usefulness & Problems
Why this is useful
Corelets is useful for experimentally controlling and interrogating liquid-liquid phase separation inside living cells. The system enables mapping of intracellular phase diagrams and analysis of how localized seeding can drive condensate formation under conditions where global protein abundance is insufficient for bulk phase separation.
Source:
Here we introduce a biomimetic optogenetic system, “Corelets,”
Problem solved
Corelets addresses the problem of how to trigger and study intracellular condensate formation with spatial and mechanistic control. It also helps resolve whether phase separation occurs in a given intracellular state and whether the transition follows nucleation and growth or spinodal decomposition.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
diffusive captureligand sequestration to slowly diffusing nucleation centerslocalized liquid-liquid phase separationmultivalent seeding of phase separationoptogenetic light activationTechniques
Selection / EnrichmentTarget processes
selectionInput: Light
Implementation Constraints
The available evidence supports that Corelets is a light-activated, multicomponent, biomimetic system built around multivalent seeds for intracellular phase-separation control. The provided material does not specify the optogenetic module, protein domains, expression context, or delivery requirements.
The supplied evidence does not provide detailed quantitative performance metrics, construct composition, activation wavelength, or cross-system validation. Independent replication is not documented in the provided material, and the evidence is centered on a single 2018 study.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2primary paper2018
Source 3review2024Small Methods
Source 4review2024Journal of Molecular Biology
Ranked Claims
The review discusses design of photoactive complex coacervate protocells in laboratory settings using photochromic molecules such as azobenzene and diarylethene.
The design of photoactive complex coacervate protocells in laboratory settings by utilizing photochromic molecules such as azobenzene and diarylethene is further discussed.
optoDroplet, Corelet, PixELL, and CasDrop are highlighted as intracellular systems that enable photo-mediated control over biomolecular condensation.
Among these, the intracellular systems (i.e., optoDroplet, Corelet, PixELL, CasDrop, and other optogenetic systems) that enable the photo-mediated control over biomolecular condensation are highlighted.
The diffusive capture mechanism may allow cells to pattern condensates without requiring high global protein abundance and is likely used in diverse biological processes.
This diffusive capture mechanism liberates the cell from the constraints of global protein abundance and is likely exploited to pattern condensates associated with diverse biological processes.
The diffusive capture mechanism may allow cells to pattern condensates without requiring high global protein abundance and is likely used in diverse biological processes.
This diffusive capture mechanism liberates the cell from the constraints of global protein abundance and is likely exploited to pattern condensates associated with diverse biological processes.
The diffusive capture mechanism may allow cells to pattern condensates without requiring high global protein abundance and is likely used in diverse biological processes.
This diffusive capture mechanism liberates the cell from the constraints of global protein abundance and is likely exploited to pattern condensates associated with diverse biological processes.
The diffusive capture mechanism may allow cells to pattern condensates without requiring high global protein abundance and is likely used in diverse biological processes.
This diffusive capture mechanism liberates the cell from the constraints of global protein abundance and is likely exploited to pattern condensates associated with diverse biological processes.
The diffusive capture mechanism may allow cells to pattern condensates without requiring high global protein abundance and is likely used in diverse biological processes.
This diffusive capture mechanism liberates the cell from the constraints of global protein abundance and is likely exploited to pattern condensates associated with diverse biological processes.
The diffusive capture mechanism may allow cells to pattern condensates without requiring high global protein abundance and is likely used in diverse biological processes.
This diffusive capture mechanism liberates the cell from the constraints of global protein abundance and is likely exploited to pattern condensates associated with diverse biological processes.
The diffusive capture mechanism may allow cells to pattern condensates without requiring high global protein abundance and is likely used in diverse biological processes.
This diffusive capture mechanism liberates the cell from the constraints of global protein abundance and is likely exploited to pattern condensates associated with diverse biological processes.
Corelets were used to map intracellular phase diagrams that determine whether phase separation occurs and whether it proceeds by nucleation and growth or spinodal decomposition.
utilize its rapid and quantitative tunability to map the first full intracellular phase diagrams, which dictate whether phase separation occurs, and if so by nucleation and growth or spinodal decomposition
Corelets were used to map intracellular phase diagrams that determine whether phase separation occurs and whether it proceeds by nucleation and growth or spinodal decomposition.
utilize its rapid and quantitative tunability to map the first full intracellular phase diagrams, which dictate whether phase separation occurs, and if so by nucleation and growth or spinodal decomposition
Corelets were used to map intracellular phase diagrams that determine whether phase separation occurs and whether it proceeds by nucleation and growth or spinodal decomposition.
utilize its rapid and quantitative tunability to map the first full intracellular phase diagrams, which dictate whether phase separation occurs, and if so by nucleation and growth or spinodal decomposition
Corelets were used to map intracellular phase diagrams that determine whether phase separation occurs and whether it proceeds by nucleation and growth or spinodal decomposition.
utilize its rapid and quantitative tunability to map the first full intracellular phase diagrams, which dictate whether phase separation occurs, and if so by nucleation and growth or spinodal decomposition
Corelets were used to map intracellular phase diagrams that determine whether phase separation occurs and whether it proceeds by nucleation and growth or spinodal decomposition.
utilize its rapid and quantitative tunability to map the first full intracellular phase diagrams, which dictate whether phase separation occurs, and if so by nucleation and growth or spinodal decomposition
Corelets were used to map intracellular phase diagrams that determine whether phase separation occurs and whether it proceeds by nucleation and growth or spinodal decomposition.
utilize its rapid and quantitative tunability to map the first full intracellular phase diagrams, which dictate whether phase separation occurs, and if so by nucleation and growth or spinodal decomposition
Corelets were used to map intracellular phase diagrams that determine whether phase separation occurs and whether it proceeds by nucleation and growth or spinodal decomposition.
utilize its rapid and quantitative tunability to map the first full intracellular phase diagrams, which dictate whether phase separation occurs, and if so by nucleation and growth or spinodal decomposition
Experiments and simulations support that sequestering protein ligands to slowly diffusing nucleation centers can shift cells into a phase-diagram region that produces localized phase separation even when global intracellular concentrations are insufficient for global phase separation.
both experiments and simulations show that while intracellular concentrations may be insufficient for global phase separation, sequestering protein ligands to slowly diffusing nucleation centers can move the cell into a different region of the phase diagram, resulting in localized phase separation
Experiments and simulations support that sequestering protein ligands to slowly diffusing nucleation centers can shift cells into a phase-diagram region that produces localized phase separation even when global intracellular concentrations are insufficient for global phase separation.
both experiments and simulations show that while intracellular concentrations may be insufficient for global phase separation, sequestering protein ligands to slowly diffusing nucleation centers can move the cell into a different region of the phase diagram, resulting in localized phase separation
Experiments and simulations support that sequestering protein ligands to slowly diffusing nucleation centers can shift cells into a phase-diagram region that produces localized phase separation even when global intracellular concentrations are insufficient for global phase separation.
both experiments and simulations show that while intracellular concentrations may be insufficient for global phase separation, sequestering protein ligands to slowly diffusing nucleation centers can move the cell into a different region of the phase diagram, resulting in localized phase separation
Experiments and simulations support that sequestering protein ligands to slowly diffusing nucleation centers can shift cells into a phase-diagram region that produces localized phase separation even when global intracellular concentrations are insufficient for global phase separation.
both experiments and simulations show that while intracellular concentrations may be insufficient for global phase separation, sequestering protein ligands to slowly diffusing nucleation centers can move the cell into a different region of the phase diagram, resulting in localized phase separation
Experiments and simulations support that sequestering protein ligands to slowly diffusing nucleation centers can shift cells into a phase-diagram region that produces localized phase separation even when global intracellular concentrations are insufficient for global phase separation.
both experiments and simulations show that while intracellular concentrations may be insufficient for global phase separation, sequestering protein ligands to slowly diffusing nucleation centers can move the cell into a different region of the phase diagram, resulting in localized phase separation
Experiments and simulations support that sequestering protein ligands to slowly diffusing nucleation centers can shift cells into a phase-diagram region that produces localized phase separation even when global intracellular concentrations are insufficient for global phase separation.
both experiments and simulations show that while intracellular concentrations may be insufficient for global phase separation, sequestering protein ligands to slowly diffusing nucleation centers can move the cell into a different region of the phase diagram, resulting in localized phase separation
Experiments and simulations support that sequestering protein ligands to slowly diffusing nucleation centers can shift cells into a phase-diagram region that produces localized phase separation even when global intracellular concentrations are insufficient for global phase separation.
both experiments and simulations show that while intracellular concentrations may be insufficient for global phase separation, sequestering protein ligands to slowly diffusing nucleation centers can move the cell into a different region of the phase diagram, resulting in localized phase separation
The paper introduces Corelets as a biomimetic optogenetic system.
Here we introduce a biomimetic optogenetic system, “Corelets,”
The paper introduces Corelets as a biomimetic optogenetic system.
Here we introduce a biomimetic optogenetic system, “Corelets,”
The paper introduces Corelets as a biomimetic optogenetic system.
Here we introduce a biomimetic optogenetic system, “Corelets,”
The paper introduces Corelets as a biomimetic optogenetic system.
Here we introduce a biomimetic optogenetic system, “Corelets,”
The paper introduces Corelets as a biomimetic optogenetic system.
Here we introduce a biomimetic optogenetic system, “Corelets,”
The paper introduces Corelets as a biomimetic optogenetic system.
Here we introduce a biomimetic optogenetic system, “Corelets,”
The paper introduces Corelets as a biomimetic optogenetic system.
Here we introduce a biomimetic optogenetic system, “Corelets,”
Approval Evidence
4 sources5 linked approval claimsfirst-pass slugs corelet, corelets
Additional high-signal enrichment leads cluster into: (1) foundational optogenetic condensate tool papers, especially Cry2-based optoDroplets and ferritin/iLID/SspB-based Corelets
Source:
Among these, the intracellular systems (i.e., optoDroplet, Corelet, PixELL, CasDrop, and other optogenetic systems) that enable the photo-mediated control over biomolecular condensation are highlighted.
Source:
High-signal enrichment leads cluster around four core tool families explicitly discussed across primary literature: CRY2-based optoDroplets, CRISPR-targeted CasDrop, ferritin-based Corelets, and PixD/PixE-based PixELLs.
Source:
Here we introduce a biomimetic optogenetic system, “Corelets,”
Source:
tool class membershipsupports
optoDroplet, Corelet, PixELL, and CasDrop are highlighted as intracellular systems that enable photo-mediated control over biomolecular condensation.
Among these, the intracellular systems (i.e., optoDroplet, Corelet, PixELL, CasDrop, and other optogenetic systems) that enable the photo-mediated control over biomolecular condensation are highlighted.
Source:
biological implicationsupports
The diffusive capture mechanism may allow cells to pattern condensates without requiring high global protein abundance and is likely used in diverse biological processes.
This diffusive capture mechanism liberates the cell from the constraints of global protein abundance and is likely exploited to pattern condensates associated with diverse biological processes.
Source:
mapping capabilitysupports
Corelets were used to map intracellular phase diagrams that determine whether phase separation occurs and whether it proceeds by nucleation and growth or spinodal decomposition.
utilize its rapid and quantitative tunability to map the first full intracellular phase diagrams, which dictate whether phase separation occurs, and if so by nucleation and growth or spinodal decomposition
Source:
mechanistic modelsupports
Experiments and simulations support that sequestering protein ligands to slowly diffusing nucleation centers can shift cells into a phase-diagram region that produces localized phase separation even when global intracellular concentrations are insufficient for global phase separation.
both experiments and simulations show that while intracellular concentrations may be insufficient for global phase separation, sequestering protein ligands to slowly diffusing nucleation centers can move the cell into a different region of the phase diagram, resulting in localized phase separation
Source:
tool introductionsupports
The paper introduces Corelets as a biomimetic optogenetic system.
Here we introduce a biomimetic optogenetic system, “Corelets,”
Source:
Comparisons
Source-backed strengths
Source literature states that Corelets enabled intracellular phase-diagram mapping and mechanistic analysis of local versus global phase behavior. Experiments and simulations supported a diffusive-capture model in which ligand sequestration to slowly diffusing nucleation centers shifts cells into a regime that produces localized phase separation.
Ranked Citations
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Extracted from this source document.