Source 1primary paper2019
Toolkit/CreLite
CreLite
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
CreLite is an optogenetically controlled Cre/loxP recombination system reported in developing zebrafish embryos. It uses split Cre recombinase halves fused to the red light-inducible partners PhyB and PIF6, so that 660 nm illumination in the presence of phycocyanobilin (PCB) restores Cre activity.
Usefulness & Problems
Why this is useful
CreLite enables light-gated control of Cre/loxP recombination with red light in developing zebrafish embryos. In transgenic embryos carrying a Cre-dependent multicolor fluorescent reporter, this system produced detectable Cre activity after red-light exposure, supporting spatially and temporally controlled recombination assays in vivo.
Source:
Red-light exposure of transgenic zebrafish embryos harboring a Cre-dependent multi-color fluorescent protein reporter ( ubi:zebrabow ) injected with CreLite mRNAs and PCB, resulted in Cre activity as measured by the generation of multi-spectral cell labeling in various tissues, including heart, skeletal muscle and epithelium.
Source:
Here we present CreLite, an optogenetically-controlled Cre system using red light in developing zebrafish embryos.
Source:
We show that CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
Problem solved
CreLite addresses the problem of making Cre recombinase activity conditional on an optical input rather than constitutive expression alone. Specifically, it provides a red light- and PCB-dependent method to trigger recombination in developing zebrafish embryos.
Source:
Red-light exposure of transgenic zebrafish embryos harboring a Cre-dependent multi-color fluorescent protein reporter ( ubi:zebrabow ) injected with CreLite mRNAs and PCB, resulted in Cre activity as measured by the generation of multi-spectral cell labeling in various tissues, including heart, skeletal muscle and epithelium.
Source:
We show that CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
No technique tags yet.
Target processes
recombinationInput: Light
Implementation Constraints
CreLite is implemented by splitting Cre recombinase into inactive halves and fusing them to PhyB and PIF6, specifically as PhyB-CreC and PIF6-CreN. Reported use involved injection of CreLite mRNAs and PCB into transgenic zebrafish embryos, followed by 660 nm red-light exposure and readout with a Cre-dependent multicolor fluorescent reporter.
The supplied evidence supports use in developing zebrafish embryos but does not establish performance in other organisms or cell types. The system also requires exogenous PCB in addition to red-light exposure, and the evidence set does not provide quantitative benchmarking, background activity measurements, or independent replication.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
In transgenic zebrafish embryos carrying a Cre-dependent multi-color fluorescent reporter, red-light exposure after injection with CreLite mRNAs and PCB resulted in Cre activity measured by multi-spectral cell labeling in heart, skeletal muscle, and epithelium.
Red-light exposure of transgenic zebrafish embryos harboring a Cre-dependent multi-color fluorescent protein reporter ( ubi:zebrabow ) injected with CreLite mRNAs and PCB, resulted in Cre activity as measured by the generation of multi-spectral cell labeling in various tissues, including heart, skeletal muscle and epithelium.
In transgenic zebrafish embryos carrying a Cre-dependent multi-color fluorescent reporter, red-light exposure after injection with CreLite mRNAs and PCB resulted in Cre activity measured by multi-spectral cell labeling in heart, skeletal muscle, and epithelium.
Red-light exposure of transgenic zebrafish embryos harboring a Cre-dependent multi-color fluorescent protein reporter ( ubi:zebrabow ) injected with CreLite mRNAs and PCB, resulted in Cre activity as measured by the generation of multi-spectral cell labeling in various tissues, including heart, skeletal muscle and epithelium.
In transgenic zebrafish embryos carrying a Cre-dependent multi-color fluorescent reporter, red-light exposure after injection with CreLite mRNAs and PCB resulted in Cre activity measured by multi-spectral cell labeling in heart, skeletal muscle, and epithelium.
Red-light exposure of transgenic zebrafish embryos harboring a Cre-dependent multi-color fluorescent protein reporter ( ubi:zebrabow ) injected with CreLite mRNAs and PCB, resulted in Cre activity as measured by the generation of multi-spectral cell labeling in various tissues, including heart, skeletal muscle and epithelium.
In transgenic zebrafish embryos carrying a Cre-dependent multi-color fluorescent reporter, red-light exposure after injection with CreLite mRNAs and PCB resulted in Cre activity measured by multi-spectral cell labeling in heart, skeletal muscle, and epithelium.
Red-light exposure of transgenic zebrafish embryos harboring a Cre-dependent multi-color fluorescent protein reporter ( ubi:zebrabow ) injected with CreLite mRNAs and PCB, resulted in Cre activity as measured by the generation of multi-spectral cell labeling in various tissues, including heart, skeletal muscle and epithelium.
In transgenic zebrafish embryos carrying a Cre-dependent multi-color fluorescent reporter, red-light exposure after injection with CreLite mRNAs and PCB resulted in Cre activity measured by multi-spectral cell labeling in heart, skeletal muscle, and epithelium.
Red-light exposure of transgenic zebrafish embryos harboring a Cre-dependent multi-color fluorescent protein reporter ( ubi:zebrabow ) injected with CreLite mRNAs and PCB, resulted in Cre activity as measured by the generation of multi-spectral cell labeling in various tissues, including heart, skeletal muscle and epithelium.
In transgenic zebrafish embryos carrying a Cre-dependent multi-color fluorescent reporter, red-light exposure after injection with CreLite mRNAs and PCB resulted in Cre activity measured by multi-spectral cell labeling in heart, skeletal muscle, and epithelium.
Red-light exposure of transgenic zebrafish embryos harboring a Cre-dependent multi-color fluorescent protein reporter ( ubi:zebrabow ) injected with CreLite mRNAs and PCB, resulted in Cre activity as measured by the generation of multi-spectral cell labeling in various tissues, including heart, skeletal muscle and epithelium.
In transgenic zebrafish embryos carrying a Cre-dependent multi-color fluorescent reporter, red-light exposure after injection with CreLite mRNAs and PCB resulted in Cre activity measured by multi-spectral cell labeling in heart, skeletal muscle, and epithelium.
Red-light exposure of transgenic zebrafish embryos harboring a Cre-dependent multi-color fluorescent protein reporter ( ubi:zebrabow ) injected with CreLite mRNAs and PCB, resulted in Cre activity as measured by the generation of multi-spectral cell labeling in various tissues, including heart, skeletal muscle and epithelium.
CreLite disables Cre by splitting Cre and fusing the inactive halves to the red light-inducible binding partners PhyB and PIF6.
Cre activity is disabled by splitting Cre and fusing the inactive halves with the Arabidopsis thaliana red light-inducible binding partners, PhyB and PIF6.
CreLite disables Cre by splitting Cre and fusing the inactive halves to the red light-inducible binding partners PhyB and PIF6.
Cre activity is disabled by splitting Cre and fusing the inactive halves with the Arabidopsis thaliana red light-inducible binding partners, PhyB and PIF6.
CreLite disables Cre by splitting Cre and fusing the inactive halves to the red light-inducible binding partners PhyB and PIF6.
Cre activity is disabled by splitting Cre and fusing the inactive halves with the Arabidopsis thaliana red light-inducible binding partners, PhyB and PIF6.
CreLite disables Cre by splitting Cre and fusing the inactive halves to the red light-inducible binding partners PhyB and PIF6.
Cre activity is disabled by splitting Cre and fusing the inactive halves with the Arabidopsis thaliana red light-inducible binding partners, PhyB and PIF6.
CreLite disables Cre by splitting Cre and fusing the inactive halves to the red light-inducible binding partners PhyB and PIF6.
Cre activity is disabled by splitting Cre and fusing the inactive halves with the Arabidopsis thaliana red light-inducible binding partners, PhyB and PIF6.
CreLite disables Cre by splitting Cre and fusing the inactive halves to the red light-inducible binding partners PhyB and PIF6.
Cre activity is disabled by splitting Cre and fusing the inactive halves with the Arabidopsis thaliana red light-inducible binding partners, PhyB and PIF6.
CreLite disables Cre by splitting Cre and fusing the inactive halves to the red light-inducible binding partners PhyB and PIF6.
Cre activity is disabled by splitting Cre and fusing the inactive halves with the Arabidopsis thaliana red light-inducible binding partners, PhyB and PIF6.
Red light at 660 nm in the presence of PCB brings PhyB-CreC and PIF6-CreN together to restore Cre activity.
Upon exposure to red light (660 nm) illumination, the PhyB-CreC and PIF6-CreN fusion proteins come together in the presence of PCB to restore Cre activity.
activation wavelength 660 nm
Red light at 660 nm in the presence of PCB brings PhyB-CreC and PIF6-CreN together to restore Cre activity.
Upon exposure to red light (660 nm) illumination, the PhyB-CreC and PIF6-CreN fusion proteins come together in the presence of PCB to restore Cre activity.
activation wavelength 660 nm
Red light at 660 nm in the presence of PCB brings PhyB-CreC and PIF6-CreN together to restore Cre activity.
Upon exposure to red light (660 nm) illumination, the PhyB-CreC and PIF6-CreN fusion proteins come together in the presence of PCB to restore Cre activity.
activation wavelength 660 nm
Red light at 660 nm in the presence of PCB brings PhyB-CreC and PIF6-CreN together to restore Cre activity.
Upon exposure to red light (660 nm) illumination, the PhyB-CreC and PIF6-CreN fusion proteins come together in the presence of PCB to restore Cre activity.
activation wavelength 660 nm
Red light at 660 nm in the presence of PCB brings PhyB-CreC and PIF6-CreN together to restore Cre activity.
Upon exposure to red light (660 nm) illumination, the PhyB-CreC and PIF6-CreN fusion proteins come together in the presence of PCB to restore Cre activity.
activation wavelength 660 nm
Red light at 660 nm in the presence of PCB brings PhyB-CreC and PIF6-CreN together to restore Cre activity.
Upon exposure to red light (660 nm) illumination, the PhyB-CreC and PIF6-CreN fusion proteins come together in the presence of PCB to restore Cre activity.
activation wavelength 660 nm
Red light at 660 nm in the presence of PCB brings PhyB-CreC and PIF6-CreN together to restore Cre activity.
Upon exposure to red light (660 nm) illumination, the PhyB-CreC and PIF6-CreN fusion proteins come together in the presence of PCB to restore Cre activity.
activation wavelength 660 nm
CreLite is an optogenetically controlled Cre system that uses red light in developing zebrafish embryos.
Here we present CreLite, an optogenetically-controlled Cre system using red light in developing zebrafish embryos.
CreLite is an optogenetically controlled Cre system that uses red light in developing zebrafish embryos.
Here we present CreLite, an optogenetically-controlled Cre system using red light in developing zebrafish embryos.
CreLite is an optogenetically controlled Cre system that uses red light in developing zebrafish embryos.
Here we present CreLite, an optogenetically-controlled Cre system using red light in developing zebrafish embryos.
CreLite is an optogenetically controlled Cre system that uses red light in developing zebrafish embryos.
Here we present CreLite, an optogenetically-controlled Cre system using red light in developing zebrafish embryos.
CreLite is an optogenetically controlled Cre system that uses red light in developing zebrafish embryos.
Here we present CreLite, an optogenetically-controlled Cre system using red light in developing zebrafish embryos.
CreLite is an optogenetically controlled Cre system that uses red light in developing zebrafish embryos.
Here we present CreLite, an optogenetically-controlled Cre system using red light in developing zebrafish embryos.
CreLite is an optogenetically controlled Cre system that uses red light in developing zebrafish embryos.
Here we present CreLite, an optogenetically-controlled Cre system using red light in developing zebrafish embryos.
CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
We show that CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
We show that CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
We show that CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
We show that CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
We show that CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
We show that CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
We show that CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
Approval Evidence
1 source5 linked approval claimsfirst-pass slug crelite
Here we present CreLite, an optogenetically-controlled Cre system using red light in developing zebrafish embryos.
Source:
application resultsupports
In transgenic zebrafish embryos carrying a Cre-dependent multi-color fluorescent reporter, red-light exposure after injection with CreLite mRNAs and PCB resulted in Cre activity measured by multi-spectral cell labeling in heart, skeletal muscle, and epithelium.
Red-light exposure of transgenic zebrafish embryos harboring a Cre-dependent multi-color fluorescent protein reporter ( ubi:zebrabow ) injected with CreLite mRNAs and PCB, resulted in Cre activity as measured by the generation of multi-spectral cell labeling in various tissues, including heart, skeletal muscle and epithelium.
Source:
mechanismsupports
CreLite disables Cre by splitting Cre and fusing the inactive halves to the red light-inducible binding partners PhyB and PIF6.
Cre activity is disabled by splitting Cre and fusing the inactive halves with the Arabidopsis thaliana red light-inducible binding partners, PhyB and PIF6.
Source:
mechanismsupports
Red light at 660 nm in the presence of PCB brings PhyB-CreC and PIF6-CreN together to restore Cre activity.
Upon exposure to red light (660 nm) illumination, the PhyB-CreC and PIF6-CreN fusion proteins come together in the presence of PCB to restore Cre activity.
Source:
tool descriptionsupports
CreLite is an optogenetically controlled Cre system that uses red light in developing zebrafish embryos.
Here we present CreLite, an optogenetically-controlled Cre system using red light in developing zebrafish embryos.
Source:
use casesupports
CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
We show that CreLite can be used for gene manipulations in whole embryos or small groups of cells at different stages of development.
Source:
Comparisons
Source-backed strengths
The system is activated by 660 nm red light and was validated in vivo in zebrafish embryos. Functional output was demonstrated by multispectral cell labeling in heart, skeletal muscle, and epithelium in a Cre-dependent reporter background after injection of CreLite mRNAs and PCB followed by red-light exposure.
Ranked Citations
- 1.