Source 1primary paper2025MED
Toolkit/CRIBOS
CRIBOS
Also known as: cell-free recombinase-integrated Boolean output system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
We present the cell-free recombinase-integrated Boolean output system (CRIBOS), a site-specific recombinase-based multiplex genetic circuit platform designed for cell-free environments.
Usefulness & Problems
No literature-backed usefulness or problem-fit explainer has been materialized for this record yet.
Published Workflows
Objective: Develop a site-specific recombinase-based multiplex genetic circuit platform for cell-free environments and demonstrate its use for multi-input multi-output logic, multiplex environmental sensing, and paper-based biological memory storage.
Why it works: The abstract presents CRIBOS as a site-specific recombinase-based multiplex circuit platform for cell-free environments, with aTF-based sensors providing environmental input sensing and a paper-based format providing portability, stability, and long-term information preservation.
site-specific recombinase-based logicintegration with allosteric transcription factor sensor inputsDNA-based biological memory storagecell-free gene expressionpaper-based deployment
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Techniques
Computational DesignTarget processes
recombinationtranscriptionValidation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Paper-based CRIBOS functions as a biological memory storage logic circuit device that can preserve DNA-based biological information for over 4 months with minimal resources, energy costs, and maintenance requirements.
information preservation duration 4 months
When combined with allosteric transcription factor-based sensors, CRIBOS circuits demonstrate multiplex environmental sensing.
Using CRIBOS, the authors built over 20 multi-input-multi-output circuits, including 2-input-2-output genetic circuits and a 2-input-4-output decoder.
number of multi input multi output circuits built 20
Implementing CRIBOS expands the application of multiplex Boolean logic gates from cellular systems to the cell-free environment.
CRIBOS is a site-specific recombinase-based multiplex genetic circuit platform designed for cell-free environments.
Approval Evidence
1 source4 linked approval claimsfirst-pass slug cribos
We present the cell-free recombinase-integrated Boolean output system (CRIBOS), a site-specific recombinase-based multiplex genetic circuit platform designed for cell-free environments.
Source:
applicationsupports
When combined with allosteric transcription factor-based sensors, CRIBOS circuits demonstrate multiplex environmental sensing.
Source:
engineering outputsupports
Using CRIBOS, the authors built over 20 multi-input-multi-output circuits, including 2-input-2-output genetic circuits and a 2-input-4-output decoder.
Source:
scope extensionsupports
Implementing CRIBOS expands the application of multiplex Boolean logic gates from cellular systems to the cell-free environment.
Source:
tool introductionsupports
CRIBOS is a site-specific recombinase-based multiplex genetic circuit platform designed for cell-free environments.
Source:
Comparisons
No literature-backed comparison notes have been materialized for this record yet.
Ranked Citations
- 1.