Source 1review2021Biology
Toolkit/CRISPR
CRISPR
Also known as: clustered regularly interspaced short palindromic repeats, clustered regularly interspaced short palindromic repeat sequences
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
CRISPR is a widely used engineering method for targeted RNA and DNA manipulation across multiple organisms. The cited review highlights its use for viral genome manipulation, including gene knock-in and gene knock-out, and for precise diagnosis of viral infections.
Usefulness & Problems
Why this is useful
CRISPR is useful because it enables targeted manipulation of viral genomes and supports both experimental perturbation and diagnostic applications. The cited literature specifically emphasizes applications in SARS-CoV-2, HIV-1, and vaccinia virus, as well as identification of significant genes involved in virus-host interactions.
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Furthermore, we have emphasized the application of CRISPR technology in virus diagnosis and in finding significant genes involved in virus-host interactions.
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This approach has provided an unprecedented opportunity for creating simple, inexpensive, specific, targeted, accurate, and practical manipulations of viruses, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), human immunodeficiency virus-1 (HIV-1), and vaccinia virus.
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By 2013, CRISPR/Cas9 systems had been engineered to allow gene editing in mammalian cells.
Problem solved
CRISPR helps solve the problem of making targeted and effective changes to viral nucleic acids for editing and recombination-related applications. It also addresses the need for precise viral diagnostics and for functional interrogation of genes involved in virus-host interactions.
Source:
Furthermore, we have emphasized the application of CRISPR technology in virus diagnosis and in finding significant genes involved in virus-host interactions.
Source:
This approach has provided an unprecedented opportunity for creating simple, inexpensive, specific, targeted, accurate, and practical manipulations of viruses, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), human immunodeficiency virus-1 (HIV-1), and vaccinia virus.
Source:
By 2013, CRISPR/Cas9 systems had been engineered to allow gene editing in mammalian cells.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Techniques
Computational DesignTarget processes
editingrecombinationImplementation Constraints
The available evidence supports that CRISPR performance depends on scientifically valid system design for effective and accurate viral manipulation. The supplied sources do not provide specific implementation details such as nuclease variants, guide architecture, delivery systems, cofactors, or expression platforms.
The supplied evidence notes that CRISPR applications are associated with unintended off-target and on-target alterations. The review also states that a valid and scientifically designed CRISPR system is critical for more effective and accurate viral changes, indicating design sensitivity.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2review2022Cells
Ranked Claims
The review emphasizes CRISPR applications in virus diagnosis and in identifying significant genes involved in virus-host interactions.
Furthermore, we have emphasized the application of CRISPR technology in virus diagnosis and in finding significant genes involved in virus-host interactions.
The review states that CRISPR enables targeted manipulation of viral genomes, including examples involving SARS-CoV-2, HIV-1, and vaccinia virus.
This approach has provided an unprecedented opportunity for creating simple, inexpensive, specific, targeted, accurate, and practical manipulations of viruses, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), human immunodeficiency virus-1 (HIV-1), and vaccinia virus.
The review describes CRISPR as a widely used tool for RNA and DNA manipulation in multiple organisms.
CRISPR (clustered regularly interspaced short palindromic repeats) is becoming one of the most functional and widely used tools for RNA and DNA manipulation in multiple organisms.
The review states that a valid and scientifically designed CRISPR system is critical for more effective and accurate viral changes.
Nevertheless, a valid and scientifically designed CRISPR system is critical to make more effective and accurate changes in viruses.
The review states that CRISPR can be used for effective and precise diagnosis of viral infections.
Furthermore, this method can be used to make an effective and precise diagnosis of viral infections.
The review focuses on effective design of sgRNA and on gene knock-in and gene knock-out strategies for virus-targeted manipulation.
In this review, we have focused on the best and the most effective ways to design sgRNA, gene knock-in(s), and gene knock-out(s) for virus-targeted manipulation.
The review focuses on effective design of sgRNA and on gene knock-in and gene knock-out strategies for virus-targeted manipulation.
In this review, we have focused on the best and the most effective ways to design sgRNA, gene knock-in(s), and gene knock-out(s) for virus-targeted manipulation.
The review focuses on effective design of sgRNA and on gene knock-in and gene knock-out strategies for virus-targeted manipulation.
In this review, we have focused on the best and the most effective ways to design sgRNA, gene knock-in(s), and gene knock-out(s) for virus-targeted manipulation.
The review focuses on effective design of sgRNA and on gene knock-in and gene knock-out strategies for virus-targeted manipulation.
In this review, we have focused on the best and the most effective ways to design sgRNA, gene knock-in(s), and gene knock-out(s) for virus-targeted manipulation.
The review focuses on effective design of sgRNA and on gene knock-in and gene knock-out strategies for virus-targeted manipulation.
In this review, we have focused on the best and the most effective ways to design sgRNA, gene knock-in(s), and gene knock-out(s) for virus-targeted manipulation.
The review focuses on effective design of sgRNA and on gene knock-in and gene knock-out strategies for virus-targeted manipulation.
In this review, we have focused on the best and the most effective ways to design sgRNA, gene knock-in(s), and gene knock-out(s) for virus-targeted manipulation.
The review focuses on effective design of sgRNA and on gene knock-in and gene knock-out strategies for virus-targeted manipulation.
In this review, we have focused on the best and the most effective ways to design sgRNA, gene knock-in(s), and gene knock-out(s) for virus-targeted manipulation.
By 2013, CRISPR/Cas9 systems had been engineered to allow gene editing in mammalian cells.
By 2013, CRISPR/Cas9 systems had been engineered to allow gene editing in mammalian cells.
By 2013, CRISPR/Cas9 systems had been engineered to allow gene editing in mammalian cells.
By 2013, CRISPR/Cas9 systems had been engineered to allow gene editing in mammalian cells.
By 2013, CRISPR/Cas9 systems had been engineered to allow gene editing in mammalian cells.
By 2013, CRISPR/Cas9 systems had been engineered to allow gene editing in mammalian cells.
By 2013, CRISPR/Cas9 systems had been engineered to allow gene editing in mammalian cells.
By 2013, CRISPR/Cas9 systems had been engineered to allow gene editing in mammalian cells.
By 2013, CRISPR/Cas9 systems had been engineered to allow gene editing in mammalian cells.
By 2013, CRISPR/Cas9 systems had been engineered to allow gene editing in mammalian cells.
By 2013, CRISPR/Cas9 systems had been engineered to allow gene editing in mammalian cells.
By 2013, CRISPR/Cas9 systems had been engineered to allow gene editing in mammalian cells.
By 2013, CRISPR/Cas9 systems had been engineered to allow gene editing in mammalian cells.
By 2013, CRISPR/Cas9 systems had been engineered to allow gene editing in mammalian cells.
CRISPR/Cas9 and related systems are described as designer nucleases of choice because of ease of design, low cytotoxicity, and increased efficiency.
The ease of design, low cytotoxicity, and increased efficiency have made CRISPR/Cas9 and its related systems the designer nucleases of choice for many.
CRISPR/Cas9 and related systems are described as designer nucleases of choice because of ease of design, low cytotoxicity, and increased efficiency.
The ease of design, low cytotoxicity, and increased efficiency have made CRISPR/Cas9 and its related systems the designer nucleases of choice for many.
CRISPR/Cas9 and related systems are described as designer nucleases of choice because of ease of design, low cytotoxicity, and increased efficiency.
The ease of design, low cytotoxicity, and increased efficiency have made CRISPR/Cas9 and its related systems the designer nucleases of choice for many.
CRISPR/Cas9 and related systems are described as designer nucleases of choice because of ease of design, low cytotoxicity, and increased efficiency.
The ease of design, low cytotoxicity, and increased efficiency have made CRISPR/Cas9 and its related systems the designer nucleases of choice for many.
CRISPR/Cas9 and related systems are described as designer nucleases of choice because of ease of design, low cytotoxicity, and increased efficiency.
The ease of design, low cytotoxicity, and increased efficiency have made CRISPR/Cas9 and its related systems the designer nucleases of choice for many.
CRISPR/Cas9 and related systems are described as designer nucleases of choice because of ease of design, low cytotoxicity, and increased efficiency.
The ease of design, low cytotoxicity, and increased efficiency have made CRISPR/Cas9 and its related systems the designer nucleases of choice for many.
CRISPR/Cas9 and related systems are described as designer nucleases of choice because of ease of design, low cytotoxicity, and increased efficiency.
The ease of design, low cytotoxicity, and increased efficiency have made CRISPR/Cas9 and its related systems the designer nucleases of choice for many.
ZFN and TALEN provided sequence-specific gene-editing capacity but their broad utility was limited by laborious nuclease design and synthesis, limited target choices, and poor editing efficiency.
While these approaches provided sequence-specific gene-editing capacity, the laborious process of designing and synthesizing recombinant nucleases to recognize a specific target sequence, combined with limited target choices and poor editing efficiency, ultimately minimized the broad utility of these systems.
ZFN and TALEN provided sequence-specific gene-editing capacity but their broad utility was limited by laborious nuclease design and synthesis, limited target choices, and poor editing efficiency.
While these approaches provided sequence-specific gene-editing capacity, the laborious process of designing and synthesizing recombinant nucleases to recognize a specific target sequence, combined with limited target choices and poor editing efficiency, ultimately minimized the broad utility of these systems.
ZFN and TALEN provided sequence-specific gene-editing capacity but their broad utility was limited by laborious nuclease design and synthesis, limited target choices, and poor editing efficiency.
While these approaches provided sequence-specific gene-editing capacity, the laborious process of designing and synthesizing recombinant nucleases to recognize a specific target sequence, combined with limited target choices and poor editing efficiency, ultimately minimized the broad utility of these systems.
ZFN and TALEN provided sequence-specific gene-editing capacity but their broad utility was limited by laborious nuclease design and synthesis, limited target choices, and poor editing efficiency.
While these approaches provided sequence-specific gene-editing capacity, the laborious process of designing and synthesizing recombinant nucleases to recognize a specific target sequence, combined with limited target choices and poor editing efficiency, ultimately minimized the broad utility of these systems.
ZFN and TALEN provided sequence-specific gene-editing capacity but their broad utility was limited by laborious nuclease design and synthesis, limited target choices, and poor editing efficiency.
While these approaches provided sequence-specific gene-editing capacity, the laborious process of designing and synthesizing recombinant nucleases to recognize a specific target sequence, combined with limited target choices and poor editing efficiency, ultimately minimized the broad utility of these systems.
ZFN and TALEN provided sequence-specific gene-editing capacity but their broad utility was limited by laborious nuclease design and synthesis, limited target choices, and poor editing efficiency.
While these approaches provided sequence-specific gene-editing capacity, the laborious process of designing and synthesizing recombinant nucleases to recognize a specific target sequence, combined with limited target choices and poor editing efficiency, ultimately minimized the broad utility of these systems.
ZFN and TALEN provided sequence-specific gene-editing capacity but their broad utility was limited by laborious nuclease design and synthesis, limited target choices, and poor editing efficiency.
While these approaches provided sequence-specific gene-editing capacity, the laborious process of designing and synthesizing recombinant nucleases to recognize a specific target sequence, combined with limited target choices and poor editing efficiency, ultimately minimized the broad utility of these systems.
CRISPR and Cas proteins were identified as part of a microbial adaptive immune system that targets phage DNA to fight bacteriophage reinfection.
CRISPR and the CRISPR-associated (Cas) proteins were identified as part of the microbial adaptive immune system, by targeting phage DNA, to fight bacteriophage reinfection.
CRISPR and Cas proteins were identified as part of a microbial adaptive immune system that targets phage DNA to fight bacteriophage reinfection.
CRISPR and the CRISPR-associated (Cas) proteins were identified as part of the microbial adaptive immune system, by targeting phage DNA, to fight bacteriophage reinfection.
CRISPR and Cas proteins were identified as part of a microbial adaptive immune system that targets phage DNA to fight bacteriophage reinfection.
CRISPR and the CRISPR-associated (Cas) proteins were identified as part of the microbial adaptive immune system, by targeting phage DNA, to fight bacteriophage reinfection.
CRISPR and Cas proteins were identified as part of a microbial adaptive immune system that targets phage DNA to fight bacteriophage reinfection.
CRISPR and the CRISPR-associated (Cas) proteins were identified as part of the microbial adaptive immune system, by targeting phage DNA, to fight bacteriophage reinfection.
CRISPR and Cas proteins were identified as part of a microbial adaptive immune system that targets phage DNA to fight bacteriophage reinfection.
CRISPR and the CRISPR-associated (Cas) proteins were identified as part of the microbial adaptive immune system, by targeting phage DNA, to fight bacteriophage reinfection.
CRISPR and Cas proteins were identified as part of a microbial adaptive immune system that targets phage DNA to fight bacteriophage reinfection.
CRISPR and the CRISPR-associated (Cas) proteins were identified as part of the microbial adaptive immune system, by targeting phage DNA, to fight bacteriophage reinfection.
CRISPR and Cas proteins were identified as part of a microbial adaptive immune system that targets phage DNA to fight bacteriophage reinfection.
CRISPR and the CRISPR-associated (Cas) proteins were identified as part of the microbial adaptive immune system, by targeting phage DNA, to fight bacteriophage reinfection.
The review discusses various CRISPR systems and their broad utility in genome manipulation, including how CRISPR-controlled modification of DNA repair genes has advanced understanding of genome stability mechanisms.
In this review, we discuss the various CRISPR systems and their broad utility in genome manipulation. We will explore how CRISPR-controlled modifications have advanced our understanding of the mechanisms of genome stability, using the modulation of DNA repair genes as examples.
The review discusses various CRISPR systems and their broad utility in genome manipulation, including how CRISPR-controlled modification of DNA repair genes has advanced understanding of genome stability mechanisms.
In this review, we discuss the various CRISPR systems and their broad utility in genome manipulation. We will explore how CRISPR-controlled modifications have advanced our understanding of the mechanisms of genome stability, using the modulation of DNA repair genes as examples.
The review discusses various CRISPR systems and their broad utility in genome manipulation, including how CRISPR-controlled modification of DNA repair genes has advanced understanding of genome stability mechanisms.
In this review, we discuss the various CRISPR systems and their broad utility in genome manipulation. We will explore how CRISPR-controlled modifications have advanced our understanding of the mechanisms of genome stability, using the modulation of DNA repair genes as examples.
The review discusses various CRISPR systems and their broad utility in genome manipulation, including how CRISPR-controlled modification of DNA repair genes has advanced understanding of genome stability mechanisms.
In this review, we discuss the various CRISPR systems and their broad utility in genome manipulation. We will explore how CRISPR-controlled modifications have advanced our understanding of the mechanisms of genome stability, using the modulation of DNA repair genes as examples.
The review discusses various CRISPR systems and their broad utility in genome manipulation, including how CRISPR-controlled modification of DNA repair genes has advanced understanding of genome stability mechanisms.
In this review, we discuss the various CRISPR systems and their broad utility in genome manipulation. We will explore how CRISPR-controlled modifications have advanced our understanding of the mechanisms of genome stability, using the modulation of DNA repair genes as examples.
The review discusses various CRISPR systems and their broad utility in genome manipulation, including how CRISPR-controlled modification of DNA repair genes has advanced understanding of genome stability mechanisms.
In this review, we discuss the various CRISPR systems and their broad utility in genome manipulation. We will explore how CRISPR-controlled modifications have advanced our understanding of the mechanisms of genome stability, using the modulation of DNA repair genes as examples.
The review discusses various CRISPR systems and their broad utility in genome manipulation, including how CRISPR-controlled modification of DNA repair genes has advanced understanding of genome stability mechanisms.
In this review, we discuss the various CRISPR systems and their broad utility in genome manipulation. We will explore how CRISPR-controlled modifications have advanced our understanding of the mechanisms of genome stability, using the modulation of DNA repair genes as examples.
Approval Evidence
7 sources17 linked approval claimsfirst-pass slug crispr
Viral (lentiviral, gamma-retroviral) and non-viral (CRISPR, transposons, mRNA electroporation) delivery systems are compared for efficiency, safety, and scalability, with CRISPR enabling multiplex edits for improved specificity.
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CRISPR is a commonly used functional genomics tool that has been successfully used to both enhance our understanding of mechanisms of resistance and to discover potential genetic edits to improve ACT.
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Over the last decade, CRISPR has revolutionized drug development... However, the application of CRISPR systems is associated with unintended off-target and on-target alterations...
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Gene therapies such as antisense oligonucleotides (ASOs), RNA interference (RNAi), CRISPR, and virus-based delivery systems have played crucial roles in discovering and validating new pain targets.
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CRISPR (clustered regularly interspaced short palindromic repeats) is becoming one of the most functional and widely used tools for RNA and DNA manipulation in multiple organisms.
Source:
The discovery of clustered regularly interspaced short palindromic repeat sequences (CRISPR) in Escherichia coli dates to 1987.
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OpenAlex concepts associated with this review include "CRISPR"; the review title explicitly emphasizes genetic screening approaches, and the supplied web research summary highlights CRISPR-linked screening resources as relevant to downstream extraction from this review.
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capabilitysupports
CRISPR has been used to understand mechanisms of resistance and to discover potential genetic edits to improve adoptive cell therapy.
CRISPR is a commonly used functional genomics tool that has been successfully used to both enhance our understanding of mechanisms of resistance and to discover potential genetic edits to improve ACT.
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engineering capabilitysupports
CRISPR enables multiplex edits for improved specificity in CAR-T engineering.
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strategysupports
Complementary functional genomics approaches can be combined and improved to identify translatable genetic editing strategies through studies that accurately recapitulate disease-specific challenges.
Complementary approaches can be combined and improved on to identify translatable genetic editing strategies through studies that accurately recapitulate disease-specific challenges.
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broad capabilitysupports
CRISPR has transformed drug development by enabling potential treatments for genetic diseases that previously lacked therapies.
Over the last decade, CRISPR has revolutionized drug development due to its potential to cure genetic diseases that currently do not have any treatment.
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preclinical relevancesupports
Off-target assessment methods and safety-improvement strategies are relevant to pre-clinical risk assessment of CRISPR therapeutics within the current regulatory context.
Finally, we discuss their relevance and application for the pre-clinical risk assessment of CRISPR therapeutics within the current regulatory context.
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review summarysupports
Gene-therapy modalities including ASOs, RNAi, CRISPR, and virus-based delivery systems have played crucial roles in discovering and validating new pain targets.
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risk summarysupports
Application of CRISPR systems is associated with unintended off-target and on-target alterations, including small indels and structural variations such as translocations, inversions, and large deletions, creating safety risk for patients.
However, the application of CRISPR systems is associated with unintended off-target and on-target alterations (including small indels, and structural variations such as translocations, inversions and large deletions), which are a source of risk for patients and a vital concern for the development of safe therapies.
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scope of reviewsupports
The review covers ASOs, siRNAs, optogenetics, chemogenetics, CRISPR, and their delivery methods targeting primary sensory neurons and non-neuronal cells including glia and chondrocytes.
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translational landscapesupports
Although gene therapy-based clinical trials have increased, trials focused on pain as the primary outcome remain uncommon.
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application areasupports
The review emphasizes CRISPR applications in virus diagnosis and in identifying significant genes involved in virus-host interactions.
Furthermore, we have emphasized the application of CRISPR technology in virus diagnosis and in finding significant genes involved in virus-host interactions.
Source:
application areasupports
The review states that CRISPR enables targeted manipulation of viral genomes, including examples involving SARS-CoV-2, HIV-1, and vaccinia virus.
This approach has provided an unprecedented opportunity for creating simple, inexpensive, specific, targeted, accurate, and practical manipulations of viruses, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), human immunodeficiency virus-1 (HIV-1), and vaccinia virus.
Source:
broad application scopesupports
The review describes CRISPR as a widely used tool for RNA and DNA manipulation in multiple organisms.
CRISPR (clustered regularly interspaced short palindromic repeats) is becoming one of the most functional and widely used tools for RNA and DNA manipulation in multiple organisms.
Source:
design requirementsupports
The review states that a valid and scientifically designed CRISPR system is critical for more effective and accurate viral changes.
Nevertheless, a valid and scientifically designed CRISPR system is critical to make more effective and accurate changes in viruses.
Source:
diagnostic applicationsupports
The review states that CRISPR can be used for effective and precise diagnosis of viral infections.
Furthermore, this method can be used to make an effective and precise diagnosis of viral infections.
Source:
historical functionsupports
CRISPR and Cas proteins were identified as part of a microbial adaptive immune system that targets phage DNA to fight bacteriophage reinfection.
CRISPR and the CRISPR-associated (Cas) proteins were identified as part of the microbial adaptive immune system, by targeting phage DNA, to fight bacteriophage reinfection.
Source:
review scope statementsupports
The review discusses various CRISPR systems and their broad utility in genome manipulation, including how CRISPR-controlled modification of DNA repair genes has advanced understanding of genome stability mechanisms.
In this review, we discuss the various CRISPR systems and their broad utility in genome manipulation. We will explore how CRISPR-controlled modifications have advanced our understanding of the mechanisms of genome stability, using the modulation of DNA repair genes as examples.
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topic associationsupports
CRISPR and RNA interference are relevant screening modalities associated with this review's topic of genetic screening approaches.
OpenAlex concepts include "RNA interference" and "CRISPR"; title includes "genetic screening approaches".
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Comparisons
Source-backed strengths
The supplied review describes CRISPR as one of the most functional and widely used tools for RNA and DNA manipulation in multiple organisms. It is presented as enabling targeted viral genome manipulation and effective, precise diagnosis of viral infections.
Source:
The ease of design, low cytotoxicity, and increased efficiency have made CRISPR/Cas9 and its related systems the designer nucleases of choice for many.
Source:
While these approaches provided sequence-specific gene-editing capacity, the laborious process of designing and synthesizing recombinant nucleases to recognize a specific target sequence, combined with limited target choices and poor editing efficiency, ultimately minimized the broad utility of these systems.
Ranked Citations
- 1.
- 2.