Source 1review2015Genomics Proteomics & Bioinformatics
Toolkit/CRISPR/Cas
CRISPR/Cas
Also known as: clustered regularly-interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas), CRISPR
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
CRISPR/Cas is a DNA manipulation and genome editing technology derived from a prokaryotic immune system. It recognizes and destroys invading genetic elements and has progressed from bench research toward clinical practice.
Usefulness & Problems
Why this is useful
The cited literature describes CRISPR/Cas as a molecular tool that has revolutionized genome editing. It is useful because it has undergone technical improvements and has been applied widely across many model systems.
Source:
These approaches can be used to develop potential therapeutic strategies to effectively treat heritable diseases.
Problem solved
CRISPR/Cas helps solve the problem of manipulating DNA in a targeted manner for genome editing. The evidence also indicates that it derives from a natural system for recognizing and destroying invading genetic elements.
Source:
These approaches can be used to develop potential therapeutic strategies to effectively treat heritable diseases.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
genome editingnucleic acid destructionsequence-specific recognition of invading genetic elementsTechniques
Directed EvolutionTarget processes
editingtranscriptionImplementation Constraints
The available evidence identifies CRISPR/Cas as originating from a prokaryotic immune system, but it does not report construct architecture or required components in detail. No specific information is provided on cofactors, expression systems, delivery strategies, or design rules.
The supplied evidence does not provide specific performance metrics, target scope, editing precision, or comparative limitations relative to other genome editing systems. It also does not specify particular Cas proteins, guide formats, delivery methods, or experimental contexts.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
ZFNs, TALENs, and CRISPR/Cas are molecular tools for DNA manipulation that have revolutionized genome editing.
Development of molecular tools for DNA manipulation, such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly-interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas), has revolutionized genome editing.
ZFNs, TALENs, and CRISPR/Cas are molecular tools for DNA manipulation that have revolutionized genome editing.
Development of molecular tools for DNA manipulation, such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly-interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas), has revolutionized genome editing.
ZFNs, TALENs, and CRISPR/Cas are molecular tools for DNA manipulation that have revolutionized genome editing.
Development of molecular tools for DNA manipulation, such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly-interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas), has revolutionized genome editing.
ZFNs, TALENs, and CRISPR/Cas are molecular tools for DNA manipulation that have revolutionized genome editing.
Development of molecular tools for DNA manipulation, such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly-interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas), has revolutionized genome editing.
ZFNs, TALENs, and CRISPR/Cas are molecular tools for DNA manipulation that have revolutionized genome editing.
Development of molecular tools for DNA manipulation, such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly-interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas), has revolutionized genome editing.
ZFNs, TALENs, and CRISPR/Cas are molecular tools for DNA manipulation that have revolutionized genome editing.
Development of molecular tools for DNA manipulation, such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly-interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas), has revolutionized genome editing.
ZFNs, TALENs, and CRISPR/Cas are molecular tools for DNA manipulation that have revolutionized genome editing.
Development of molecular tools for DNA manipulation, such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly-interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas), has revolutionized genome editing.
CRISPR/Cas technology has seen substantial recent progress, including technical improvements and wide application in many model systems.
In the last few years, substantial progress has been made in CRISPR/Cas technology, including technical improvements and wide application in many model systems.
These genome editing approaches can be used to develop potential therapeutic strategies to treat heritable diseases.
These approaches can be used to develop potential therapeutic strategies to effectively treat heritable diseases.
These genome editing approaches can be used to develop potential therapeutic strategies to treat heritable diseases.
These approaches can be used to develop potential therapeutic strategies to effectively treat heritable diseases.
These genome editing approaches can be used to develop potential therapeutic strategies to treat heritable diseases.
These approaches can be used to develop potential therapeutic strategies to effectively treat heritable diseases.
These genome editing approaches can be used to develop potential therapeutic strategies to treat heritable diseases.
These approaches can be used to develop potential therapeutic strategies to effectively treat heritable diseases.
These genome editing approaches can be used to develop potential therapeutic strategies to treat heritable diseases.
These approaches can be used to develop potential therapeutic strategies to effectively treat heritable diseases.
These genome editing approaches can be used to develop potential therapeutic strategies to treat heritable diseases.
These approaches can be used to develop potential therapeutic strategies to effectively treat heritable diseases.
These genome editing approaches can be used to develop potential therapeutic strategies to treat heritable diseases.
These approaches can be used to develop potential therapeutic strategies to effectively treat heritable diseases.
Approval Evidence
3 sources9 linked approval claimsfirst-pass slug crispr-cas
Among these tools, CRISPR/Cas has stood out for its versatility and its ability to achieve precision levels ranging from 50% to 90%, compared to the 10-40% obtained with earlier techniques, thereby enabling remarkable improvements in bacterial productivity.
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CRISPR-Cas has significantly progressed as alteration tool from bench to clinical practices. CRISPR-Cas is an immune system discovered in prokaryotes that enables organism to recognize and destroy any invading genetic elements.
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Development of molecular tools for DNA manipulation, such as ... the clustered regularly-interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas)
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comparative performancesupports
The review states that CRISPR/Cas can achieve bacterial engineering precision levels of 50% to 90%, compared with 10% to 40% for earlier techniques.
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impact on productivitysupports
The review links CRISPR/Cas-enabled precision bacterial genome engineering to remarkable improvements in bacterial productivity.
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maturity limitationsupports
Despite its advantages, CRISPR/Cas still requires continuous refinement and has not yet reached full maturity for bacterial genome engineering.
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application scopesupports
CRISPR-Cas has progressed as a genome alteration tool from bench to clinical practice.
CRISPR-Cas has significantly progressed as alteration tool from bench to clinical practices.
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application scopesupports
CRISPR functional properties have enabled applications in diagnostics, agriculture, and therapeutics.
This functional property of CRISPR has opened up plethora of applications across different disciplines such as diagnostics, agriculture and therapeutics.
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mechanismsupports
CRISPR-Cas is a prokaryotic immune system that recognizes and destroys invading genetic elements.
CRISPR-Cas is an immune system discovered in prokaryotes that enables organism to recognize and destroy any invading genetic elements.
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broad capability summarysupports
ZFNs, TALENs, and CRISPR/Cas are molecular tools for DNA manipulation that have revolutionized genome editing.
Development of molecular tools for DNA manipulation, such as zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly-interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas), has revolutionized genome editing.
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progress summarysupports
CRISPR/Cas technology has seen substantial recent progress, including technical improvements and wide application in many model systems.
In the last few years, substantial progress has been made in CRISPR/Cas technology, including technical improvements and wide application in many model systems.
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therapeutic potential summarysupports
These genome editing approaches can be used to develop potential therapeutic strategies to treat heritable diseases.
These approaches can be used to develop potential therapeutic strategies to effectively treat heritable diseases.
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Comparisons
Source-backed strengths
According to the cited review, CRISPR/Cas has substantially progressed as a genetic alteration tool and has seen wide application in many model systems. The literature further characterizes it as part of a set of tools that revolutionized genome editing.
Ranked Citations
- 1.