Source 1primary paper2025
Toolkit/CRISPR-Cas genome editors directly modulated by temperature
CRISPR-Cas genome editors directly modulated by temperature
Also known as: thermo-modulated CRISPR-Cas genome editors
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Thermo-modulated CRISPR-Cas genome editors are engineered CRISPR-Cas constructs in mammalian systems whose genome-editing activity is directly modulated by subtle temperature changes within the physiological range. The reported work describes these as the first CRISPR-Cas genome editors with direct temperature responsiveness.
Usefulness & Problems
Why this is useful
These constructs provide a route to regulate CRISPR-Cas editing activity using small temperature shifts rather than only chemical or optical inputs. The source frames this as part of a generalizable strategy for building thermosensitive allosteric proteins through insertion of optimized Avena sativa LOV2 domain variants.
Problem solved
The tool addresses the problem of directly coupling CRISPR-Cas genome editor activity to temperature changes in mammalian systems. More broadly, it targets the engineering challenge of creating thermosensitive allosteric control over protein function.
Problem links
Need controllable genome or transcript editing
DerivedThermo-modulated CRISPR-Cas genome editors are engineered CRISPR-Cas constructs in mammalian systems whose genome-editing activity is directly modulated by subtle temperature changes within the physiological range. The reported work describes these as the first CRISPR-Cas genome editors with direct temperature responsiveness.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
editingImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: regulatorswitch architecture: uncaging
The reported engineering strategy uses insertion of optimized Avena sativa LOV2 domain variants to create thermosensitive allosteric proteins, indicating domain insertion as a core construct design principle. A chemoreceptor domain is also cited as an alternative thermosensing module, but the provided evidence does not include construct architecture, linker design, delivery method, or expression details for the CRISPR-Cas editors.
The supplied evidence does not specify which Cas effector, editing modality, temperature setpoints, dynamic range, reversibility, or editing outcomes were validated. Independent replication and breadth across multiple CRISPR systems or biological contexts are not documented in the provided material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The authors engineered CRISPR-Cas genome editors in mammalian systems that are directly modulated by subtle temperature changes within the physiological range. Derived from claim c3. Quoted text: Extending this strategy to mammalian systems, we engineered the first CRISPR-Cas genome editors directly modulated by subtle temperature changes within the physiological range.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The authors engineered CRISPR-Cas genome editors in mammalian systems that are directly modulated by subtle temperature changes within the physiological range. Derived from claim c3. Quoted text: Extending this strategy to mammalian systems, we engineered the first CRISPR-Cas genome editors directly modulated by subtle temperature changes within the physiological range.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The authors engineered CRISPR-Cas genome editors in mammalian systems that are directly modulated by subtle temperature changes within the physiological range. Derived from claim c3. Quoted text: Extending this strategy to mammalian systems, we engineered the first CRISPR-Cas genome editors directly modulated by subtle temperature changes within the physiological range.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The authors engineered CRISPR-Cas genome editors in mammalian systems that are directly modulated by subtle temperature changes within the physiological range. Derived from claim c3. Quoted text: Extending this strategy to mammalian systems, we engineered the first CRISPR-Cas genome editors directly modulated by subtle temperature changes within the physiological range.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The authors engineered CRISPR-Cas genome editors in mammalian systems that are directly modulated by subtle temperature changes within the physiological range. Derived from claim c3. Quoted text: Extending this strategy to mammalian systems, we engineered the first CRISPR-Cas genome editors directly modulated by subtle temperature changes within the physiological range.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The authors engineered CRISPR-Cas genome editors in mammalian systems that are directly modulated by subtle temperature changes within the physiological range. Derived from claim c3. Quoted text: Extending this strategy to mammalian systems, we engineered the first CRISPR-Cas genome editors directly modulated by subtle temperature changes within the physiological range.
Source:
successMammalian Cell Lineapplication demo
Inferred from claim c3 during normalization. The authors engineered CRISPR-Cas genome editors in mammalian systems that are directly modulated by subtle temperature changes within the physiological range. Derived from claim c3. Quoted text: Extending this strategy to mammalian systems, we engineered the first CRISPR-Cas genome editors directly modulated by subtle temperature changes within the physiological range.
Source:
Supporting Sources
Ranked Claims
The paper presents a generalizable strategy for engineering thermosensitive allosteric proteins by inserting optimized Avena sativa LOV2 domain variants.
we present a generalizable strategy for engineering thermosensitive allosteric proteins through the insertion of optimized Avena sativa LOV2 domain variants
The paper presents a generalizable strategy for engineering thermosensitive allosteric proteins by inserting optimized Avena sativa LOV2 domain variants.
we present a generalizable strategy for engineering thermosensitive allosteric proteins through the insertion of optimized Avena sativa LOV2 domain variants
The paper presents a generalizable strategy for engineering thermosensitive allosteric proteins by inserting optimized Avena sativa LOV2 domain variants.
we present a generalizable strategy for engineering thermosensitive allosteric proteins through the insertion of optimized Avena sativa LOV2 domain variants
The paper presents a generalizable strategy for engineering thermosensitive allosteric proteins by inserting optimized Avena sativa LOV2 domain variants.
we present a generalizable strategy for engineering thermosensitive allosteric proteins through the insertion of optimized Avena sativa LOV2 domain variants
The paper presents a generalizable strategy for engineering thermosensitive allosteric proteins by inserting optimized Avena sativa LOV2 domain variants.
we present a generalizable strategy for engineering thermosensitive allosteric proteins through the insertion of optimized Avena sativa LOV2 domain variants
The paper presents a generalizable strategy for engineering thermosensitive allosteric proteins by inserting optimized Avena sativa LOV2 domain variants.
we present a generalizable strategy for engineering thermosensitive allosteric proteins through the insertion of optimized Avena sativa LOV2 domain variants
The paper presents a generalizable strategy for engineering thermosensitive allosteric proteins by inserting optimized Avena sativa LOV2 domain variants.
we present a generalizable strategy for engineering thermosensitive allosteric proteins through the insertion of optimized Avena sativa LOV2 domain variants
The authors engineered CRISPR-Cas genome editors in mammalian systems that are directly modulated by subtle temperature changes within the physiological range.
Extending this strategy to mammalian systems, we engineered the first CRISPR-Cas genome editors directly modulated by subtle temperature changes within the physiological range.
The authors engineered CRISPR-Cas genome editors in mammalian systems that are directly modulated by subtle temperature changes within the physiological range.
Extending this strategy to mammalian systems, we engineered the first CRISPR-Cas genome editors directly modulated by subtle temperature changes within the physiological range.
The authors engineered CRISPR-Cas genome editors in mammalian systems that are directly modulated by subtle temperature changes within the physiological range.
Extending this strategy to mammalian systems, we engineered the first CRISPR-Cas genome editors directly modulated by subtle temperature changes within the physiological range.
The authors engineered CRISPR-Cas genome editors in mammalian systems that are directly modulated by subtle temperature changes within the physiological range.
Extending this strategy to mammalian systems, we engineered the first CRISPR-Cas genome editors directly modulated by subtle temperature changes within the physiological range.
The authors engineered CRISPR-Cas genome editors in mammalian systems that are directly modulated by subtle temperature changes within the physiological range.
Extending this strategy to mammalian systems, we engineered the first CRISPR-Cas genome editors directly modulated by subtle temperature changes within the physiological range.
The authors engineered CRISPR-Cas genome editors in mammalian systems that are directly modulated by subtle temperature changes within the physiological range.
Extending this strategy to mammalian systems, we engineered the first CRISPR-Cas genome editors directly modulated by subtle temperature changes within the physiological range.
The authors engineered CRISPR-Cas genome editors in mammalian systems that are directly modulated by subtle temperature changes within the physiological range.
Extending this strategy to mammalian systems, we engineered the first CRISPR-Cas genome editors directly modulated by subtle temperature changes within the physiological range.
A chemoreceptor domain can serve as an alternative thermosensing module, suggesting thermo-sensitivity may be widespread in receptor domains.
we showcase the incorporation of a chemoreceptor domain as an alternative thermosensing module, suggesting thermo-sensitivity to be a widespread feature in receptor domains
This work expands the thermogenetics toolkit and provides a blueprint for temperature-dependent control of virtually any protein of interest.
This work expands the toolkit of thermogenetics, providing a blueprint for temperature-dependent control of virtually any protein of interest.
This work expands the thermogenetics toolkit and provides a blueprint for temperature-dependent control of virtually any protein of interest.
This work expands the toolkit of thermogenetics, providing a blueprint for temperature-dependent control of virtually any protein of interest.
This work expands the thermogenetics toolkit and provides a blueprint for temperature-dependent control of virtually any protein of interest.
This work expands the toolkit of thermogenetics, providing a blueprint for temperature-dependent control of virtually any protein of interest.
This work expands the thermogenetics toolkit and provides a blueprint for temperature-dependent control of virtually any protein of interest.
This work expands the toolkit of thermogenetics, providing a blueprint for temperature-dependent control of virtually any protein of interest.
This work expands the thermogenetics toolkit and provides a blueprint for temperature-dependent control of virtually any protein of interest.
This work expands the toolkit of thermogenetics, providing a blueprint for temperature-dependent control of virtually any protein of interest.
This work expands the thermogenetics toolkit and provides a blueprint for temperature-dependent control of virtually any protein of interest.
This work expands the toolkit of thermogenetics, providing a blueprint for temperature-dependent control of virtually any protein of interest.
This work expands the thermogenetics toolkit and provides a blueprint for temperature-dependent control of virtually any protein of interest.
This work expands the toolkit of thermogenetics, providing a blueprint for temperature-dependent control of virtually any protein of interest.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug crispr-cas-genome-editors-directly-modulated-by-temperature
we engineered the first CRISPR-Cas genome editors directly modulated by subtle temperature changes within the physiological range
Source:
first of kindsupports
The authors engineered CRISPR-Cas genome editors in mammalian systems that are directly modulated by subtle temperature changes within the physiological range.
Extending this strategy to mammalian systems, we engineered the first CRISPR-Cas genome editors directly modulated by subtle temperature changes within the physiological range.
Source:
scope statementsupports
This work expands the thermogenetics toolkit and provides a blueprint for temperature-dependent control of virtually any protein of interest.
This work expands the toolkit of thermogenetics, providing a blueprint for temperature-dependent control of virtually any protein of interest.
Source:
Comparisons
Source-backed strengths
The source claims direct modulation of CRISPR-Cas genome editors by subtle temperature changes within the physiological range, which is a stringent operating regime for mammalian applications. The associated engineering framework is described as generalizable, and an alternative chemoreceptor thermosensing module is reported, supporting modularity of the design concept.
Source:
we present a generalizable strategy for engineering thermosensitive allosteric proteins through the insertion of optimized Avena sativa LOV2 domain variants
Compared with microfluidic organ-on-chip platforms
CRISPR-Cas genome editors directly modulated by temperature and microfluidic organ-on-chip platforms address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing
Strengths here: looks easier to implement in practice.
Compared with synthetic promoters
CRISPR-Cas genome editors directly modulated by temperature and synthetic promoters address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Compared with Z7-E78-ABE
CRISPR-Cas genome editors directly modulated by temperature and Z7-E78-ABE address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing
Ranked Citations
- 1.