Source 1review2021Microorganisms
Toolkit/CRISPR-Cas-mediated genome editing
CRISPR-Cas-mediated genome editing
Also known as: CRISPR-Cas
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
CRISPR-Cas-mediated genome editing is a programmable genome-editing approach discussed here in the context of bacterial systems. The cited review summarizes the main approaches for bacterial CRISPR-Cas editing and the difficulties associated with applying these systems in bacteria.
Usefulness & Problems
Why this is useful
This approach is useful because CRISPR-Cas technologies are characterized in the source as simple and programmable, properties that drove their broad impact in eukaryotic genome editing. In bacteria, the review positions CRISPR-Cas as a genome-editing strategy to be considered alongside more laborious multi-step methods such as suicide plasmids.
Problem solved
CRISPR-Cas-mediated genome editing addresses the need for programmable genome modification in bacterial systems. The review specifically frames the problem as how to perform bacterial genome editing despite difficulties that have limited adoption of CRISPR-Cas in bacteria relative to eukaryotes.
Published Workflows
Objective: Accelerate the development of climate-resilient Capsicum cultivars with optimized metabolic traits.
Why it works: The abstract argues that combining molecular insight from transcriptional, metabolic, and epigenetic analysis with precision phenotyping and genome editing should enable targeted reprogramming of regulatory loci that control adaptive responses and metabolic outputs.
transcription factor modulationosmotic adjustmentreactive oxygen species detoxificationhormonal crosstalksecondary metabolite biosynthesismulti-omics-guided gene discoveryprecision phenotypingCRISPR/Cas-mediated genome editingnext-generation genome editing
Stages
- 1.multi-omics-guided gene discovery(in_silico_filter)
The abstract positions multi-omics-guided gene discovery as the upstream step that identifies targets for subsequent genome editing.
Selection: Identification of key regulatory loci and stress-resilience frameworks from integrated transcriptional, metabolic, and epigenetic information.
- 2.precision phenotyping(functional_characterization)
The abstract includes precision phenotyping as a core component of the proposed framework linking molecular targets to cultivar-level performance.
Selection: Phenotypic assessment within the proposed framework for climate resilience and optimized metabolic traits.
- 3.next-generation genome editing(confirmatory_validation)
The abstract presents genome editing as the intervention step that operationalizes targets identified through transcription factor analysis and multi-omics-guided discovery.
Selection: Precise reprogramming of key regulatory loci to enhance adaptive responses.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Techniques
Directed EvolutionTarget processes
editingtranscriptionImplementation Constraints
The review includes fluorescent-protein-based methods for evaluating efficacy of CRISPR-based genome-editing systems in bacteria. No construct architecture, delivery modality, host range, or cofactor requirements are provided in the supplied evidence.
The cited evidence states that CRISPR-Cas technologies have not been as widely favored for bacterial genome editing as they have been in eukaryotes. The available evidence here does not specify particular Cas proteins, editing outcomes, efficiencies, or bacterial species, so performance constraints cannot be detailed further from this source set.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2primary paper2025MED
Ranked Claims
CRISPR/Cas-mediated genome editing, stress-inducible promoter engineering, and synthetic transcriptional circuits are promising strategies for fine-tuning HSF expression and enhancing multi-stress resilience in crops.
We also discuss biotechnological strategies such as CRISPR/Cas-mediated genome editing, stress-inducible promoter engineering, and synthetic transcriptional circuits that offer promising avenues for fine-tuning HSF expression and enhancing multi-stress resilience in crops.
Although CRISPR-Cas technologies revolutionized genome editing in eukaryotes because of simplicity and programmability, they have not been as widely favored for bacterial genome editing.
The discovery and applications of clustered regularly interspaced short palindromic repeats (CRISPR)-Cas based technologies have revolutionized genome editing in eukaryotic organisms due to its simplicity and programmability. Nevertheless, this system has not been as widely favored for bacterial genome editing.
Section: abstract
The review addresses fluorescent-protein-based methods for evaluating the efficacy of CRISPR-based genome-editing systems in bacteria.
Finally, we also address fluorescent-protein-based methods to evaluate the efficacy of CRISPR-based systems for genome editing in bacteria.
Section: abstract
The review addresses fluorescent-protein-based methods for evaluating the efficacy of CRISPR-based genome-editing systems in bacteria.
Finally, we also address fluorescent-protein-based methods to evaluate the efficacy of CRISPR-based systems for genome editing in bacteria.
Section: abstract
The review addresses fluorescent-protein-based methods for evaluating the efficacy of CRISPR-based genome-editing systems in bacteria.
Finally, we also address fluorescent-protein-based methods to evaluate the efficacy of CRISPR-based systems for genome editing in bacteria.
Section: abstract
The review addresses fluorescent-protein-based methods for evaluating the efficacy of CRISPR-based genome-editing systems in bacteria.
Finally, we also address fluorescent-protein-based methods to evaluate the efficacy of CRISPR-based systems for genome editing in bacteria.
Section: abstract
The review addresses fluorescent-protein-based methods for evaluating the efficacy of CRISPR-based genome-editing systems in bacteria.
Finally, we also address fluorescent-protein-based methods to evaluate the efficacy of CRISPR-based systems for genome editing in bacteria.
Section: abstract
The review addresses fluorescent-protein-based methods for evaluating the efficacy of CRISPR-based genome-editing systems in bacteria.
Finally, we also address fluorescent-protein-based methods to evaluate the efficacy of CRISPR-based systems for genome editing in bacteria.
Section: abstract
The review addresses fluorescent-protein-based methods for evaluating the efficacy of CRISPR-based genome-editing systems in bacteria.
Finally, we also address fluorescent-protein-based methods to evaluate the efficacy of CRISPR-based systems for genome editing in bacteria.
Section: abstract
Bacterial genome editing includes laborious and multi-step methods such as suicide plasmids.
Genome editing in bacteria encompasses a wide array of laborious and multi-step methods such as suicide plasmids.
Section: abstract
Bacterial genome editing includes laborious and multi-step methods such as suicide plasmids.
Genome editing in bacteria encompasses a wide array of laborious and multi-step methods such as suicide plasmids.
Section: abstract
Bacterial genome editing includes laborious and multi-step methods such as suicide plasmids.
Genome editing in bacteria encompasses a wide array of laborious and multi-step methods such as suicide plasmids.
Section: abstract
Bacterial genome editing includes laborious and multi-step methods such as suicide plasmids.
Genome editing in bacteria encompasses a wide array of laborious and multi-step methods such as suicide plasmids.
Section: abstract
Bacterial genome editing includes laborious and multi-step methods such as suicide plasmids.
Genome editing in bacteria encompasses a wide array of laborious and multi-step methods such as suicide plasmids.
Section: abstract
Bacterial genome editing includes laborious and multi-step methods such as suicide plasmids.
Genome editing in bacteria encompasses a wide array of laborious and multi-step methods such as suicide plasmids.
Section: abstract
Bacterial genome editing includes laborious and multi-step methods such as suicide plasmids.
Genome editing in bacteria encompasses a wide array of laborious and multi-step methods such as suicide plasmids.
Section: abstract
CRISPR-Cas still holds promise as a generalized genome-editing tool in bacteria and is undergoing further optimization for expanded application in these organisms.
CRISPR-Cas still holds promise as a generalized genome-editing tool in bacteria and is developing further optimization for an expanded application in these organisms.
Section: abstract
The review summarizes main approaches and difficulties associated with CRISPR-Cas-mediated genome editing in bacteria and presents alternatives intended to circumvent these issues.
In this review, we summarize the main approaches and difficulties associated with CRISPR-Cas-mediated genome editing in bacteria and present some alternatives to circumvent these issues, including CRISPR nickases, Cas12a, base editors, CRISPR-associated transposases, prime-editing, endogenous CRISPR systems, and the use of pre-made ribonucleoprotein complexes of Cas proteins and guide RNAs.
Section: abstract
The review summarizes main approaches and difficulties associated with CRISPR-Cas-mediated genome editing in bacteria and presents alternatives intended to circumvent these issues.
In this review, we summarize the main approaches and difficulties associated with CRISPR-Cas-mediated genome editing in bacteria and present some alternatives to circumvent these issues, including CRISPR nickases, Cas12a, base editors, CRISPR-associated transposases, prime-editing, endogenous CRISPR systems, and the use of pre-made ribonucleoprotein complexes of Cas proteins and guide RNAs.
Section: abstract
The review summarizes main approaches and difficulties associated with CRISPR-Cas-mediated genome editing in bacteria and presents alternatives intended to circumvent these issues.
In this review, we summarize the main approaches and difficulties associated with CRISPR-Cas-mediated genome editing in bacteria and present some alternatives to circumvent these issues, including CRISPR nickases, Cas12a, base editors, CRISPR-associated transposases, prime-editing, endogenous CRISPR systems, and the use of pre-made ribonucleoprotein complexes of Cas proteins and guide RNAs.
Section: abstract
The review summarizes main approaches and difficulties associated with CRISPR-Cas-mediated genome editing in bacteria and presents alternatives intended to circumvent these issues.
In this review, we summarize the main approaches and difficulties associated with CRISPR-Cas-mediated genome editing in bacteria and present some alternatives to circumvent these issues, including CRISPR nickases, Cas12a, base editors, CRISPR-associated transposases, prime-editing, endogenous CRISPR systems, and the use of pre-made ribonucleoprotein complexes of Cas proteins and guide RNAs.
Section: abstract
The review summarizes main approaches and difficulties associated with CRISPR-Cas-mediated genome editing in bacteria and presents alternatives intended to circumvent these issues.
In this review, we summarize the main approaches and difficulties associated with CRISPR-Cas-mediated genome editing in bacteria and present some alternatives to circumvent these issues, including CRISPR nickases, Cas12a, base editors, CRISPR-associated transposases, prime-editing, endogenous CRISPR systems, and the use of pre-made ribonucleoprotein complexes of Cas proteins and guide RNAs.
Section: abstract
The review summarizes main approaches and difficulties associated with CRISPR-Cas-mediated genome editing in bacteria and presents alternatives intended to circumvent these issues.
In this review, we summarize the main approaches and difficulties associated with CRISPR-Cas-mediated genome editing in bacteria and present some alternatives to circumvent these issues, including CRISPR nickases, Cas12a, base editors, CRISPR-associated transposases, prime-editing, endogenous CRISPR systems, and the use of pre-made ribonucleoprotein complexes of Cas proteins and guide RNAs.
Section: abstract
The review summarizes main approaches and difficulties associated with CRISPR-Cas-mediated genome editing in bacteria and presents alternatives intended to circumvent these issues.
In this review, we summarize the main approaches and difficulties associated with CRISPR-Cas-mediated genome editing in bacteria and present some alternatives to circumvent these issues, including CRISPR nickases, Cas12a, base editors, CRISPR-associated transposases, prime-editing, endogenous CRISPR systems, and the use of pre-made ribonucleoprotein complexes of Cas proteins and guide RNAs.
Section: abstract
Approval Evidence
3 sources6 linked approval claimsfirst-pass slug crispr-cas-mediated-genome-editing
We also discuss biotechnological strategies such as CRISPR/Cas-mediated genome editing, stress-inducible promoter engineering, and synthetic transcriptional circuits that offer promising avenues for fine-tuning HSF expression and enhancing multi-stress resilience in crops.
Source:
We further highlight how multi-omics-guided gene discovery, when paired with CRISPR/Cas-mediated genome editing, enables precise reprogramming of key regulatory loci to enhance adaptive responses.
Source:
we summarize the main approaches and difficulties associated with CRISPR-Cas-mediated genome editing in bacteria
Source:
engineering capabilitysupports
Multi-omics-guided gene discovery paired with CRISPR/Cas-mediated genome editing enables precise reprogramming of key regulatory loci to enhance adaptive responses.
We further highlight how multi-omics-guided gene discovery, when paired with CRISPR/Cas-mediated genome editing, enables precise reprogramming of key regulatory loci to enhance adaptive responses.
Source:
engineering promisesupports
CRISPR/Cas-mediated genome editing, stress-inducible promoter engineering, and synthetic transcriptional circuits are promising strategies for fine-tuning HSF expression and enhancing multi-stress resilience in crops.
We also discuss biotechnological strategies such as CRISPR/Cas-mediated genome editing, stress-inducible promoter engineering, and synthetic transcriptional circuits that offer promising avenues for fine-tuning HSF expression and enhancing multi-stress resilience in crops.
Source:
comparative adoptionsupports
Although CRISPR-Cas technologies revolutionized genome editing in eukaryotes because of simplicity and programmability, they have not been as widely favored for bacterial genome editing.
The discovery and applications of clustered regularly interspaced short palindromic repeats (CRISPR)-Cas based technologies have revolutionized genome editing in eukaryotic organisms due to its simplicity and programmability. Nevertheless, this system has not been as widely favored for bacterial genome editing.
Source:
evaluation method scopesupports
The review addresses fluorescent-protein-based methods for evaluating the efficacy of CRISPR-based genome-editing systems in bacteria.
Finally, we also address fluorescent-protein-based methods to evaluate the efficacy of CRISPR-based systems for genome editing in bacteria.
Source:
promise outlooksupports
CRISPR-Cas still holds promise as a generalized genome-editing tool in bacteria and is undergoing further optimization for expanded application in these organisms.
CRISPR-Cas still holds promise as a generalized genome-editing tool in bacteria and is developing further optimization for an expanded application in these organisms.
Source:
review scope summarysupports
The review summarizes main approaches and difficulties associated with CRISPR-Cas-mediated genome editing in bacteria and presents alternatives intended to circumvent these issues.
In this review, we summarize the main approaches and difficulties associated with CRISPR-Cas-mediated genome editing in bacteria and present some alternatives to circumvent these issues, including CRISPR nickases, Cas12a, base editors, CRISPR-associated transposases, prime-editing, endogenous CRISPR systems, and the use of pre-made ribonucleoprotein complexes of Cas proteins and guide RNAs.
Source:
Comparisons
Source-backed strengths
The source attributes simplicity and programmability to CRISPR-Cas technologies. The review also notes fluorescent-protein-based methods for evaluating the efficacy of CRISPR-based genome-editing systems in bacteria, indicating that assay frameworks exist for performance assessment.
Source:
The discovery and applications of clustered regularly interspaced short palindromic repeats (CRISPR)-Cas based technologies have revolutionized genome editing in eukaryotic organisms due to its simplicity and programmability. Nevertheless, this system has not been as widely favored for bacterial genome editing.
Ranked Citations
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- 2.