Source 1review2015International Journal of Molecular Sciences
Toolkit/CRISPR/Cas system
CRISPR/Cas system
Also known as: clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins, CRISPR-associated (Cas) system, CRISPR-Cas, CRISPR/Cas, CRISPR editing system, CRISPR platform
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The CRISPR/Cas system is a multi-component genomic engineering platform composed of clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins. It is described as a versatile and powerful genetic tool for genome manipulation, with reported applicability across essentially any organism and cell type.
Usefulness & Problems
Why this is useful
The system is useful because it has revolutionized traditional gene-editing approaches and offers broad potential for genetic manipulation across many organisms and cell types. The cited review specifically frames it as a platform whose performance can be improved through sgRNA design and modification, Cas variants, anti-CRISPR proteins, and mutant enrichment strategies.
Source:
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
Source:
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
Source:
This technology has advanced our understanding of complex neurologic diseases by enabling the rapid generation of novel, disease-relevant in vitro and transgenic animal models.
Source:
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein (CRISPR-Cas) system has emerged as a powerful genetic tool capable of manipulating the genome of essentially any organism and cell type.
Problem solved
CRISPR/Cas addresses the need for a broadly applicable genomic engineering system for targeted genome manipulation. The supplied evidence also indicates that current work focuses on solving practical performance problems in CRISPR/Cas use, including mutation efficiency, delivery efficiency, and control of adverse effects.
Source:
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
Source:
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
Source:
This technology has advanced our understanding of complex neurologic diseases by enabling the rapid generation of novel, disease-relevant in vitro and transgenic animal models.
Source:
Engineered DNA-binding molecules such as transcription activator-like effector (TAL or TALE) proteins and the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) (CRISPR/Cas) system have been used extensively for genome editing in cells of various types and species.
Published Workflows
Objective: Employ the CRISPR/Cas system to boost stress tolerance and resistance in Brassica species.
Why it works: The abstract states that recent genomic studies on Brassica crops and their pathogens have facilitated deployment of CRISPR/Cas systems in breeding major Brassica crops.
molecular mechanisms underlying crop responses to biotic and abiotic stressesCRISPR/Cas-based gene editingbreeding deployment informed by genomic studies
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
anti-crispr-mediated inhibitionrna-guided genome editingselection/enrichment of edited mutantsTarget processes
editingrecombinationselectiontranscriptionInput: Chemical
Implementation Constraints
The supplied evidence supports practical attention to sgRNA design and modification as an implementation variable for improving CRISPR/Cas-induced mutation efficiency. It also identifies Cas variants, anti-CRISPR proteins, and mutant enrichment as emerging approaches, but does not provide construct architectures, delivery vehicles, cofactors, or expression-system details.
The cited review states that CRISPR/Cas systems have inherent limitations including off-target effects, unsatisfactory delivery efficiency, and unwanted adverse effects. No quantitative performance benchmarks, organism-specific constraints, or head-to-head comparisons are provided in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2review2020Frontiers in Bioengineering and Biotechnology
Source 3review2020eNeuro
Source 4primary paper2025MED
Source 5review2022Frontiers in Cell and Developmental Biology
Source 6primary paper2025MED
Ranked Claims
CRISPR-Cas has expanding applications in genetics, biotechnology, agriculture, and medicine.
with a particular emphasis on the CRISPR-Cas system and its expanding applications in genetics, biotechnology, agriculture, and medicine
The CRISPR-Cas system has emerged as the most extensively employed gene-editing platform because of its simplicity, low cost, and efficiency.
Among the diverse gene-editing platforms, the CRISPR-Cas system has emerged as the most extensively employed, owing to its simplicity, low cost, and efficiency.
The CRISPR/Cas system has significant potential for treating viral infections and is positioned as an effective approach for combating HPV by selectively targeting and editing viral genomes.
The review states that CRISPR/Cas systems have inherent limitations including off-target effects, unsatisfactory delivery efficiency, and unwanted adverse effects.
CRISPR/Cas systems have some inherent limitations, such as off-target effects, unsatisfactory efficiency of delivery, and unwanted adverse effects
The review states that CRISPR/Cas systems have inherent limitations including off-target effects, unsatisfactory delivery efficiency, and unwanted adverse effects.
CRISPR/Cas systems have some inherent limitations, such as off-target effects, unsatisfactory efficiency of delivery, and unwanted adverse effects
The review states that CRISPR/Cas systems have inherent limitations including off-target effects, unsatisfactory delivery efficiency, and unwanted adverse effects.
CRISPR/Cas systems have some inherent limitations, such as off-target effects, unsatisfactory efficiency of delivery, and unwanted adverse effects
The review states that CRISPR/Cas systems have inherent limitations including off-target effects, unsatisfactory delivery efficiency, and unwanted adverse effects.
CRISPR/Cas systems have some inherent limitations, such as off-target effects, unsatisfactory efficiency of delivery, and unwanted adverse effects
The review states that CRISPR/Cas systems have inherent limitations including off-target effects, unsatisfactory delivery efficiency, and unwanted adverse effects.
CRISPR/Cas systems have some inherent limitations, such as off-target effects, unsatisfactory efficiency of delivery, and unwanted adverse effects
The review states that CRISPR/Cas systems have inherent limitations including off-target effects, unsatisfactory delivery efficiency, and unwanted adverse effects.
CRISPR/Cas systems have some inherent limitations, such as off-target effects, unsatisfactory efficiency of delivery, and unwanted adverse effects
The review states that CRISPR/Cas systems have revolutionized traditional gene-editing tools and show broad potential for genetic manipulation across many organisms and cell types.
CRISPR/Cas systems have revolutionized traditional gene-editing tools ... have displayed tremendous potential for genetic manipulation in almost any organism and cell type.
The review states that CRISPR/Cas systems have revolutionized traditional gene-editing tools and show broad potential for genetic manipulation across many organisms and cell types.
CRISPR/Cas systems have revolutionized traditional gene-editing tools ... have displayed tremendous potential for genetic manipulation in almost any organism and cell type.
The review states that CRISPR/Cas systems have revolutionized traditional gene-editing tools and show broad potential for genetic manipulation across many organisms and cell types.
CRISPR/Cas systems have revolutionized traditional gene-editing tools ... have displayed tremendous potential for genetic manipulation in almost any organism and cell type.
The review states that CRISPR/Cas systems have revolutionized traditional gene-editing tools and show broad potential for genetic manipulation across many organisms and cell types.
CRISPR/Cas systems have revolutionized traditional gene-editing tools ... have displayed tremendous potential for genetic manipulation in almost any organism and cell type.
The review states that CRISPR/Cas systems have revolutionized traditional gene-editing tools and show broad potential for genetic manipulation across many organisms and cell types.
CRISPR/Cas systems have revolutionized traditional gene-editing tools ... have displayed tremendous potential for genetic manipulation in almost any organism and cell type.
The review states that CRISPR/Cas systems have revolutionized traditional gene-editing tools and show broad potential for genetic manipulation across many organisms and cell types.
CRISPR/Cas systems have revolutionized traditional gene-editing tools ... have displayed tremendous potential for genetic manipulation in almost any organism and cell type.
The review describes choice of delivery system as a strategy for improving the efficiency of CRISPR/Cas-induced mutations.
Strategies for improving the efficiency of CRISPR/Cas-induced mutations, such as ... choice of delivery system ... are comprehensively described in this review.
The review describes choice of delivery system as a strategy for improving the efficiency of CRISPR/Cas-induced mutations.
Strategies for improving the efficiency of CRISPR/Cas-induced mutations, such as ... choice of delivery system ... are comprehensively described in this review.
The review describes choice of delivery system as a strategy for improving the efficiency of CRISPR/Cas-induced mutations.
Strategies for improving the efficiency of CRISPR/Cas-induced mutations, such as ... choice of delivery system ... are comprehensively described in this review.
The review describes choice of delivery system as a strategy for improving the efficiency of CRISPR/Cas-induced mutations.
Strategies for improving the efficiency of CRISPR/Cas-induced mutations, such as ... choice of delivery system ... are comprehensively described in this review.
The review describes choice of delivery system as a strategy for improving the efficiency of CRISPR/Cas-induced mutations.
Strategies for improving the efficiency of CRISPR/Cas-induced mutations, such as ... choice of delivery system ... are comprehensively described in this review.
The review describes choice of delivery system as a strategy for improving the efficiency of CRISPR/Cas-induced mutations.
Strategies for improving the efficiency of CRISPR/Cas-induced mutations, such as ... choice of delivery system ... are comprehensively described in this review.
The review describes choice of delivery system as a strategy for improving the efficiency of CRISPR/Cas-induced mutations.
Strategies for improving the efficiency of CRISPR/Cas-induced mutations, such as ... choice of delivery system ... are comprehensively described in this review.
The review describes improving sgRNA design and modification as a strategy for improving the efficiency of CRISPR/Cas-induced mutations.
Strategies for improving the efficiency of CRISPR/Cas-induced mutations, such as ... improving the design and modification of sgRNA ... are comprehensively described in this review.
The review describes improving sgRNA design and modification as a strategy for improving the efficiency of CRISPR/Cas-induced mutations.
Strategies for improving the efficiency of CRISPR/Cas-induced mutations, such as ... improving the design and modification of sgRNA ... are comprehensively described in this review.
The review describes improving sgRNA design and modification as a strategy for improving the efficiency of CRISPR/Cas-induced mutations.
Strategies for improving the efficiency of CRISPR/Cas-induced mutations, such as ... improving the design and modification of sgRNA ... are comprehensively described in this review.
The review describes improving sgRNA design and modification as a strategy for improving the efficiency of CRISPR/Cas-induced mutations.
Strategies for improving the efficiency of CRISPR/Cas-induced mutations, such as ... improving the design and modification of sgRNA ... are comprehensively described in this review.
The review describes improving sgRNA design and modification as a strategy for improving the efficiency of CRISPR/Cas-induced mutations.
Strategies for improving the efficiency of CRISPR/Cas-induced mutations, such as ... improving the design and modification of sgRNA ... are comprehensively described in this review.
The review describes improving sgRNA design and modification as a strategy for improving the efficiency of CRISPR/Cas-induced mutations.
Strategies for improving the efficiency of CRISPR/Cas-induced mutations, such as ... improving the design and modification of sgRNA ... are comprehensively described in this review.
The review describes improving sgRNA design and modification as a strategy for improving the efficiency of CRISPR/Cas-induced mutations.
Strategies for improving the efficiency of CRISPR/Cas-induced mutations, such as ... improving the design and modification of sgRNA ... are comprehensively described in this review.
The review discusses Cas variants, anti-CRISPR proteins, and mutant enrichment as newly emerging approaches relevant to improving CRISPR/Cas system use.
several newly emerging approaches, including the use of Cas variants, anti-CRISPR proteins, and mutant enrichment, are discussed in detail
The review discusses Cas variants, anti-CRISPR proteins, and mutant enrichment as newly emerging approaches relevant to improving CRISPR/Cas system use.
several newly emerging approaches, including the use of Cas variants, anti-CRISPR proteins, and mutant enrichment, are discussed in detail
The review discusses Cas variants, anti-CRISPR proteins, and mutant enrichment as newly emerging approaches relevant to improving CRISPR/Cas system use.
several newly emerging approaches, including the use of Cas variants, anti-CRISPR proteins, and mutant enrichment, are discussed in detail
The review discusses Cas variants, anti-CRISPR proteins, and mutant enrichment as newly emerging approaches relevant to improving CRISPR/Cas system use.
several newly emerging approaches, including the use of Cas variants, anti-CRISPR proteins, and mutant enrichment, are discussed in detail
The review discusses Cas variants, anti-CRISPR proteins, and mutant enrichment as newly emerging approaches relevant to improving CRISPR/Cas system use.
several newly emerging approaches, including the use of Cas variants, anti-CRISPR proteins, and mutant enrichment, are discussed in detail
The review discusses Cas variants, anti-CRISPR proteins, and mutant enrichment as newly emerging approaches relevant to improving CRISPR/Cas system use.
several newly emerging approaches, including the use of Cas variants, anti-CRISPR proteins, and mutant enrichment, are discussed in detail
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
The review summarizes recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds.
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
The review states that CRISPR-mediated genome engineering has advanced understanding of complex neurologic diseases by enabling rapid generation of disease-relevant in vitro and transgenic animal models.
This technology has advanced our understanding of complex neurologic diseases by enabling the rapid generation of novel, disease-relevant in vitro and transgenic animal models.
The review describes CRISPR-Cas as a powerful genetic tool for genome manipulation across essentially any organism and cell type.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein (CRISPR-Cas) system has emerged as a powerful genetic tool capable of manipulating the genome of essentially any organism and cell type.
The review states that building a reliable CRISPR/Cas genome-engineering system involves the Cas protein, guide RNA, and donor DNA.
key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA
The review discusses limitations of the CRISPR editing system and suggests that future modifications to existing platforms may further advance understanding of the brain.
Additionally, we discuss limitations of the CRISPR editing system and suggest how future modifications to existing platforms may advance our understanding of the brain.
The review describes CRISPR/Cas systems as versatile genomic engineering tools for microbial biotechnology.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools ... for applications in microbial biotechnology.
The review covers many adaptations of the CRISPR platform with emphasis on applications for genetic interrogation of the normal and diseased nervous system.
We begin with an overview of the canonical function of the CRISPR platform, followed by a functional review of its many adaptations, with an emphasis on its applications for genetic interrogation of the normal and diseased nervous system.
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
TAL/TALE proteins and CRISPR/Cas systems have been used extensively for genome editing across cells of various types and species.
Engineered DNA-binding molecules such as transcription activator-like effector (TAL or TALE) proteins and the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) (CRISPR/Cas) system have been used extensively for genome editing in cells of various types and species.
TAL/TALE proteins and CRISPR/Cas systems have been used extensively for genome editing across cells of various types and species.
Engineered DNA-binding molecules such as transcription activator-like effector (TAL or TALE) proteins and the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) (CRISPR/Cas) system have been used extensively for genome editing in cells of various types and species.
TAL/TALE proteins and CRISPR/Cas systems have been used extensively for genome editing across cells of various types and species.
Engineered DNA-binding molecules such as transcription activator-like effector (TAL or TALE) proteins and the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) (CRISPR/Cas) system have been used extensively for genome editing in cells of various types and species.
TAL/TALE proteins and CRISPR/Cas systems have been used extensively for genome editing across cells of various types and species.
Engineered DNA-binding molecules such as transcription activator-like effector (TAL or TALE) proteins and the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) (CRISPR/Cas) system have been used extensively for genome editing in cells of various types and species.
TAL/TALE proteins and CRISPR/Cas systems have been used extensively for genome editing across cells of various types and species.
Engineered DNA-binding molecules such as transcription activator-like effector (TAL or TALE) proteins and the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) (CRISPR/Cas) system have been used extensively for genome editing in cells of various types and species.
TAL/TALE proteins and CRISPR/Cas systems have been used extensively for genome editing across cells of various types and species.
Engineered DNA-binding molecules such as transcription activator-like effector (TAL or TALE) proteins and the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) (CRISPR/Cas) system have been used extensively for genome editing in cells of various types and species.
TAL/TALE proteins and CRISPR/Cas systems have been used extensively for genome editing across cells of various types and species.
Engineered DNA-binding molecules such as transcription activator-like effector (TAL or TALE) proteins and the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) (CRISPR/Cas) system have been used extensively for genome editing in cells of various types and species.
The sequence-specific DNA-binding activities of these engineered DNA-binding molecules can be used for transcriptional activation, transcriptional repression, chromatin modification, visualization of genomic regions, and locus-specific chromatin isolation.
The sequence-specific DNA-binding activities of these engineered DNA-binding molecules can also be utilized for other purposes, such as transcriptional activation, transcriptional repression, chromatin modification, visualization of genomic regions, and isolation of chromatin in a locus-specific manner.
The sequence-specific DNA-binding activities of these engineered DNA-binding molecules can be used for transcriptional activation, transcriptional repression, chromatin modification, visualization of genomic regions, and locus-specific chromatin isolation.
The sequence-specific DNA-binding activities of these engineered DNA-binding molecules can also be utilized for other purposes, such as transcriptional activation, transcriptional repression, chromatin modification, visualization of genomic regions, and isolation of chromatin in a locus-specific manner.
The sequence-specific DNA-binding activities of these engineered DNA-binding molecules can be used for transcriptional activation, transcriptional repression, chromatin modification, visualization of genomic regions, and locus-specific chromatin isolation.
The sequence-specific DNA-binding activities of these engineered DNA-binding molecules can also be utilized for other purposes, such as transcriptional activation, transcriptional repression, chromatin modification, visualization of genomic regions, and isolation of chromatin in a locus-specific manner.
The sequence-specific DNA-binding activities of these engineered DNA-binding molecules can be used for transcriptional activation, transcriptional repression, chromatin modification, visualization of genomic regions, and locus-specific chromatin isolation.
The sequence-specific DNA-binding activities of these engineered DNA-binding molecules can also be utilized for other purposes, such as transcriptional activation, transcriptional repression, chromatin modification, visualization of genomic regions, and isolation of chromatin in a locus-specific manner.
The sequence-specific DNA-binding activities of these engineered DNA-binding molecules can be used for transcriptional activation, transcriptional repression, chromatin modification, visualization of genomic regions, and locus-specific chromatin isolation.
The sequence-specific DNA-binding activities of these engineered DNA-binding molecules can also be utilized for other purposes, such as transcriptional activation, transcriptional repression, chromatin modification, visualization of genomic regions, and isolation of chromatin in a locus-specific manner.
The sequence-specific DNA-binding activities of these engineered DNA-binding molecules can be used for transcriptional activation, transcriptional repression, chromatin modification, visualization of genomic regions, and locus-specific chromatin isolation.
The sequence-specific DNA-binding activities of these engineered DNA-binding molecules can also be utilized for other purposes, such as transcriptional activation, transcriptional repression, chromatin modification, visualization of genomic regions, and isolation of chromatin in a locus-specific manner.
The sequence-specific DNA-binding activities of these engineered DNA-binding molecules can be used for transcriptional activation, transcriptional repression, chromatin modification, visualization of genomic regions, and locus-specific chromatin isolation.
The sequence-specific DNA-binding activities of these engineered DNA-binding molecules can also be utilized for other purposes, such as transcriptional activation, transcriptional repression, chromatin modification, visualization of genomic regions, and isolation of chromatin in a locus-specific manner.
This review focuses on biological applications of engineered DNA-binding molecules other than genome editing.
In this review, we describe applications of these engineered DNA-binding molecules for biological purposes other than genome editing.
This review focuses on biological applications of engineered DNA-binding molecules other than genome editing.
In this review, we describe applications of these engineered DNA-binding molecules for biological purposes other than genome editing.
This review focuses on biological applications of engineered DNA-binding molecules other than genome editing.
In this review, we describe applications of these engineered DNA-binding molecules for biological purposes other than genome editing.
This review focuses on biological applications of engineered DNA-binding molecules other than genome editing.
In this review, we describe applications of these engineered DNA-binding molecules for biological purposes other than genome editing.
This review focuses on biological applications of engineered DNA-binding molecules other than genome editing.
In this review, we describe applications of these engineered DNA-binding molecules for biological purposes other than genome editing.
This review focuses on biological applications of engineered DNA-binding molecules other than genome editing.
In this review, we describe applications of these engineered DNA-binding molecules for biological purposes other than genome editing.
This review focuses on biological applications of engineered DNA-binding molecules other than genome editing.
In this review, we describe applications of these engineered DNA-binding molecules for biological purposes other than genome editing.
Approval Evidence
7 sources23 linked approval claimsfirst-pass slug crispr-cas-system
Recent advancements in genomic studies on Brassica crops and their pathogens have facilitated the deployment of CRISPR/Cas systems in breeding major Brassica crops. This review highlights recent progress in CRISPR/Cas-based gene editing technologies to improve resistance to pathogens and enhance tolerance to drought, salinity, and extreme temperatures.
Source:
Among the diverse gene-editing platforms, the CRISPR-Cas system has emerged as the most extensively employed, owing to its simplicity, low cost, and efficiency.
Source:
With the rapid advancement of genetic modification technologies, the CRISPR/Cas system has demonstrated significant potential in treating viral infections. Its ability to selectively target and edit viral genomes for elimination positions it as a highly effective approach for combating HPV.
Source:
Strategies for High-Efficiency Mutation Using the CRISPR/Cas System
Source:
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools
Source:
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein (CRISPR-Cas) system has emerged as a powerful genetic tool capable of manipulating the genome of essentially any organism and cell type.
Source:
...and the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) (CRISPR/Cas) system...
Source:
application scopesupports
CRISPR-Cas has expanding applications in genetics, biotechnology, agriculture, and medicine.
with a particular emphasis on the CRISPR-Cas system and its expanding applications in genetics, biotechnology, agriculture, and medicine
Source:
application scopesupports
CRISPR/Cas systems have been deployed in breeding major Brassica crops.
Recent advancements in genomic studies on Brassica crops and their pathogens have facilitated the deployment of CRISPR/Cas systems in breeding major Brassica crops.
Source:
capabilitysupports
CRISPR/Cas-based gene editing technologies are being used to improve pathogen resistance and tolerance to drought, salinity, and extreme temperatures in Brassica crops.
This review highlights recent progress in CRISPR/Cas-based gene editing technologies to improve resistance to pathogens and enhance tolerance to drought, salinity, and extreme temperatures.
Source:
comparative adoptionsupports
The CRISPR-Cas system has emerged as the most extensively employed gene-editing platform because of its simplicity, low cost, and efficiency.
Among the diverse gene-editing platforms, the CRISPR-Cas system has emerged as the most extensively employed, owing to its simplicity, low cost, and efficiency.
Source:
research scopesupports
The review discusses a workflow for employing the CRISPR/Cas system to boost stress tolerance and resistance in Brassica species.
Furthermore, the review discusses the workflow for employing the CRISPR/Cas system to boost stress tolerance and resistance, outlines the associated challenges, and explores prospects based on gene editing research in Brassica species.
Source:
therapeutic potentialsupports
The CRISPR/Cas system has significant potential for treating viral infections and is positioned as an effective approach for combating HPV by selectively targeting and editing viral genomes.
Source:
limitation summarysupports
The review states that CRISPR/Cas systems have inherent limitations including off-target effects, unsatisfactory delivery efficiency, and unwanted adverse effects.
CRISPR/Cas systems have some inherent limitations, such as off-target effects, unsatisfactory efficiency of delivery, and unwanted adverse effects
Source:
review scope summarysupports
The review states that CRISPR/Cas systems have revolutionized traditional gene-editing tools and show broad potential for genetic manipulation across many organisms and cell types.
CRISPR/Cas systems have revolutionized traditional gene-editing tools ... have displayed tremendous potential for genetic manipulation in almost any organism and cell type.
Source:
strategy summarysupports
The review describes choice of delivery system as a strategy for improving the efficiency of CRISPR/Cas-induced mutations.
Strategies for improving the efficiency of CRISPR/Cas-induced mutations, such as ... choice of delivery system ... are comprehensively described in this review.
Source:
strategy summarysupports
The review describes improving sgRNA design and modification as a strategy for improving the efficiency of CRISPR/Cas-induced mutations.
Strategies for improving the efficiency of CRISPR/Cas-induced mutations, such as ... improving the design and modification of sgRNA ... are comprehensively described in this review.
Source:
strategy summarysupports
The review discusses Cas variants, anti-CRISPR proteins, and mutant enrichment as newly emerging approaches relevant to improving CRISPR/Cas system use.
several newly emerging approaches, including the use of Cas variants, anti-CRISPR proteins, and mutant enrichment, are discussed in detail
Source:
application scope summarysupports
The review highlights application of the CRISPR/Cas toolbox to multiplexed engineering and high throughput screening.
we highlighted the application of CRISPR/Cas toolbox for multiplexed engineering and high throughput screening
Source:
application scope summarysupports
The review summarizes recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds.
We then summarize recent applications of CRISPR/Cas systems in metabolic engineering toward production of chemicals and natural compounds
Source:
application summarysupports
The review states that CRISPR-mediated genome engineering has advanced understanding of complex neurologic diseases by enabling rapid generation of disease-relevant in vitro and transgenic animal models.
This technology has advanced our understanding of complex neurologic diseases by enabling the rapid generation of novel, disease-relevant in vitro and transgenic animal models.
Source:
capability summarysupports
The review describes CRISPR-Cas as a powerful genetic tool for genome manipulation across essentially any organism and cell type.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein (CRISPR-Cas) system has emerged as a powerful genetic tool capable of manipulating the genome of essentially any organism and cell type.
Source:
design components summarysupports
The review states that building a reliable CRISPR/Cas genome-engineering system involves the Cas protein, guide RNA, and donor DNA.
key points of building reliable CRISPR/Cas system for genome engineering are discussed, including the Cas protein, the guide RNA and the donor DNA
Source:
limitation summarymixed
The review discusses limitations of the CRISPR editing system and suggests that future modifications to existing platforms may further advance understanding of the brain.
Additionally, we discuss limitations of the CRISPR editing system and suggest how future modifications to existing platforms may advance our understanding of the brain.
Source:
review scope summarysupports
The review describes CRISPR/Cas systems as versatile genomic engineering tools for microbial biotechnology.
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools ... for applications in microbial biotechnology.
Source:
scope summarysupports
The review covers many adaptations of the CRISPR platform with emphasis on applications for genetic interrogation of the normal and diseased nervous system.
We begin with an overview of the canonical function of the CRISPR platform, followed by a functional review of its many adaptations, with an emphasis on its applications for genetic interrogation of the normal and diseased nervous system.
Source:
toolkit scope summarysupports
The review covers CRISPR/Cas tools for gene activation, gene interference, orthogonal CRISPR systems, and precise single base editing.
various CRISPR/Cas tools for genome engineering, including gene activation, gene interference, orthogonal CRISPR systems and precise single base editing
Source:
Comparisons
Source-backed strengths
The evidence describes CRISPR/Cas as a versatile genomic engineering tool and a powerful genetic platform capable of manipulating the genome of essentially any organism and cell type. Its utility is further supported by multiple improvement avenues discussed in the review, including sgRNA optimization, Cas variants, anti-CRISPR proteins, and edited-mutant enrichment.
Ranked Citations
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