Source 1primary paper2017Frontiers in Molecular Neuroscience
Toolkit/CRISPR/Cas9 genome editing technique
CRISPR/Cas9 genome editing technique
Also known as: clustered regularly interspaced short palindromic repeat (CRISPR)-associated Cas9 nuclease (CRISPR/Cas9)
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
CRISPR/Cas9 is a genome editing technique used in the cited study to generate Cib1 and Cib2 knockout mice. In this evidence set, its demonstrated function is targeted gene disruption for mouse model production.
Usefulness & Problems
Why this is useful
This technique is useful for producing defined gene knockout mouse lines for functional studies in vivo. Here, it enabled generation of Cib1 and Cib2 loss-of-function models that were used to assess auditory phenotypes and hair-cell mechanoelectrical transduction requirements.
Problem solved
It addresses the need to disrupt specific genes in mice to test gene function in a whole-animal context. In the cited work, it solved the problem of establishing Cib1 and Cib2 knockout models for phenotypic analysis.
Problem links
Need controllable genome or transcript editing
DerivedCRISPR/Cas9 is a genome editing technique that was used in the cited study to generate Cib1 and Cib2 knockout mice. In this evidence set, its demonstrated function is targeted gene disruption for mouse model production.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Techniques
No technique tags yet.
Target processes
editingInput: Electrical
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenoperating role: builderswitch architecture: multi component
The source text specifies use of the clustered regularly interspaced short palindromic repeat-associated Cas9 nuclease genome editing technique to establish knockout mice. This evidence does not provide construct design, guide RNA sequences, delivery method, strain background, or embryo manipulation details.
The evidence set only documents use of CRISPR/Cas9 for knockout mouse generation and does not report editing efficiency, on-target lesion structure, off-target analysis, or comparison with alternative editing methods. No practical performance metrics or replication across multiple studies are provided here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Observations
failedMousefailed attemptmouse
Inferred from claim c3 during normalization. Loss of CIB2 causes profound hearing loss in mice. Derived from claim c3. Quoted text: loss of CIB2 protein causes profound hearing loss in mice
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Supporting Sources
Ranked Claims
Hair cell stereocilia development is affected in Cib2 knockout mice.
hair cell stereocilia development is affected in Cib2 knockout mice
CIB2 is essential for auditory hair cell mechanoelectrical transduction.
CIB2 is essential for auditory hair cell MET
Loss of CIB1 does not affect auditory function in mice.
We found that loss of CIB1 protein does not affect auditory function
Loss of CIB2 causes profound hearing loss in mice.
loss of CIB2 protein causes profound hearing loss in mice
Cib1 and Cib2 knockout mice were established using CRISPR/Cas9 genome editing.
we established Cib1 and Cib2 knockout mice using the clustered regularly interspaced short palindromic repeat (CRISPR)-associated Cas9 nuclease (CRISPR/Cas9) genome editing technique
Loss of CIB2 abolishes mechanoelectrical transduction currents in auditory hair cells.
loss of CIB2 abolishes mechanoelectrical transduction (MET) currents in auditory hair cells
Approval Evidence
1 source6 linked approval claimsfirst-pass slug crispr-cas9-genome-editing-technique
we established Cib1 and Cib2 knockout mice using the clustered regularly interspaced short palindromic repeat (CRISPR)-associated Cas9 nuclease (CRISPR/Cas9) genome editing technique
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developmental phenotypesupports
Hair cell stereocilia development is affected in Cib2 knockout mice.
hair cell stereocilia development is affected in Cib2 knockout mice
Source:
gene essentialitysupports
CIB2 is essential for auditory hair cell mechanoelectrical transduction.
CIB2 is essential for auditory hair cell MET
Source:
gene function phenotypesupports
Loss of CIB1 does not affect auditory function in mice.
We found that loss of CIB1 protein does not affect auditory function
Source:
gene function phenotypesupports
Loss of CIB2 causes profound hearing loss in mice.
loss of CIB2 protein causes profound hearing loss in mice
Source:
gene knockout generation methodsupports
Cib1 and Cib2 knockout mice were established using CRISPR/Cas9 genome editing.
we established Cib1 and Cib2 knockout mice using the clustered regularly interspaced short palindromic repeat (CRISPR)-associated Cas9 nuclease (CRISPR/Cas9) genome editing technique
Source:
mechanistic requirementsupports
Loss of CIB2 abolishes mechanoelectrical transduction currents in auditory hair cells.
loss of CIB2 abolishes mechanoelectrical transduction (MET) currents in auditory hair cells
Source:
Comparisons
Source-backed strengths
The evidence shows successful establishment of both Cib1 and Cib2 knockout mice using CRISPR/Cas9. These models supported biologically informative downstream experiments, including demonstration that CIB2 is required for auditory hair-cell mechanoelectrical transduction and that Cib2 loss causes profound hearing loss, whereas Cib1 loss does not affect auditory function.
Compared with CRISPR/Cas
CRISPR/Cas9 genome editing technique and CRISPR/Cas address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; shared mechanisms: genome editing
Compared with CRISPR/Cas9 mediated genome editing
CRISPR/Cas9 genome editing technique and CRISPR/Cas9 mediated genome editing address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; shared mechanisms: genome editing
Compared with duplex CRISPR/Cas9 technology
CRISPR/Cas9 genome editing technique and duplex CRISPR/Cas9 technology address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; shared mechanisms: genome editing
Ranked Citations
- 1.