Source 1primary paper2016Scientific Reports
Toolkit/CRISPR/Cas9 mediated genome editing
CRISPR/Cas9 mediated genome editing
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
CRISPR/Cas9 mediated genome editing is a genome engineering method used in the cited 2016 Scientific Reports study to investigate the functional role of human Interleukin-8 gene haplotypes. The supplied evidence supports its application in editing a specific human gene-haplotype context.
Usefulness & Problems
Why this is useful
The available evidence indicates that this method is useful for experimentally interrogating the functional consequences of human IL8 haplotypes through targeted genome modification. Beyond that study objective, the provided evidence does not describe additional use cases or performance characteristics.
Problem solved
This method addresses the problem of testing the functional role of human Interleukin-8 gene haplotypes by enabling genome editing in that locus. The supplied evidence does not specify the exact edit design, cellular system, or downstream assay used to resolve haplotype function.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete method used to build, optimize, or evolve an engineered system.
Mechanisms
genome editingTechniques
No technique tags yet.
Target processes
editingImplementation Constraints
The supplied evidence identifies CRISPR/Cas9 mediated genome editing as the method but does not report construct architecture, guide RNA design, delivery modality, donor template use, or host cell type. Practical implementation details are therefore not available from the provided material.
The evidence is limited to the study title and objective, so there is no direct information on editing efficiency, off-target activity, repair outcomes, or reproducibility. Validation is therefore confined to a single reported application involving human Interleukin-8 haplotypes.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The study investigates the functional role of human Interleukin-8 gene haplotypes using CRISPR/Cas9 mediated genome editing.
The study investigates the functional role of human Interleukin-8 gene haplotypes using CRISPR/Cas9 mediated genome editing.
The study investigates the functional role of human Interleukin-8 gene haplotypes using CRISPR/Cas9 mediated genome editing.
The study investigates the functional role of human Interleukin-8 gene haplotypes using CRISPR/Cas9 mediated genome editing.
The study investigates the functional role of human Interleukin-8 gene haplotypes using CRISPR/Cas9 mediated genome editing.
The study investigates the functional role of human Interleukin-8 gene haplotypes using CRISPR/Cas9 mediated genome editing.
The study investigates the functional role of human Interleukin-8 gene haplotypes using CRISPR/Cas9 mediated genome editing.
Approval Evidence
1 source1 linked approval claimfirst-pass slug crispr-cas9-mediated-genome-editing
Investigation of the functional role of human Interleukin-8 gene haplotypes by CRISPR/Cas9 mediated genome editing
Source:
study objectivesupports
The study investigates the functional role of human Interleukin-8 gene haplotypes using CRISPR/Cas9 mediated genome editing.
Source:
Comparisons
Source-backed strengths
A clear strength supported by the evidence is that CRISPR/Cas9 was sufficiently applicable to be used as the central genome engineering approach in a study of human IL8 haplotypes. No quantitative editing outcomes, specificity data, or comparative advantages are provided in the supplied evidence.
Ranked Citations
- 1.